Two members of Gloucester's Zurich 'A' League team this season have been included in the England Students squad to play France on Friday.
Wing/centre Mark Foster and second row Tom Skelding, who both attend Gloucester's Hartpury College are named in the squad alongside former Academy prop Mark Irish.
Brad Davies misses out after being involved in the last game, as he's required for Sunday's Zurich Premiership clash away at Newcastle.
New additions to the squad include Bath wing Owen Evans, Exeter fly-half Adam Staniforth, Bristol prop Simon Carter and Durham hooker Robert Vickers.
Loughborough prop Dave Bassett travels as cover for Mark Irish, who will have a fitness test on Thursday.
Evans replaces injured Oxford wing Jonan Boto, while prop George Davis and hooker Robin Scothern are unavailable and fly-half Bradley Davies is on Zurich Premiership duty with Gloucester.
England beat the French 10-6 at Blackheath earlier this month thanks to a penalty try and a conversion and penalty from Oxford centre James Whittingham.
But they are expecting a backlash at La Roche Sur Yon - midway between Nantes and La Rochelle - when they meet again on Friday night (7.30pm local time).
"We've done well so far but it'll be interested to see what changes they've made for the return game and we're expecting them to come back at us," said manager Tony Lanaway.
England Students are in action again next month when they take on Italy at Iffley Road, Oxford, on Friday March 11 (6pm).
England Students squad Justin Abrahams (Loughborough)
Dave Bassett (Loughborough)
Simon Carter (Bristol)
Andy Dalgleish (Oxford)
Owen Evans (Bath)
Mark Foster (Hartpury Coll UWE)
Simon Gibbons (Exeter)
Sacha Harding (De Montfort)
Adam Harris (Oxford)
Tom Hayman (Oxford)
Andrew Houston (Loughborough)
Mark Irish (Hartpury Coll UWE)
Ali James (Oxford)
Chris Lowrie (College of St Mark & St John, Plymouth)
Luke Sayer (Nottingham)
Tom Skelding (Hartpury Coll UWE)
Adam Staniforth (Exeter)
Haydn Thomas (Exeter)
Wayne Thompson (Bristol)
Robert Vickers (Durham)
Ben Wheeler (Cambridge)
James Whittingham (Oxford)
Chris Wyles (Nottingham)
Speaking to the local media this week, John Afoa described the hard work that the Cherry and White have been putting in and that he is fighting fit ready for Saturday's European Rugby Challenge Cup semi final clash with Exeter Chiefs.
Gloucester Rugby face Exeter Chiefs this Saturday in the European Rugby Challenge Cup semi-final, and Charlie Sharples, who starts at full back is relishing the challenge of the big Kingsholm occasion.
Gloucester meet the Exeter Chiefs for the fourth time in competitive action this weekend, this time in the semi-final of the European Rugby Challenge Cup. Director of Rugby David Humphreys told the local media this week that this is the sort of game that Gloucester want to be involved in on a regular basis and that he and his team are looking forward to it.
Following the 29-26 loss to Harlequins, club captain Billy Twelvetrees spoke of his disappointment that the Cherry and Whites were unable to cash in on the home side's indiscipline and lose a lead on Saturday afternoon.
Number eight Gareth Evans admitted to a collective feeling of frustration as Gloucester lost narrowly at Harlequins on Saturday, but insisted that there are plenty of positives to draw upon ahead of next Saturday's European Rugby Challenge Cup semi-final at home to Exeter.
Gloucester travel to the Stoop on Saturday afternoon to take on Harlequins in the Aviva Premiership. The Cherry and Whites make just two changes from last weekend's win over Connacht as they seek consistency and continuity in their play as Nick Walshe told the local media this week.
The Gloucester Rugby Ticket office can advise that the club's initial allocation of tickets for the European Rugby Challenge Cup Final has sold out. However, the club has secured an additional allocation of tickets from EPCR which will go on sale on Monday.
Following Saturday evening's exciting European Rugby Challenge Cup semi-final victory over Exeter Chiefs, the Ticket Office at Kingsholm will be open on Sunday from 11am to 3pm for Club Members to purchase tickets for the Final.
Gloucester Rugby qualified for their first European final since 2006 as they defeated the in-form Exeter Chiefs by 30 points to 19 in a thrilling semi-final at Kingsholm on Saturday evening. The club photographer caught the best of the action.
Recent meetings between these two teams have often been thrillers, and this was no exception. It went down to the final few minutes, but Gloucester produced the goods when it mattered to qualify for the European Rugby Challenge Cup Final, beating Exeter 30-19.