GLOUCESTER will field the same starting XV that beat Bath last Saturday when they face-off against Leicester in the Guinness Premiership semi-final on Sunday at Kingsholm (4.30pm).
Concerns over their injured trio of Mike Tindall, Anthony Allen and Akapusi Qera have eased and all three will feature against the reigning champions.
Tindall limped out of Saturday's 8-6 west country derby win over Bath with an ankle injury, while Allen and Qera have both recovered from shoulder complaints in time to take their place in the side.
"In a defensive performance of last weekend's quality and intensity there were always going to be question marks over certain individuals but we are obviously very pleased everyone has come through," head coach Dean Ryan told www.gloucesterrugby.co.uk
"We tried to get most of our preparation and class room work done to give people enough time to recover in the early part of the week because we had some bumps and bruises.
"The play-offs are a brutal environment but we are learning to operate at a higher level and that is what we must remember. Leicester are an outstanding side because they have been in this situation many times before and it will be nothing new to them.
"But we are in a confident frame of mind given the form and results we have produced in the last few weeks and we must take that forward into Sunday.
"It is up to us to go and produce another performance and if we can do that then I am sure we can create a threat."
Gloucester's forwards are made up of loose-head prop Nick Wood, hooker Andy Titterrell and tight-head prop Carlos Nieto.
Marco Bortolami and Alex Brown will partner each other at lock for the sixth time in seven matches, while Alasdair Strokosch will wear number six, Qera - who has scored four tries in the last five matches - will wear seven and Gareth Delve eight.
In the backs, scrum-half Rory Lawson will partner Ryan Lamb at half-back. Anthony Allen and Mike Tindall will be the centre combination and James Simpson-Daniel, Lesley Vainikolo and Iain Balshaw the back three.
Gloucester will make one change from their 22-man squad that beat Bath and that is on the bench. Jack Forster comes into the squad in place of Scotland's Alasdair Dickinson.
Forster's last start was away to Sale Sharks in March and he will be joined by hooker Olivier Azam, lock Will James and number eight Luke Narraway.
In the backs, Ryan has gone for scrum-half Gareth Cooper, utility back Willie Walker and winger Mark Foster.
GLOUCESTER (v Leicester, Kingsholm, Sunday, 4.30pm): Iain Balshaw; James Simpson-Daniel, Mike Tindall, Anthony Allen, Lesley Vainikolo; Ryan Lamb, Rory Lawson; Nick Wood, Andy Titterrell, Carlos Nieto, Marco Bortolami (capt), Alex Brown, Alasdair Strokosch, Akapusi Qera, Gareth Delve. Reps: Olivier Azam, Jack Forster, Will James, Luke Narraway, Gareth Cooper, Willie Walker, Mark Foster.
After his last ditch try clinched the 42-40 Aviva Premiership victory over Newcastle at Kingsholm on Saturday, Billy Burns told GRTV that all the Gloucester team deserve credit for hanging in there against a Falcons team who really made life tough for the Cherry and Whites.
Speaking ahead of Saturday's Aviva Premiership fixture at home to Newcastle Falcons (kick off 3pm), Director of Rugby David Humphreys emphasised the importance this weekend of Gloucester building on last week's impressive home victory against the Exeter Chiefs.
Speaking to the assembled national and local media, try-scorer Jonny May was delighted and relieved to have tasted victory on Saturday evening, and is looking forward to the European Rugby Challenge Cup final.
Tom Savage delivered a Man of the Match performance against Exeter Chiefs on his birthday on Saturday evening. He spoke to GRTV after the game about the importance of the European Rugby Challenge Cup win.
Speaking to the local media this week, John Afoa described the hard work that the Cherry and White have been putting in and that he is fighting fit ready for Saturday's European Rugby Challenge Cup semi final clash with Exeter Chiefs.
Gloucester Rugby face Exeter Chiefs this Saturday in the European Rugby Challenge Cup semi-final, and Charlie Sharples, who starts at full back is relishing the challenge of the big Kingsholm occasion.
Gloucester Rugby return to Aviva Premiership action against the Newcastle Falcons on Saturday (Kingsholm, kick off 3pm), and make several changes to the side that defeated Exeter Chiefs at home last weekend.