The brilliant England skipper led from the front with a crucial individual try as the Kingsholm club overcame the threat of a hugely physical Wasps side 35-26 in a thrilling finale to land the silverware.
After the heart-break of losing out in the final to the British Army last season, Gloucester exacted sweet revenge by triumphing in the showpiece event.
The Gloucester squad celebrate the Compass Group Middlesex Sevens title
Caption: Simon Amor takes the trophy.
A delighted Amor told www.gloucesterrugby.co.uk immediately after the final whistle from the dressing room: "I am really, really pleased. We only had a short session on Thursday to pull it all together so it was very much just turn up and play.
"I think the key to it all was that we played well together as a squad and the young guys did superbly. I am just really proud to have won it for Gloucester."
Talking of the key moment in Gloucester's run to the final, Amor added: "Against the Army we defended non-stop for a good number of minutes and our 'D' was very special - which is what we wanted to base our game on.
"The team spirit in general reflects on what is going on as a whole at Kingsholm at the moment. Everyone is working hard for each other and everyone wants to achieve something."
Caption: Anthony Allen in action against Wasps in the final
Mark Hewitt Gloucester's coach added: "One of the key aspects of our plan was to come and work hard on defence. We knew that if we could win turn-over ball then we would score tries.
"We kept telling each other that when we got the ball we had to keep hold of it and inject the pace. Simon Amor kept the team together in the middle and his communication skills are second to none.
"Our defence was brilliant and with Amor's control and everybody else stepping up to the mark it allowed our sweep defence to do the job. From what I understand, it's the first time we have won the title and it's fantastic."
Caption: James Bailey celebrates with the trophy
In the first game of the tournament, they overcame a spirited Worcester side thanks to a last minute try from Anthony Allen, who would go on to become one of the major stars of the tournament.
The other try scorers were James Bailey, who crossed twice, and Amor in a victory that set up a mouth-watering return in the quarter-final against the Army side.
But Amor's boys were not to be denied. Bailey was the hero with a try double after Olly Morgan reduced a 12-0 deficit with the first Gloucester score.
In the semi-final, Amor and Luke Narraway crossed for scores to settle a tense encounter against Harlequins but it seemed destined Gloucester would meet Wasps - the other outstanding side - in the final.
Caption: James Bailey hits the try line against Wasps in the final.
The final was a superb contest with Gloucester's off-the-cuff running style against Wasps' superior power but once they overcame a dodgy start, Amor's men were not to be denied.
Allen, surely a man with a huge Premiership future, started Gloucester's effort with two tries, Bailey, an international with blistering sevens pace, Amor and a penalty try sealed a 35-26 victory for Gloucester.
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