Gloucester and Bath will meet in the semi-final of the Powergen Cup after the draw was made on Monday night.
The clash with Bath follows last weekend's West Country derby win over Bristol Shoguns in the quarter-final.
Commenting live on the radio after the draw Gloucester scrum-half Andy Gomarsall said: "I knew this was going to happen. We've just had one derby against Bristol so I thought another was on the cards.
"Derbies tend to bring out the best in us and the supporters. The atmosphere at Kingsholm against Bristol on Saturday was superb."
Gloucester clash with Bath in the Zurich Premiership at Kingsholm on Saturday 19 February in a 13,000 sell-out, and the Cup semi-final will take place on the weekend of the 5/6 March.
In the other semi-final Leeds, the only team in the draw not to have won the cup, and London Irish were paired in the other semi-final.
The Tykes' presence in the last four arrived courtesy of a 24-19 triumph over Northampton while Irish edged past Saracens 21-15 in a thrilling encounter at Vicarage Road yesterday.
Winger Justin Bishop, who was part of the Exiles side who claimed Powergen Cup glory in 2002, said: "This is a one-off game and hopefully we can get in the final again. That would be massive for our club.
"All the teams in the draw are quite similar at the moment - the big sides like Leicester and Wasps - so we weren't really bothered who we got."
Both matches will be played on the weekend of March 5-6 with the venues yet to be confirmed. Although, Gloucester were informed by the RFU, prior to the draw, that the first and third teams drawn from the hat would have home advantage.
Ticket details, and date and kick off times will be confirmed shortly.
Billy Twelvetrees spoke to Gloucester Rugby TV following the 17-18 loss to Bath Rugby in Round Nineteen of Aviva Premiership Rugby. The Gloucester skipper said he was proud of the desire shown by his team at the end of a tough afternoon.
Nigel Davies spoke to Gloucester Rugby TV following the gutting 17-18 reverse to Bath on Saturday afternoon. The Director of Rugby expressed his pride at the efforts from his players and thanked the supporters for their incredible backing.
Nick Wood spoke to Gloucester Rugby TV this week ahead of Saturday's West Country derby clash with Bath Rugby. The front rower called on the squad to make a strong start and get the Kingsholm crowd behind them
Director of Rugby Nigel Davies admits that it's been a tough week at Gloucester Rugby following defeat at London Wasps last weekend, but that he's looking for his team to rise to the occasion for the huge game at home to Bath Rugby on Saturday.
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Nigel Davies spoke to Gloucester Rugby TV following the 36-24 defeat to London Wasps in the Amlin Challenge Cup Quarter Final. The Director of Rugby rued a lacklustre showing the first half despite a big second half display with ultimately came to nothing on a hugely frustrating night for the Cherry and Whites.
Nick Wood spoke to the assembled media following the vital 14-13 victory over Exeter Chiefs on Saturday. The front-rower express his delight at the performance of the front row and challenged his colleagues to push on from the display
Ahead of this weekend's trip to Twickenham for The Stinger, there are plenty of videos on the way to preview the fixture vs London Wasps, as well as video reaction and unique Go Pro videos from the last 7 days of action.
Academy back rower Sam Underhill is readying to lead England Under 18s into battle for the final of the FIRA/AER European championships, and took time out of training to chat with the club website from their base in Poland.