Joining the Cherry and Whites in Pool B alongside South Africans Vodacom Blue Bulls will be Cardiff Blues and Buenos Aires as the men from Kingsholm attempt to improve on their 6th placed finish in last year's tournament when New York City 7s defeated Rob Thirlby's men in the Plate Final.
Tickets for the event at Twickenham are available to purchase here
Each of the three pools has a cosmopolitan feel as 12 of the best club 7s sides from across the world battle it out over two days of high-octane rugby.
New York - last year's Plate Winners - have been handed the privilege of kicking off the tournament at 11.30am on Saturday, taking on Siberian side RC Enisei, while the heavyweight clash between Auckland and Super 15 finalists NSW Waratahs closes day one at 6.30pm.
In between thousands of fans will be treated to a feast of 19 different 7s rugby clashes that include an exhibition game between the Army and a Rugby For Heroes select side.
All teams will compete against each other on day one, before dividing into three Cup competitions on day two, with the leading sides competing for the chance to be crowned the best club 7s side in rugby.
Siberia's RC Enisei, Australia's NSW Waratahs and USA's Seattle will join Cardiff Blues for their World Club 7s debuts. Vodacom Blue Bulls, Auckland, DHL Western Province, New York City 7s, Gloucester Rugby, Kuban Krasnodar and Buenos Aires are among the teams making a return to Twickenham for this year's tournament.
The 12th side, who will go into Pool A alongside RC Enisei and DHL Western Province will be confirmed on the evening of Friday 8 August, at the final of the Premiership Rugby 7s competition.
Live BT Sport coverage starts on Saturday at 3pm when Gloucester Rugby take on Cardiff Blues, followed by an intriguing clash between the Vodacom Blue Bulls and Buenos Aires.
The knockout matches start at 11.30am on Sunday with the Final kicking off just after 5.30pm. The exhibition match on the Sunday will see two top-class women's teams meet for The Supporters Club Trophy.