GLOUCESTER will field the same starting XV that beat Bath last Saturday when they face-off against Leicester in the Guinness Premiership semi-final on Sunday at Kingsholm (4.30pm).
Concerns over their injured trio of Mike Tindall, Anthony Allen and Akapusi Qera have eased and all three will feature against the reigning champions.
Tindall limped out of Saturday's 8-6 west country derby win over Bath with an ankle injury, while Allen and Qera have both recovered from shoulder complaints in time to take their place in the side.
"In a defensive performance of last weekend's quality and intensity there were always going to be question marks over certain individuals but we are obviously very pleased everyone has come through," head coach Dean Ryan told www.gloucesterrugby.co.uk
"We tried to get most of our preparation and class room work done to give people enough time to recover in the early part of the week because we had some bumps and bruises.
"The play-offs are a brutal environment but we are learning to operate at a higher level and that is what we must remember. Leicester are an outstanding side because they have been in this situation many times before and it will be nothing new to them.
"But we are in a confident frame of mind given the form and results we have produced in the last few weeks and we must take that forward into Sunday.
"It is up to us to go and produce another performance and if we can do that then I am sure we can create a threat."
Gloucester's forwards are made up of loose-head prop Nick Wood, hooker Andy Titterrell and tight-head prop Carlos Nieto.
Marco Bortolami and Alex Brown will partner each other at lock for the sixth time in seven matches, while Alasdair Strokosch will wear number six, Qera - who has scored four tries in the last five matches - will wear seven and Gareth Delve eight.
In the backs, scrum-half Rory Lawson will partner Ryan Lamb at half-back. Anthony Allen and Mike Tindall will be the centre combination and James Simpson-Daniel, Lesley Vainikolo and Iain Balshaw the back three.
Gloucester will make one change from their 22-man squad that beat Bath and that is on the bench. Jack Forster comes into the squad in place of Scotland's Alasdair Dickinson.
Forster's last start was away to Sale Sharks in March and he will be joined by hooker Olivier Azam, lock Will James and number eight Luke Narraway.
In the backs, Ryan has gone for scrum-half Gareth Cooper, utility back Willie Walker and winger Mark Foster.
GLOUCESTER (v Leicester, Kingsholm, Sunday, 4.30pm): Iain Balshaw; James Simpson-Daniel, Mike Tindall, Anthony Allen, Lesley Vainikolo; Ryan Lamb, Rory Lawson; Nick Wood, Andy Titterrell, Carlos Nieto, Marco Bortolami (capt), Alex Brown, Alasdair Strokosch, Akapusi Qera, Gareth Delve. Reps: Olivier Azam, Jack Forster, Will James, Luke Narraway, Gareth Cooper, Willie Walker, Mark Foster.
Gloucester look to build upon the local derby win over Bath last weekend with Harlequins the visitors to Kingsholm on Saturday (kick off 3pm). Director of Rugby David Humphreys is expecting a close game with the two teams well matched, although it may not be a 78 point thriller like the last time the two teams met.
Named Man of the Match by BT Sport, scrum-half Willi Heinz said after the 15-11 win at Bath on Friday evening that it was a pretty happy Gloucester dressing room and reward for a lot of hard work put in during the week leading up to the game.
Speaking after the 15-11 win over Bath at the Rec on Friday evening, David Humphreys heaped praise on his players, pointing out how they had bounced back after last week's tough defeat to overcome a rough start before controlling the game and earning the win.
Gloucester Rugby hooker Richard Hibbard spoke to the local media this week as the Cherry and Whites prepare to face Bath Rugby in the Aviva Premiership. The Welshman is ready to bounce back at The Rec.
Speaking to the local media about the local derby against Bath at the Rec on Friday evening, Director of Rugby David Humphreys acknowledged that it will be a tough encounter but hugely exciting at the same time and one the squad are looking forward to.
A visibly disappointed Billy Twelvetrees admitted that it was a very sombre Gloucester dressing room as a last minute try and conversion condemned the Cherry and Whites to an 18-19 defeat against Leicester Tigers on Saturday.
A disappointed Tom Savage told GRTV, after the 18-19 defeat at home to the Leicester Tigers, that the whole Gloucester team had put in a fantastic effort, but just couldn't find the extra one or two percent necessary to put away a top side.
Speaking after the narrow loss to Leicester Tigers on Saturday afternoon, David Humphreys says despite there being a lot of good in Gloucester's performance, he rued the failure to close the game out, as well as thanking the home support
After a disappointing opening weekend loss to their Scottish counterparts, England U20s will be eager to bounce back in Italy this evening, with Gloucester flyer Mat Protheroe set to pull the strings from fly half.
Gloucester Rugby make just the one change to the starting line up that won at Bath last Friday evening, flanker Matt Kvesic restored to the starting XV as the Cherry and Whites welcome Harlequins to Kingsholm in the Aviva Premiership on Saturday (kick off 3pm).
The Early Bird deadline for ticket purchases for the Aviva Premiership fixture against Harlequins on Saturday will expire at 6:30pm on Thursday evening, with tickets already limited for one area of Kingsholm.