LESLEY VAINIKOLO has been ruled out of tomorrow's Guinness Premiership showdown against European champions Wasps because of a knee injury.
The giant winger, who is the Premiership's leading try-scorer with seven in five appearances, suffered a blow to his left knee against Ulster last week and is not considered for selection.
"Les took a bang to the knee last week and although the injury is not that serious it will prevent him playing against Wasps," said Gloucester's head coach Dean Ryan.
"It's not a long-term issue and he will link up with England as planned. It is obviously a significant blow to us but we are not going to take risks with a player like Les. In a season of this length and intensity you pick up injuries - we just have to manage that."
Ryan has been forced into a series of changes tomorrow and will unveil a new second row combination because of injuries to Alex Brown (arm) and Marco Bortolami (eye socket).
But there is a return for flanker Andy Hazell, who has been out of action since the end of November with knee trouble. He comes in on the openside flank for Akapusi Qera, who was not considered for selection because of a sceptic cut on his hand.
"Andy is a fantastic servant to this club - a Gloucester man who understands the significance of wearing a Gloucester shirt - and he brings a huge level intensity and desire to our efforts," Ryan said.
"We are fortunate to have great options in our pack but Andy's return is very timely and it will be fantastic to see him involved again."
Hazell will pack down alongside Luke Narraway at six and Gareth Delve at eight. Peter Buxton will skipper the side from the second row alongside Will James.
The front row of Nick Wood, Olivier Azam and Carlos Nieto remains unchanged.
There are also changes in the backline. With Vainikolo out, Iain Balshaw switches to the wing and Willie Walker comes in at full-back. Anthony Allen, who has featured in 17 of Gloucester's 18 matches this term, moves to the bench which means James Simpson-Daniel comes into the centre to partner Mike Tindall.
Replacing Simpson-Daniel on the wing is James Bailey, who will make his fifth Premiership appearance of the season.
Rory Lawson and Ryan Lamb are the half-backs.
"There are some changes and we have obviously been disrupted by injuries but I think our set-up is exciting and carries a lot of potential," Ryan added. "We are obviously aware of what Wasps represent and are expecting a reaction from their defeat last weekend.
"This is traditionally the stage of the season where they come into their own - they are a fantastic side full of fantastic players and we know we will have to be very competitive to win."
On the bench, Jeremy Paul and Patrice Collazo are the front row options, lock Dan Tuohy comes into the squad for the first time this season and Alasdair Strokosch completes the forwards.
Gareth Cooper, Allen and Mark Foster are Gloucester's three-quarter options. Chris Paterson is ruled out with a dead leg.
GLOUCESTER (v Wasps, tomorrow, 3pm): Willie Walker, Iain Balshaw, James Simpson-Daniel, Mike Tindall, James Bailey; Ryan Lamb, Rory Lawson; Nick Wood, Olivier Azam, Carlos Nieto, Peter Buxton (capt), Will James, Luke Narraway, Andy Hazell, Gareth Delve. Reps: Jeremy Paul, Patrice Collazo, Dan Tuohy, Alasdair Strokosch, Gareth Cooper, Anthony Allen, Mark Foster.
Despite he and a number of his team mates playing out of position, Greig Laidlaw's four penalties were enough to help Gloucester to a 12-11 Aviva Premiership win at Sale on Friday evening. The skipper told GRTV after the game that everyone had played their part, especially the younger players.
Greig Laidlaw skippers the Cherry and Whites on Friday evening, when they head to Sale Sharks for the penultimate game of this season's Aviva Premiership. The Scottish half back spoke to GRTV about the challenge posed in Salford.
Previewing Friday night's Aviva Premiership fixture away at Sale Sharks, Head Coach Laurie Fisher acknowledged the fine home record that the Salford based outfit have built up this season, but is looking for Gloucester to round off their campaign on a high note.
Speaking to GRTV following the 16-9 victory over Exeter, Tom Savage felt that Gloucester needed that win after a tough few weeks and hopes that it acts as a springboard to a good end to the season for the Cherry and Whites.
A week on from '...letting down ourselves, the jersey and the supporters...', skipper Greig Laidlaw was full of praise for his team mates following the 16-9 Aviva Premiership win over Exeter Chiefs at Kingsholm on Friday evening.
Four penalties from the boot of skipper Greig Laidlaw were just enough to see Gloucester dog out a tense 12-11 win at Sale on Friday evening and thwart the home team's attempts to finish the season unbeaten at home.
Thirteen years on from Gloucester Rugby's famous Powergen Cup victory over Northampton at Twickenham, many of the players and management from that side are gathering once more to recreate that famous day.