Gloucester and Bath will meet in the semi-final of the Powergen Cup after the draw was made on Monday night.
The clash with Bath follows last weekend's West Country derby win over Bristol Shoguns in the quarter-final.
Commenting live on the radio after the draw Gloucester scrum-half Andy Gomarsall said: "I knew this was going to happen. We've just had one derby against Bristol so I thought another was on the cards.
"Derbies tend to bring out the best in us and the supporters. The atmosphere at Kingsholm against Bristol on Saturday was superb."
Gloucester clash with Bath in the Zurich Premiership at Kingsholm on Saturday 19 February in a 13,000 sell-out, and the Cup semi-final will take place on the weekend of the 5/6 March.
In the other semi-final Leeds, the only team in the draw not to have won the cup, and London Irish were paired in the other semi-final.
The Tykes' presence in the last four arrived courtesy of a 24-19 triumph over Northampton while Irish edged past Saracens 21-15 in a thrilling encounter at Vicarage Road yesterday.
Winger Justin Bishop, who was part of the Exiles side who claimed Powergen Cup glory in 2002, said: "This is a one-off game and hopefully we can get in the final again. That would be massive for our club.
"All the teams in the draw are quite similar at the moment - the big sides like Leicester and Wasps - so we weren't really bothered who we got."
Both matches will be played on the weekend of March 5-6 with the venues yet to be confirmed. Although, Gloucester were informed by the RFU, prior to the draw, that the first and third teams drawn from the hat would have home advantage.
Ticket details, and date and kick off times will be confirmed shortly.
Following the 48-10 Aviva Premiership win over London Welsh on Saturday, Dan Murphy praised his team mates for battling right up to the very last minute in difficult conditions and maintaining the momentum that the team have built in 2015.
Henry Purdy scored two memorable tries against London Welsh at Kingsholm on Saturday, and attributed the first one to hard work on the training ground before describing how much he's enjoying playing his rugby alongside friends like Callum Braley.
Gloucester face London Welsh for the third time this season when they meet at Kingsholm in the Aviva Premiership on Saturday (kick off 3pm), but Director of Rugby David Humphreys insists his team are prepared for a tough game against the Exiles.
Billy Burns was a late call up to the Gloucester team that lost narrowly to the Leicester Tigers at Welford Road on Friday evening and, speaking to GRTV post match, was full of praise for his team-mates but admitted that defeat was tough to take.
Speaking after what he described as an 'arm wrestle' up front, skipper Tom Savage couldn't fault his side's effort and commitment on the night at Welford Road but insisted that his side will keep on battling, starting at home to London Welsh next weekend.
A number of the Gloucester Rugby squad this week benefitted from an initiative organised by Circle 2 Success (C2S) and the Rugby Players Association (RPA) to help players develop their business planning skills.