England have named their "strongest available" 26-man squad for the first game in the RBS Under 20 Six Nations.
Head coach Mark Mapletoft's side face Wales in the opening round at Gloucester this Friday night (7.45pm).
Lock James Gaskell (Sale Sharks), full back Tom Homer (London Irish) and fly half Rory Clegg (Harlequins) return from first team duty in the Heineken Cup and Amlin Cup.
All three started last summer's IRB Junior World Championship final against New Zealand in Tokyo but missed last week's 61-7 warm-up win against Cambridge University, when England ran in nine tries against the students.
Several players were involved in LV= Cup games on Saturday and Sunday, shortening England's preparation time for the game at Kingsholm.
"We feel we've got our strongest available squad, which is always positive, because we know what a tough contest Wales will be for us."
"Fourteen of the 26 have been capped at this level before and that experience will be invaluable."
"Personally, as a player and a coach, games against Wales are always the ones I look forward to most eagerly. The rivalry always gets the pulses racing, particularly down at Kingsholm, and I think it'll be a special occasion."
"The five day turnaround will be good experience for the players and if we get our heads down and work hard everything else will look after itself."
Six players from the 32-man Under 20 Elite Player Squad miss out.
Of the backs, Christian Wade (London Wasps) is with the England Sevens squad, Jordi Pasqualin (Gloucester Rugby) and Marcus Watson (London Irish) are injured, and forwards Mako Vunipola (Bristol Rugby), Kieran Brookes (Newcastle Falcons) and Peter Elder (London Wasps) stand down.
Joe Marler (Harlequins), Lee Imiolek (Sale Sharks), Jamie George (Saracens), Arthur Ellis (London Wasps), Shaun Knight (Gloucester Rugby), Calum Green (Leicester Tigers), Danny Wright (Gloucester Rugby), James Gaskell (Sale Sharks), Charlie Matthews (Harlequins), Jamie Gibson (London Irish), Jacob Rowan (Leeds Carnegie), Will Welch (Newcastle Falcons), Alex Gray (Newcastle Falcons), Jackson Wray (Saracens)
Sam Harrison (Leicester Tigers), Sam Stuart (Harlequins), Freddie Burns (Gloucester Rugby), Rory Clegg (Harlequins), Jake Sharp (Saracens), Tom Casson (Saracens), Tom Catterick (Newcastle Falcons), Jonny May (Gloucester Rugby), Jonathan Joseph (London Irish), Sam Smith (Harlequins), Will Hurrell (Leicester Tigers), Tom Homer (London Irish)
England's RBS U20 Six Nations fixtures (local times)
Feb 5: England U20 v Wales U20 (Gloucester, 7.45pm) live on Sky Sports
Feb 12: Italy U20 v England U20 (San Dona di Piave, 7pm)
Feb 26: England U20 v Ireland U20 (Gloucester, 7.45pm)
Mar 12: Scotland U20 v England U20 (Firhill, 7.30pm)
Mar 21: France U20 v England U20 (St Nazaire, 3pm)
Tickets are still available and priced as follows:
Seats in the C&G and JS Stands: Adult £15, Concession £12 and Juniors £5.
Standing in the Shed and West Terrace: Adult £10, Concession £8 and Juniors £4.
Gloucester Rugby season ticket holders will be able to purchase tickets at the following discounted prices:
Adult £13, Concession £10 and Juniors £3 in the C&G and JS Stands.
Adult £8, Concession £6 and Juniors £2 in the Shed and West Terrace.
Supporters can also save money by booking the double match package priced as follows:
Adult £26, Concessions £20 and Juniors £6 in the C&G and JS Stands.
Adults £16, Concession £12 and Juniors £4 in the Shed and West Terrace.
A number of hospitality packages are still available for both matches in the C&G Stand and West Stand. Prices start from £500 plus VAT for a 12 person suite.
For more information call the Commerical sales team on 0871 871 8781 option 4 or email firstname.lastname@example.org
Gloucester look to build upon the local derby win over Bath last weekend with Harlequins the visitors to Kingsholm on Saturday (kick off 3pm). Director of Rugby David Humphreys is expecting a close game with the two teams well matched, although it may not be a 78 point thriller like the last time the two teams met.
Named Man of the Match by BT Sport, scrum-half Willi Heinz said after the 15-11 win at Bath on Friday evening that it was a pretty happy Gloucester dressing room and reward for a lot of hard work put in during the week leading up to the game.
Speaking after the 15-11 win over Bath at the Rec on Friday evening, David Humphreys heaped praise on his players, pointing out how they had bounced back after last week's tough defeat to overcome a rough start before controlling the game and earning the win.
Gloucester Rugby hooker Richard Hibbard spoke to the local media this week as the Cherry and Whites prepare to face Bath Rugby in the Aviva Premiership. The Welshman is ready to bounce back at The Rec.
Speaking to the local media about the local derby against Bath at the Rec on Friday evening, Director of Rugby David Humphreys acknowledged that it will be a tough encounter but hugely exciting at the same time and one the squad are looking forward to.
A visibly disappointed Billy Twelvetrees admitted that it was a very sombre Gloucester dressing room as a last minute try and conversion condemned the Cherry and Whites to an 18-19 defeat against Leicester Tigers on Saturday.
A disappointed Tom Savage told GRTV, after the 18-19 defeat at home to the Leicester Tigers, that the whole Gloucester team had put in a fantastic effort, but just couldn't find the extra one or two percent necessary to put away a top side.
Speaking after the narrow loss to Leicester Tigers on Saturday afternoon, David Humphreys says despite there being a lot of good in Gloucester's performance, he rued the failure to close the game out, as well as thanking the home support
After a disappointing opening weekend loss to their Scottish counterparts, England U20s will be eager to bounce back in Italy this evening, with Gloucester flyer Mat Protheroe set to pull the strings from fly half.
Gloucester Rugby make just the one change to the starting line up that won at Bath last Friday evening, flanker Matt Kvesic restored to the starting XV as the Cherry and Whites welcome Harlequins to Kingsholm in the Aviva Premiership on Saturday (kick off 3pm).
The Early Bird deadline for ticket purchases for the Aviva Premiership fixture against Harlequins on Saturday will expire at 6:30pm on Thursday evening, with tickets already limited for one area of Kingsholm.