The event takes place at 7.30pm on Tuesday 15 February in the bar area of the main grandstand at Kingsholm.
The evening will see Gloucester players team up with other names from Gloucestershire sport and battle it out for the title of Question of Sport champions.
BBC Radio Gloucestershire's Steve Kitchen will host the evening, and the recording of the show will be broadcast on the radio sometime soon after.
Tickets are £10 and all proceeds will go to the two charities. There will also be an auction and raffle. Last year's event was a big success and a great evening out.
Tickets can be purchase from the Kingsholm ticket office on 0871 871 8781, or supporters can pay on the evening.
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