Henry Paul will miss the remainder of the season after Gloucester's medical team confirmed he has damaged the medial ligament in his knee.
The England international injured his knee playing for England Sevens at the World Cup in Hong Kong 10 days ago and was ruled out of last Saturday's win over London Irish.
Paul is set to be out for between six and eight weeks and will be unavailable for Gloucester's final three Zurich Premiership games, and any play-off matches.
Commenting on www.gloucesterrugbyclub.com Melville said: "It's a set-back but in some ways sums up our season. We have just had so many people injured at crucial periods, but you only have to look around the Premiership to see that other clubs are in a similar situation."
Commenting on www.gloucesterrugbyclub.com Paul added: "It's a big disappointment. I'm concentrating on getting back to full fitness, and will be right behind the rest of the team as we push for the top three slot in the Premiership and next season's Heineken Cup."
Paul, who has missed only a handful of games in four seasons with Gloucester, will see a specialist next week who will assess whether an operation is required.
Despite the injury, Nigel Melville's fly-half options could be boosted for Gloucester's next Premiership game at home to Leeds Tykes on Saturday 9 April with the return of Duncan McRae.
The Aussie stand-off has had an injury hit season, with his most recent knee injury ruling him out since the defeat away at Quins at the beginning of February.
As well as McRae's possible return, 22 year-old Brad Davies, who played the full 80 minutes at number 10 for Gloucester in the 13-12 win over London Irish on Saturday, was in commanding form for Gloucester against the Exiles at the weekend.
Speaking after the game, Charlie Sharples described it as 'the win that has eluded us.' The winger stressed that Gloucester had stayed positive after the first couple of weeks of the Aviva Premiership season and the 26-13 victory over Sale was a result of that focus.
Gloucester Rugby picked up their first win of the new Aviva Premiership season on Friday night, easing to a 26-13 win on the road at Sale Sharks. Director of Rugby David Humphreys was full of praise for his players on the night.
Defence Coach Jonny Bell was on media duties ahead of the Aviva Premiership fixture at Sale Sharks on Friday evening, and spoke about where Gloucester find themselves in terms of their form at the moment as well as how he's viewing their defensive displays so far.
Callum Braley, who skippered Gloucester United to a 22-19 Aviva Premiership A League win over Saracens Storm on Monday evening, paid tribute to his forwards and the younger players in what he described as not the prettiest of wins.
Wednesday evening saw the launch of the Cherry and White Wednesday show on the Drivetime programme on BBC Radio Gloucestershire, with Henry Trinder live in the studio to kick off a regular weekly slot.
Gloucester Wheelchair Rugby Club hosted their second invitational tournament on Sunday 18th September. Teams from the South Wales Ospreys, the Southampton Sharks, Stoke Mandeville Maulers and the West Country Hawks travelled to Leisure @ in Cheltenham and played a 'round robin' sequence of games.
Kingsholm will play host to the inaugural Mitsubishi Motors Hybrid Cup on Sunday 9 October (kick off 3pm), where the Gloucester Rugby Heroes meet the Salford Red Devils Legends in a cross-code exhibition clash with legends of both games set to grace the Kingsholm turf again.