Two members of Gloucester's Zurich 'A' League team this season have been included in the England Students squad to play France on Friday.
Wing/centre Mark Foster and second row Tom Skelding, who both attend Gloucester's Hartpury College are named in the squad alongside former Academy prop Mark Irish.
Brad Davies misses out after being involved in the last game, as he's required for Sunday's Zurich Premiership clash away at Newcastle.
New additions to the squad include Bath wing Owen Evans, Exeter fly-half Adam Staniforth, Bristol prop Simon Carter and Durham hooker Robert Vickers.
Loughborough prop Dave Bassett travels as cover for Mark Irish, who will have a fitness test on Thursday.
Evans replaces injured Oxford wing Jonan Boto, while prop George Davis and hooker Robin Scothern are unavailable and fly-half Bradley Davies is on Zurich Premiership duty with Gloucester.
England beat the French 10-6 at Blackheath earlier this month thanks to a penalty try and a conversion and penalty from Oxford centre James Whittingham.
But they are expecting a backlash at La Roche Sur Yon - midway between Nantes and La Rochelle - when they meet again on Friday night (7.30pm local time).
"We've done well so far but it'll be interested to see what changes they've made for the return game and we're expecting them to come back at us," said manager Tony Lanaway.
England Students are in action again next month when they take on Italy at Iffley Road, Oxford, on Friday March 11 (6pm).
England Students squad Justin Abrahams (Loughborough)
Dave Bassett (Loughborough)
Simon Carter (Bristol)
Andy Dalgleish (Oxford)
Owen Evans (Bath)
Mark Foster (Hartpury Coll UWE)
Simon Gibbons (Exeter)
Sacha Harding (De Montfort)
Adam Harris (Oxford)
Tom Hayman (Oxford)
Andrew Houston (Loughborough)
Mark Irish (Hartpury Coll UWE)
Ali James (Oxford)
Chris Lowrie (College of St Mark & St John, Plymouth)
Luke Sayer (Nottingham)
Tom Skelding (Hartpury Coll UWE)
Adam Staniforth (Exeter)
Haydn Thomas (Exeter)
Wayne Thompson (Bristol)
Robert Vickers (Durham)
Ben Wheeler (Cambridge)
James Whittingham (Oxford)
Chris Wyles (Nottingham)
Gloucester will field a reshuffled side against the Ospreys in the LV= Cup this weekend, but Backs & Attack Coach Nick Walshe stressed that it's a great opportunity for everyone on duty on Saturday and that maintaining the recent winning run is very important.
Skipper Billy Twelvetrees told GRTV after the win in Brive that the game could have been a tough one to get up for, but that his Gloucester team mates did themselves proud on the night and got a big result.
Reflecting on the 31-20 victory in Brive on Thursday evening, Director of Rugby David Humphreys said that clinching a home European Rugby Challenge Cup quarter-final was the overall aim and duly achieved.
Gloucester may have already clinched a home European Rugby Challenge Cup quarter-final, but Director of Rugby David Humphreys wants his side to finish the pool stages on a winning note in Brive on Thursday evening.
Speaking after Saturday's win, James Hudson was happy to record a bonus-point win against a hugely physical Oyonnax side as Gloucester secured their passage into the European Rugby Challenge Cup Quarter Finals
Billy Burns was a try scorer on his first European start for Gloucester and, speaking after the game, admitted that it was good to get the five points although there are still things to work on before the trip to Brive.
Gloucester Rugby have added to their front row strength in depth for next season with the signings of Paul Doran-Jones, Paddy McAllister and Nicky Thomas from Harlequins, Aurillac and the Ospreys respectively.