Henry Paul will miss the remainder of the season after Gloucester's medical team confirmed he has damaged the medial ligament in his knee.
The England international injured his knee playing for England Sevens at the World Cup in Hong Kong 10 days ago and was ruled out of last Saturday's win over London Irish.
Paul is set to be out for between six and eight weeks and will be unavailable for Gloucester's final three Zurich Premiership games, and any play-off matches.
Commenting on www.gloucesterrugbyclub.com Melville said: "It's a set-back but in some ways sums up our season. We have just had so many people injured at crucial periods, but you only have to look around the Premiership to see that other clubs are in a similar situation."
Commenting on www.gloucesterrugbyclub.com Paul added: "It's a big disappointment. I'm concentrating on getting back to full fitness, and will be right behind the rest of the team as we push for the top three slot in the Premiership and next season's Heineken Cup."
Paul, who has missed only a handful of games in four seasons with Gloucester, will see a specialist next week who will assess whether an operation is required.
Despite the injury, Nigel Melville's fly-half options could be boosted for Gloucester's next Premiership game at home to Leeds Tykes on Saturday 9 April with the return of Duncan McRae.
The Aussie stand-off has had an injury hit season, with his most recent knee injury ruling him out since the defeat away at Quins at the beginning of February.
As well as McRae's possible return, 22 year-old Brad Davies, who played the full 80 minutes at number 10 for Gloucester in the 13-12 win over London Irish on Saturday, was in commanding form for Gloucester against the Exiles at the weekend.
Named Man of the Match by BT Sport, scrum-half Willi Heinz said after the 15-11 win at Bath on Friday evening that it was a pretty happy Gloucester dressing room and reward for a lot of hard work put in during the week leading up to the game.
Speaking after the 15-11 win over Bath at the Rec on Friday evening, David Humphreys heaped praise on his players, pointing out how they had bounced back after last week's tough defeat to overcome a rough start before controlling the game and earning the win.
Gloucester Rugby hooker Richard Hibbard spoke to the local media this week as the Cherry and Whites prepare to face Bath Rugby in the Aviva Premiership. The Welshman is ready to bounce back at The Rec.
Speaking to the local media about the local derby against Bath at the Rec on Friday evening, Director of Rugby David Humphreys acknowledged that it will be a tough encounter but hugely exciting at the same time and one the squad are looking forward to.
A visibly disappointed Billy Twelvetrees admitted that it was a very sombre Gloucester dressing room as a last minute try and conversion condemned the Cherry and Whites to an 18-19 defeat against Leicester Tigers on Saturday.
A disappointed Tom Savage told GRTV, after the 18-19 defeat at home to the Leicester Tigers, that the whole Gloucester team had put in a fantastic effort, but just couldn't find the extra one or two percent necessary to put away a top side.
Speaking after the narrow loss to Leicester Tigers on Saturday afternoon, David Humphreys says despite there being a lot of good in Gloucester's performance, he rued the failure to close the game out, as well as thanking the home support
Kiwi second row Jeremy Thrush is thoroughly enjoying his time in Cherry and White so far, and this weekend the Leicester Tigers are the visitors to Kingsholm, and the All Black is massively excited about the challenge.
After two wins in the European Rugby Challenge Cup, securing a home quarter-final, David Humphreys told the local media this week that Gloucester are coming into Saturday's game in good physical shape having rotated the squad. However, he and the squad are prepared for a tough encounter against the Tigers.
It's almost four years since Gloucester won a Premiership game at the Rec against Bath. However, they got that monkey off their back on Friday evening with a hard-fought 15-11 win in testing conditions.