Leicester Tigers second row Graham Kitchener will captain the England selection which includes players from seven Premiership sides.
Henry Slade, the 21 year-old Exeter Chiefs fly half will be vice-captain.
RFU Head of International Player Development Joe Lydon said: "This England squad is full of talent and will make for an entertaining match against a strong Barbarians side with over 700 caps between them.
"We see this match as an important development opportunity for the players and many of them gained valuable experience working with the senior squad and coaches over the last week before they departed for New Zealand. We have seen 10 players involved in these Barbarians fixtures over the last two years who have gone on to get caps for England.
"The team are really looking forward to running out and playing in front of the home crowd at Twickenham which has had a fantastic atmosphere this season."
England XV (v Barbarians, Twickenham Stadium, Sunday 1 June, 3pm)
15 Elliot Daly (London Wasps)
14 Semesa Rokoduguni (Bath Rugby & Army)
13 Jonathan Joseph (Bath Rugby)
12 Sam Hill (Exeter Chiefs)
11 Charlie Sharples (Gloucester Rugby)
10 Henry Slade (Exeter Chiefs)
9 Joe Simpson (London Wasps)
1 Ross Harrison (Sale Sharks)
2 Rob Buchanan (Harlequins)
3 Kyle Sinckler (Harlequins)
4 Michael Paterson (Sale Sharks)
5 Graham Kitchener (Leicester Tigers)
6 Jamie Gibson (Leicester Tigers)
7 Luke Wallace (Harlequins)
8 Dave Ewers (Exeter Chiefs)
16 Tommy Taylor (Sale Sharks)
17 Fraser Balmain (Leicester Tigers)
18 Will Collier (Harlequins)
19 Charlie Matthews (Harlequins)
20 James Gaskell (Sale Sharks)
21 Dan Robson (Gloucester Rugby)
22 Ollie Devoto (Bath Rugby)
23 Rob Miller (Sale Sharks)