LESLEY VAINIKOLO has been ruled out of tomorrow's Guinness Premiership showdown against European champions Wasps because of a knee injury.
The giant winger, who is the Premiership's leading try-scorer with seven in five appearances, suffered a blow to his left knee against Ulster last week and is not considered for selection.
"Les took a bang to the knee last week and although the injury is not that serious it will prevent him playing against Wasps," said Gloucester's head coach Dean Ryan.
"It's not a long-term issue and he will link up with England as planned. It is obviously a significant blow to us but we are not going to take risks with a player like Les. In a season of this length and intensity you pick up injuries - we just have to manage that."
Ryan has been forced into a series of changes tomorrow and will unveil a new second row combination because of injuries to Alex Brown (arm) and Marco Bortolami (eye socket).
But there is a return for flanker Andy Hazell, who has been out of action since the end of November with knee trouble. He comes in on the openside flank for Akapusi Qera, who was not considered for selection because of a sceptic cut on his hand.
"Andy is a fantastic servant to this club - a Gloucester man who understands the significance of wearing a Gloucester shirt - and he brings a huge level intensity and desire to our efforts," Ryan said.
"We are fortunate to have great options in our pack but Andy's return is very timely and it will be fantastic to see him involved again."
Hazell will pack down alongside Luke Narraway at six and Gareth Delve at eight. Peter Buxton will skipper the side from the second row alongside Will James.
The front row of Nick Wood, Olivier Azam and Carlos Nieto remains unchanged.
There are also changes in the backline. With Vainikolo out, Iain Balshaw switches to the wing and Willie Walker comes in at full-back. Anthony Allen, who has featured in 17 of Gloucester's 18 matches this term, moves to the bench which means James Simpson-Daniel comes into the centre to partner Mike Tindall.
Replacing Simpson-Daniel on the wing is James Bailey, who will make his fifth Premiership appearance of the season.
Rory Lawson and Ryan Lamb are the half-backs.
"There are some changes and we have obviously been disrupted by injuries but I think our set-up is exciting and carries a lot of potential," Ryan added. "We are obviously aware of what Wasps represent and are expecting a reaction from their defeat last weekend.
"This is traditionally the stage of the season where they come into their own - they are a fantastic side full of fantastic players and we know we will have to be very competitive to win."
On the bench, Jeremy Paul and Patrice Collazo are the front row options, lock Dan Tuohy comes into the squad for the first time this season and Alasdair Strokosch completes the forwards.
Gareth Cooper, Allen and Mark Foster are Gloucester's three-quarter options. Chris Paterson is ruled out with a dead leg.
GLOUCESTER (v Wasps, tomorrow, 3pm): Willie Walker, Iain Balshaw, James Simpson-Daniel, Mike Tindall, James Bailey; Ryan Lamb, Rory Lawson; Nick Wood, Olivier Azam, Carlos Nieto, Peter Buxton (capt), Will James, Luke Narraway, Andy Hazell, Gareth Delve. Reps: Jeremy Paul, Patrice Collazo, Dan Tuohy, Alasdair Strokosch, Gareth Cooper, Anthony Allen, Mark Foster.
In the second episode in the series of videos where Gloucester Rugby TV chat to box holders at Kingsholm, after they note twenty years as owners of a box. Nick Broady of Avenue Cars and Paul Baldwin of Brunsdons chat to the camera about their memories and about rugby in general.
Gary Jones of Glevum Windows and Conservatories recently visited Kingsholm Stadium and the Cherry and White supporter spoke exclusively to Gloucester Rugby TV after marking two decades as a box holder in the Stowford Press Stand at Kingsholm. A great feat!
Speaking to the local media this week during preseason, new hooker Richard Hibbard said he was really enjoying putting in the hard work and being back to full fitness ahead of the 2014-15 season with the Cherry and Whites.
Nick Walshe was unveiled as Gloucester's new Backs and Attack Coach this week and spoke to Simon Ward for the RFU.com podcast about his excitement at joining Gloucester and working with a new group of players.
Speaking after the Third Test defeat to New Zealand, Ben Morgan told RFU.com that the loss was a disappointing way to sign off the summer tour but that the England team know what they have to do to be successful.
At the end of a tour when there have been so many positives for England, fly half Freddie Burns, who will start the third Test against New Zealand, was clear that the whole squad are focussed on signing off with a memorable win when he spoke to RFU.com
Speaking to Steve Jones of RFU.com straight after the 27-28 defeat to New Zealand on Saturday, Billy Twelvetrees spoke of the frustration of a missed opportunity, but stressed that England would lick their wounds and bounce back.
Freddie Burns contributed 12 points in England's 20-15 defeat against New Zealand on Saturday but, speaking after the game to RFU.com, preferred to talk about the many positives in the performance from Stuart Lancaster's side.
Gloucester number eight Ben Morgan put in a great individual performance as England were just pipped by New Zealand by 20 points to 15 in Auckland on Saturday. He told Steve Jones of RFU.com that the team will be in good spirits going into the 2nd Test in Dunedin.
Gloucester Rugby are fully aware of a delay in the delivery of a small number of club membership packs to supporters ahead of the Premiership Rugby 7s on Thursday evening and would like to issue the following advice.
Thursday will present the first chance for Gloucester Rugby supporters to watch new signing Jacob Rowan in a Cherry and White shirt as the back rower runs out for the first time in the Premiership Rugby Sevens.
Tickets are selling fast for the Premiership Rugby 7s at Kingsholm on Thursday 31 July, so to guarantee your ticket for the return of the action at the 2014-15 season's curtain raiser, the ticket office are opening late on Wednesday evening.
Thursday evening sees the return of live action to Kingsholm in the form of the Premiership Rugby 7s and the Cherry and Whites have named an exciting squad ready to take on Bath Rugby, Exeter Chiefs and London Irish in Pool B.