Henry Paul will miss the remainder of the season after Gloucester's medical team confirmed he has damaged the medial ligament in his knee.
The England international injured his knee playing for England Sevens at the World Cup in Hong Kong 10 days ago and was ruled out of last Saturday's win over London Irish.
Paul is set to be out for between six and eight weeks and will be unavailable for Gloucester's final three Zurich Premiership games, and any play-off matches.
Commenting on www.gloucesterrugbyclub.com Melville said: "It's a set-back but in some ways sums up our season. We have just had so many people injured at crucial periods, but you only have to look around the Premiership to see that other clubs are in a similar situation."
Commenting on www.gloucesterrugbyclub.com Paul added: "It's a big disappointment. I'm concentrating on getting back to full fitness, and will be right behind the rest of the team as we push for the top three slot in the Premiership and next season's Heineken Cup."
Paul, who has missed only a handful of games in four seasons with Gloucester, will see a specialist next week who will assess whether an operation is required.
Despite the injury, Nigel Melville's fly-half options could be boosted for Gloucester's next Premiership game at home to Leeds Tykes on Saturday 9 April with the return of Duncan McRae.
The Aussie stand-off has had an injury hit season, with his most recent knee injury ruling him out since the defeat away at Quins at the beginning of February.
As well as McRae's possible return, 22 year-old Brad Davies, who played the full 80 minutes at number 10 for Gloucester in the 13-12 win over London Irish on Saturday, was in commanding form for Gloucester against the Exiles at the weekend.
After a longer than usual preseason, Backs & Attack Coach Nick Walshe told GRTV this week that everyone at the club is looking forward to the trip this weekend to take on Exeter Chiefs in the opening round of the West Country Challenge Cup.
Gloucester Rugby hosted their first ever Beach Weekender over the Bank Holiday, and Commercial Director Mike Turner hailed the event as a great success as supporters braved the weather to flock to Gloucester Quays for a weekend of fun and rugby on the beach.
Speaking after his team's exit in the semi-finals, following a 19-14 defeat to the eventual winners the Newport Gwent Dragons, Coach Tim Taylor spoke to GRTV of how pleased he was with how his team acquitted themselves at the Singha Premiership Rugby 7s Finals.
Ben Vellacott was one of the standout performers in last week's pool stages and, ahead of Friday evening's Singha Premiership Rugby 7s Finals, told GRTV that he and his team mates will just try and play their own game once again.
Gloucester Rugby number eight Ben Morgan was today named in England's squad for Rugby World Cup 2015, a reward for eight months of hard work recuperating from a serious injury suffered back in January. Ben was one of a number of players who spoke at a press conference following the squad announcement.
Steph Reynolds led from the front once again as Gloucester kicked off their Singha Premiership Rugby 7s campaign in style on Thursday evening. The flyer scored three tries but admitted that he left most of the team talk duties to others.
Olly Morgan led the Cherry and Whites to three wins out of three in Group B of the Singha Premiership Rugby 7s and he expressed his delight in the young members of the squad in excelling in front of a bumper Kingsholm crowd.
Olly Morgan leads the Gloucester Rugby Sevens squad this Thursday in the Singha Premiership Rugby 7s Series at Kingsholm, and he is backing the young side to take the opportunity with both hands as they take to the field.
The Cherry and Whites kick off the inaugural West Country Challenge Cup at Sandy Park this Saturday when they meet Exeter Chiefs (KO 4:30), and front rower Dan Murphy is delighted that the return to action has arrived.
Rugby World Cup is approaching on the horizon, and with many of the Rugby World's most decorated and colourful stars set to descend on Kingsholm Stadium for the tournament, we gauged the mood in the camps as the preparations intensify.