Two members of Gloucester's Zurich 'A' League team this season have been included in the England Students squad to play France on Friday.
Wing/centre Mark Foster and second row Tom Skelding, who both attend Gloucester's Hartpury College are named in the squad alongside former Academy prop Mark Irish.
Brad Davies misses out after being involved in the last game, as he's required for Sunday's Zurich Premiership clash away at Newcastle.
New additions to the squad include Bath wing Owen Evans, Exeter fly-half Adam Staniforth, Bristol prop Simon Carter and Durham hooker Robert Vickers.
Loughborough prop Dave Bassett travels as cover for Mark Irish, who will have a fitness test on Thursday.
Evans replaces injured Oxford wing Jonan Boto, while prop George Davis and hooker Robin Scothern are unavailable and fly-half Bradley Davies is on Zurich Premiership duty with Gloucester.
England beat the French 10-6 at Blackheath earlier this month thanks to a penalty try and a conversion and penalty from Oxford centre James Whittingham.
But they are expecting a backlash at La Roche Sur Yon - midway between Nantes and La Rochelle - when they meet again on Friday night (7.30pm local time).
"We've done well so far but it'll be interested to see what changes they've made for the return game and we're expecting them to come back at us," said manager Tony Lanaway.
England Students are in action again next month when they take on Italy at Iffley Road, Oxford, on Friday March 11 (6pm).
England Students squad Justin Abrahams (Loughborough)
Dave Bassett (Loughborough)
Simon Carter (Bristol)
Andy Dalgleish (Oxford)
Owen Evans (Bath)
Mark Foster (Hartpury Coll UWE)
Simon Gibbons (Exeter)
Sacha Harding (De Montfort)
Adam Harris (Oxford)
Tom Hayman (Oxford)
Andrew Houston (Loughborough)
Mark Irish (Hartpury Coll UWE)
Ali James (Oxford)
Chris Lowrie (College of St Mark & St John, Plymouth)
Luke Sayer (Nottingham)
Tom Skelding (Hartpury Coll UWE)
Adam Staniforth (Exeter)
Haydn Thomas (Exeter)
Wayne Thompson (Bristol)
Robert Vickers (Durham)
Ben Wheeler (Cambridge)
James Whittingham (Oxford)
Chris Wyles (Nottingham)
Gloucester travel to Oyonnax for the second round of the European Rugby Challenge Cup on Saturday evening, and Director of Rugby David Humphreys is expecting a big test from the French side, feeling that their league position doesn't reflect their recent performances.
Speaking exclusively to Gloucester Rugby TV, Matt Kvesic explained that his decision to extend his contract at Kingsholm was a simple one and that he was hugely excited for the future in Cherry and White.
Speaking after the 55-0 win over CA Brive, Jonny May told GRTV that he's delighted that the team's hard work is starting to pay dividends but was equally happy for his friend and team mate, Charlie Sharples, on what was a big night for his fellow winger.
Speaking to the local media this week, David Humphreys insisted that Gloucester are looking forward to kicking off the new European Rugby Challenge Cup against Brive on Thursday evening, although an unfamiliar opponent and a five day turnaround will pose a challenge.