The entire Gloucester squad took part in the event to raise money for vulnerable people in the UK and in the poorest countries around the world.
The route was two laps of Archdeacon Meadow and the squad was split into two groups that went off at 11am and 12pm surrounded by thousands of runners, Gloucester supporters, children and adults.
"We were hoping for a really good turnout and that is exactly what we got," Little told www.gloucesterrugby.co.uk "A huge thank you must go to King's School who stepped in at the last minute and helped us run the event.
"Gloucester Rugby is a club at the heart of the community and that is why we wanted to get involved in this national event. The support we received from the playing squad was fantastic - they really got into the spirit of the event and it was great they were able to take part.
"To see supporters mix alongside stars like Mike Tindall and Jake Boer just emphasised how important Gloucester is to the community and that is what made the event such a success.
"I think 12 or 13 million pounds has been raised nationally and Gloucester Rugby played a part in that."
Milers were able to get their faces painted, take part in kicking competitions and gather autographs as the players mingled with the crowds.
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