Henry Paul will miss the remainder of the season after Gloucester's medical team confirmed he has damaged the medial ligament in his knee.
The England international injured his knee playing for England Sevens at the World Cup in Hong Kong 10 days ago and was ruled out of last Saturday's win over London Irish.
Paul is set to be out for between six and eight weeks and will be unavailable for Gloucester's final three Zurich Premiership games, and any play-off matches.
Commenting on www.gloucesterrugbyclub.com Melville said: "It's a set-back but in some ways sums up our season. We have just had so many people injured at crucial periods, but you only have to look around the Premiership to see that other clubs are in a similar situation."
Commenting on www.gloucesterrugbyclub.com Paul added: "It's a big disappointment. I'm concentrating on getting back to full fitness, and will be right behind the rest of the team as we push for the top three slot in the Premiership and next season's Heineken Cup."
Paul, who has missed only a handful of games in four seasons with Gloucester, will see a specialist next week who will assess whether an operation is required.
Despite the injury, Nigel Melville's fly-half options could be boosted for Gloucester's next Premiership game at home to Leeds Tykes on Saturday 9 April with the return of Duncan McRae.
The Aussie stand-off has had an injury hit season, with his most recent knee injury ruling him out since the defeat away at Quins at the beginning of February.
As well as McRae's possible return, 22 year-old Brad Davies, who played the full 80 minutes at number 10 for Gloucester in the 13-12 win over London Irish on Saturday, was in commanding form for Gloucester against the Exiles at the weekend.
Gloucester Rugby CEO Stephen Vaughan was delighted to introduce ADEY Professional Heating Solutions as the club's new main sponsor this week. He spoke to the club website about the exciting new partnership.
Although understandably disappointed following the 40-39 defeat at Exeter, Freddie Burns thought that both teams fully contributed to a great spectacle but that a couple of refereeing decisions were key to the outcome.
Gloucester's final game of the season ended in a narrow defeat in a pulsating encounter at the Exeter Chiefs. The Cherry and Whites went down 40-39, as Gareth Steenson's dramatic late penalty meant that the end of Director of Rugby Nigel Davies' first season in charge ended in defeat. Davies spoke to the official website in the Devon sunshine.
Dave Lewis was in a tough situation on Saturday afternoon - playing for Gloucester against the side he will join next season, Exeter Chiefs. At the final whistle though, Lewis was simply disappointed to have lost the game.
Tongan second row Lua Lokotui is looking forward to catching up with some old acquaintances at Sandy Park when Gloucester take on Exeter Chiefs on Saturday afternoon, as he and his team mates hope to finish the season on a high note.
During a break from training ahead of Saturday's Aviva Premiership fixture at Exeter, centre Henry Trinder hopes that Gloucester build on the momentum from the recent win over Saracens, and expressed frustration that he's hitting form as the season end approaches after missing time due to injury.
Gloucester Rugby may have already clinched their Heineken Cup spot for 2013-14, but Director of Rugby Nigel Davies stressed this week that it's important for Gloucester to finish their season on a positive note at Exeter on Saturday.
Gloucester Rugby CEO Stephen Vaughan spoke to the Official Site this afternoon after he revealed the great news that Kingsholm Stadium was to play host to 4 pool matches of the Rugby World Cup 2015. He spoke of how much it means to the club as a whole and that all the hard work put in with Gloucester's bid had been rewarded.
On an exciting day for both Gloucester Rugby and the City, the Cherry and Whites' own World Cup Winner Jimmy Cowan spoke to the Official Site expressing his delight that Kingsholm had been chosen as a Host City and Venue for the Rugby World Cup 2015.
Anyone who has visited Kingsholm Stadium in the past couple of weeks will have noticed that the pitch isn't quite looking as it normally does. Stadium Manager Dave Balmer explained what work is currently taking place.