Two members of Gloucester's Zurich 'A' League team this season have been included in the England Students squad to play France on Friday.
Wing/centre Mark Foster and second row Tom Skelding, who both attend Gloucester's Hartpury College are named in the squad alongside former Academy prop Mark Irish.
Brad Davies misses out after being involved in the last game, as he's required for Sunday's Zurich Premiership clash away at Newcastle.
New additions to the squad include Bath wing Owen Evans, Exeter fly-half Adam Staniforth, Bristol prop Simon Carter and Durham hooker Robert Vickers.
Loughborough prop Dave Bassett travels as cover for Mark Irish, who will have a fitness test on Thursday.
Evans replaces injured Oxford wing Jonan Boto, while prop George Davis and hooker Robin Scothern are unavailable and fly-half Bradley Davies is on Zurich Premiership duty with Gloucester.
England beat the French 10-6 at Blackheath earlier this month thanks to a penalty try and a conversion and penalty from Oxford centre James Whittingham.
But they are expecting a backlash at La Roche Sur Yon - midway between Nantes and La Rochelle - when they meet again on Friday night (7.30pm local time).
"We've done well so far but it'll be interested to see what changes they've made for the return game and we're expecting them to come back at us," said manager Tony Lanaway.
England Students are in action again next month when they take on Italy at Iffley Road, Oxford, on Friday March 11 (6pm).
England Students squad Justin Abrahams (Loughborough)
Dave Bassett (Loughborough)
Simon Carter (Bristol)
Andy Dalgleish (Oxford)
Owen Evans (Bath)
Mark Foster (Hartpury Coll UWE)
Simon Gibbons (Exeter)
Sacha Harding (De Montfort)
Adam Harris (Oxford)
Tom Hayman (Oxford)
Andrew Houston (Loughborough)
Mark Irish (Hartpury Coll UWE)
Ali James (Oxford)
Chris Lowrie (College of St Mark & St John, Plymouth)
Luke Sayer (Nottingham)
Tom Skelding (Hartpury Coll UWE)
Adam Staniforth (Exeter)
Haydn Thomas (Exeter)
Wayne Thompson (Bristol)
Robert Vickers (Durham)
Ben Wheeler (Cambridge)
James Whittingham (Oxford)
Chris Wyles (Nottingham)
Defence Coach Jonny Bell admitted this week that the European Rugby Challenge Cup victory in Bayonne was a welcome one, stressed that Benetton Treviso will pose a tough test on Saturday at Kingsholm but that Gloucester are determined to deliver in front of their own fans.
Motu Matu'u is looking to make up for lost time this week as the Cherry and Whites head to Basque Country to take on Bayonne in the European Rugby Challenge Cup opener. The Samoan hooker made his first appearance in the jersey last weekend, and is hoping to contribute to the team this weekend.
Gloucester's attentions turn to Europe this weekend as they travel to Bayonne for their opening fixture of this season's European Rugby Challenge Cup. Forwards Coach Trevor Woodman told GRTV that the Cherry and Whites will be aiming to top their pool as they've done for the past two seasons, but is well aware of how tough it is to win in France.
Although understandably disappointed at seeing victory slip away at the last minute, Ben Morgan said after the game at Exeter that there were plenty of positives that Gloucester could take from the game.
Speaking after a last-gasp try from Exeter earned the Chiefs a 27-27 draw at Sandy Park on Saturday, Charlie Sharples admitted to mixed feelings. The game saw him score a try on his 200th competitive appearance for the club, but the frustration from not closing out the win was clear.
David Humphreys was minutes away from a winning interview on Saturday afternoon, but a late try from Exeter Chiefs meant the points were shared at Sandy Park. Humphreys spoke with the local media after the 27-27 draw.
Speaking to the media ahead of Saturday's Aviva Premiership fixture away at Exeter Chiefs, Director of Rugby David Humphreys reiterated that there are elements of Gloucester's game that are in good shape at present, that the performance against Bath was a step forward from Newcastle but that individual errors are costing the Cherry and Whites dear.
Centre Matt Scott, a try-scorer in Bayonne last weekend, insists that Gloucester are taking nothing for granted when they welcome Benetton Treviso to Kingsholm on Saturday in the European Rugby Challenge Cup (kick off 3pm).