Head coach Mark Mapletoft's side face Wales in the opening round at Gloucester this Friday night (7.45pm).
Lock James Gaskell (Sale Sharks), full back Tom Homer (London Irish) and fly half Rory Clegg (Harlequins) return from first team duty in the Heineken Cup and Amlin Cup.
All three started last summer's IRB Junior World Championship final against New Zealand in Tokyo but missed last week's 61-7 warm-up win against Cambridge University, when England ran in nine tries against the students.
Several players were involved in LV= Cup games on Saturday and Sunday, shortening England's preparation time for the game at Kingsholm.
Mapletoft said:
"We feel we've got our strongest available squad, which is always positive, because we know what a tough contest Wales will be for us."
"Fourteen of the 26 have been capped at this level before and that experience will be invaluable."
"Personally, as a player and a coach, games against Wales are always the ones I look forward to most eagerly. The rivalry always gets the pulses racing, particularly down at Kingsholm, and I think it'll be a special occasion."
"The five day turnaround will be good experience for the players and if we get our heads down and work hard everything else will look after itself."
Six players from the 32-man Under 20 Elite Player Squad miss out.
Of the backs, Christian Wade (London Wasps) is with the England Sevens squad, Jordi Pasqualin (Gloucester Rugby) and Marcus Watson (London Irish) are injured, and forwards Mako Vunipola (Bristol Rugby), Kieran Brookes (Newcastle Falcons) and Peter Elder (London Wasps) stand down.
Forwards
Joe Marler (Harlequins), Lee Imiolek (Sale Sharks), Jamie George (Saracens), Arthur Ellis (London Wasps), Shaun Knight (Gloucester Rugby), Calum Green (Leicester Tigers), Danny Wright (Gloucester Rugby), James Gaskell (Sale Sharks), Charlie Matthews (Harlequins), Jamie Gibson (London Irish), Jacob Rowan (Leeds Carnegie), Will Welch (Newcastle Falcons), Alex Gray (Newcastle Falcons), Jackson Wray (Saracens)
Backs
Sam Harrison (Leicester Tigers), Sam Stuart (Harlequins), Freddie Burns (Gloucester Rugby), Rory Clegg (Harlequins), Jake Sharp (Saracens), Tom Casson (Saracens), Tom Catterick (Newcastle Falcons), Jonny May (Gloucester Rugby), Jonathan Joseph (London Irish), Sam Smith (Harlequins), Will Hurrell (Leicester Tigers), Tom Homer (London Irish)
England's RBS U20 Six Nations fixtures (local times)
Feb 5: England U20 v Wales U20 (Gloucester, 7.45pm) live on Sky Sports
Feb 12: Italy U20 v England U20 (San Dona di Piave, 7pm)
Feb 26: England U20 v Ireland U20 (Gloucester, 7.45pm)
Mar 12: Scotland U20 v England U20 (Firhill, 7.30pm)
Mar 21: France U20 v England U20 (St Nazaire, 3pm)
Tickets are still available and priced as follows:
Seats in the C&G and JS Stands: Adult £15, Concession £12 and Juniors £5.
Standing in the Shed and West Terrace: Adult £10, Concession £8 and Juniors £4.
Gloucester Rugby season ticket holders will be able to purchase tickets at the following discounted prices:
Adult £13, Concession £10 and Juniors £3 in the C&G and JS Stands.
Adult £8, Concession £6 and Juniors £2 in the Shed and West Terrace.
Supporters can also save money by booking the double match package priced as follows:
Adult £26, Concessions £20 and Juniors £6 in the C&G and JS Stands.
Adults £16, Concession £12 and Juniors £4 in the Shed and West Terrace.
A number of hospitality packages are still available for both matches in the C&G Stand and West Stand. Prices start from £500 plus VAT for a 12 person suite.
For more information call the Commerical sales team on 0871 871 8781 option 4 or email sales@gloucesterrugby.co.uk
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