Two members of Gloucester's Zurich 'A' League team this season have been included in the England Students squad to play France on Friday.
Wing/centre Mark Foster and second row Tom Skelding, who both attend Gloucester's Hartpury College are named in the squad alongside former Academy prop Mark Irish.
Brad Davies misses out after being involved in the last game, as he's required for Sunday's Zurich Premiership clash away at Newcastle.
New additions to the squad include Bath wing Owen Evans, Exeter fly-half Adam Staniforth, Bristol prop Simon Carter and Durham hooker Robert Vickers.
Loughborough prop Dave Bassett travels as cover for Mark Irish, who will have a fitness test on Thursday.
Evans replaces injured Oxford wing Jonan Boto, while prop George Davis and hooker Robin Scothern are unavailable and fly-half Bradley Davies is on Zurich Premiership duty with Gloucester.
England beat the French 10-6 at Blackheath earlier this month thanks to a penalty try and a conversion and penalty from Oxford centre James Whittingham.
But they are expecting a backlash at La Roche Sur Yon - midway between Nantes and La Rochelle - when they meet again on Friday night (7.30pm local time).
"We've done well so far but it'll be interested to see what changes they've made for the return game and we're expecting them to come back at us," said manager Tony Lanaway.
England Students are in action again next month when they take on Italy at Iffley Road, Oxford, on Friday March 11 (6pm).
England Students squad Justin Abrahams (Loughborough)
Dave Bassett (Loughborough)
Simon Carter (Bristol)
Andy Dalgleish (Oxford)
Owen Evans (Bath)
Mark Foster (Hartpury Coll UWE)
Simon Gibbons (Exeter)
Sacha Harding (De Montfort)
Adam Harris (Oxford)
Tom Hayman (Oxford)
Andrew Houston (Loughborough)
Mark Irish (Hartpury Coll UWE)
Ali James (Oxford)
Chris Lowrie (College of St Mark & St John, Plymouth)
Luke Sayer (Nottingham)
Tom Skelding (Hartpury Coll UWE)
Adam Staniforth (Exeter)
Haydn Thomas (Exeter)
Wayne Thompson (Bristol)
Robert Vickers (Durham)
Ben Wheeler (Cambridge)
James Whittingham (Oxford)
Chris Wyles (Nottingham)
Speaking to the local media this week, Richard Hibbard expressed his excitement ahead of the West Country derby and that there is an extra spring in the step of the Cherry and Whites ahead of the tough challenge.
With such a short turnaround between the two games, and Gloucester looking to reintegrate several players into the line-up after the Autumn international series, Tom Savage told GRTV that the Cherry and Whites will keep changes to a minimum for the European Rugby Challenge Cup game in Zebre on Saturday.
Gloucester travel to Italy this weekend to take on Zebre in Round Four of the European Rugby Challenge Cup. The Cherry and Whites defeated the Italian side 35-10 last Sunday, but Director of Rugby David Humphreys is looking for an improved performance in the return fixture.
Skipper Billy Twelvetrees, speaking to GRTV after the 35-10 over Zebre, was happy with the bonus point win over the Italian side but admitted Gloucester will need to tighten up for the return fixture in Parma.
Gloucester Rugby welcome Sione Kalamafoni, Greig Laidlaw and Tom Palmer back to the starting line up and feature David Halaifonua in a matchday squad for the first time, as they resume Aviva Premiership action against Bath Rugby at Kingsholm on Saturday (kick off 5:30pm).