Gloucester and Bath will meet in the semi-final of the Powergen Cup after the draw was made on Monday night.
The clash with Bath follows last weekend's West Country derby win over Bristol Shoguns in the quarter-final.
Commenting live on the radio after the draw Gloucester scrum-half Andy Gomarsall said: "I knew this was going to happen. We've just had one derby against Bristol so I thought another was on the cards.
"Derbies tend to bring out the best in us and the supporters. The atmosphere at Kingsholm against Bristol on Saturday was superb."
Gloucester clash with Bath in the Zurich Premiership at Kingsholm on Saturday 19 February in a 13,000 sell-out, and the Cup semi-final will take place on the weekend of the 5/6 March.
In the other semi-final Leeds, the only team in the draw not to have won the cup, and London Irish were paired in the other semi-final.
The Tykes' presence in the last four arrived courtesy of a 24-19 triumph over Northampton while Irish edged past Saracens 21-15 in a thrilling encounter at Vicarage Road yesterday.
Winger Justin Bishop, who was part of the Exiles side who claimed Powergen Cup glory in 2002, said: "This is a one-off game and hopefully we can get in the final again. That would be massive for our club.
"All the teams in the draw are quite similar at the moment - the big sides like Leicester and Wasps - so we weren't really bothered who we got."
Both matches will be played on the weekend of March 5-6 with the venues yet to be confirmed. Although, Gloucester were informed by the RFU, prior to the draw, that the first and third teams drawn from the hat would have home advantage.
Ticket details, and date and kick off times will be confirmed shortly.
Tom Marshall scored his first Gloucester try in the 31-23 win over Saracens on Friday evening and was named Man of the Match. Talking to GRTV, he praised the forwards for setting the tone on the night.
Matt Scott was on media duty this week, and the Scottish international spoke about the benefit of having a full week of training with his club mates and the determination to get back to performing well against Saracens this Friday evening.
After the disappointment of last weekend's performance at Leicester, Director of Rugby David Humphreys told GRTV that he expects a big response from Gloucester in Friday's Aviva Premiership fixture at home to Saracens (kick off 7:45pm).
Gloucester travel to Tigers this Saturday in good spirits, and fly half Billy Burns is looking forward to testing himself in one of the toughest environments in the league as the Cherry and White hunt vital points as attention returns to the Aviva Premiership
After a good run of recent results, Gloucester will travel to take on Leicester in a good frame of mind according to Director of Rugby David Humphreys, but are well aware of the challenge that lies in wait.
Mental health and wellbeing is just as important as physical health, which is why the Gloucester Rugby Community team have partnered with Public Health Gloucestershire to become Mental Health First Aiders.
Gloucester Rugby continues to strengthen its commitment to working across Gloucestershire and the surrounding area and is looking to recruit an enthusiastic, motivated and dedicated individual to the role of Wheelchair Rugby Coach
Wales have named their side to face Scotland at Murrayfield on Saturday. They make just one change as George North returns in place of Alex Cuthbert and Gloucester's Ross Moriarty starts again at number eight.