And the event has been backed by the senior Gloucester squad and coaching staff, who will run the mile alongside supporters to make the day one of biggest in the west country.
"We had obviously hoped Sport Relief and the Open Day would take place at Kingsholm, but it is fantastic that King's School have stepped in to ensure the event will go ahead," said Gloucester's community development manager Gary Little.
"And now the playing squad has backed the event, it should be a massive occasion. To have the chance to run a mile alongside Jake Boer and Olly Morgan - if you can keep up - does not come around too often and we obviously hope it will be a huge draw to the event.
"It is great that Dean Ryan and his squad want to get involved in this nationwide event and we believe it will be a huge success."
The event will be split into two starting times - at 11am and 12pm - and the majority of the senior playing squad will be split into two groups to complete the course.
There will also be bouncy castles and the European Challenge Cup will also be on display as Gloucester aim to host one of the biggest events in the west.
Head coach Dean Ryan told www.gloucesterrugby.co.uk "Sport Relief is a nation-wide event and if we can support in some small way then I think it is fantastic. A lot of hard work has gone into making the event a success and we are right behind it.
"I am just glad the event can still go on as planned and we are all looking forward to it."
The entire country, including celebrities, politicians and the general public are urged to do a mile to raise money to help vulnerable people leading tough lives here in the UK, and in the poorest countries of the world.
The Gloucester Rugby Sport Relief Mile is just one of hundreds of mile events that will be taking place on Sport Relief Saturday. These events will be open to the public to ensure that the whole of the UK can Go the Extra Mile for Sport Relief.
The Sport Relief Red Sock is central to the experience of everyone taking part in the day. Each 'miler' will take part wearing one red sock, and at the finish line they'll be presented with a second to make a pair, and show the world that they've socked it to poverty.
All those people who have already registered, milers will receive information about the new start times as soon as possible. If there are any concerns, please contact Gary Little on 01452 300951.
To book, contact www.sportrelief.com