Gloucester and Bath will meet in the semi-final of the Powergen Cup after the draw was made on Monday night.
The clash with Bath follows last weekend's West Country derby win over Bristol Shoguns in the quarter-final.
Commenting live on the radio after the draw Gloucester scrum-half Andy Gomarsall said: "I knew this was going to happen. We've just had one derby against Bristol so I thought another was on the cards.
"Derbies tend to bring out the best in us and the supporters. The atmosphere at Kingsholm against Bristol on Saturday was superb."
Gloucester clash with Bath in the Zurich Premiership at Kingsholm on Saturday 19 February in a 13,000 sell-out, and the Cup semi-final will take place on the weekend of the 5/6 March.
In the other semi-final Leeds, the only team in the draw not to have won the cup, and London Irish were paired in the other semi-final.
The Tykes' presence in the last four arrived courtesy of a 24-19 triumph over Northampton while Irish edged past Saracens 21-15 in a thrilling encounter at Vicarage Road yesterday.
Winger Justin Bishop, who was part of the Exiles side who claimed Powergen Cup glory in 2002, said: "This is a one-off game and hopefully we can get in the final again. That would be massive for our club.
"All the teams in the draw are quite similar at the moment - the big sides like Leicester and Wasps - so we weren't really bothered who we got."
Both matches will be played on the weekend of March 5-6 with the venues yet to be confirmed. Although, Gloucester were informed by the RFU, prior to the draw, that the first and third teams drawn from the hat would have home advantage.
Ticket details, and date and kick off times will be confirmed shortly.
Ben Vellacott was one of the standout performers in last week's pool stages and, ahead of Friday evening's Singha Premiership Rugby 7s Finals, told GRTV that he and his team mates will just try and play their own game once again.
Gloucester Rugby number eight Ben Morgan was today named in England's squad for Rugby World Cup 2015, a reward for eight months of hard work recuperating from a serious injury suffered back in January. Ben was one of a number of players who spoke at a press conference following the squad announcement.
Steph Reynolds led from the front once again as Gloucester kicked off their Singha Premiership Rugby 7s campaign in style on Thursday evening. The flyer scored three tries but admitted that he left most of the team talk duties to others.
Olly Morgan led the Cherry and Whites to three wins out of three in Group B of the Singha Premiership Rugby 7s and he expressed his delight in the young members of the squad in excelling in front of a bumper Kingsholm crowd.
Olly Morgan leads the Gloucester Rugby Sevens squad this Thursday in the Singha Premiership Rugby 7s Series at Kingsholm, and he is backing the young side to take the opportunity with both hands as they take to the field.
Speaking to the local media this week, Henry Purdy explained the opportunity available for the Gloucester Rugby to lay down a marker in this summer's Singha Premiership Sevens Series, which kicks off at Kingsholm Stadium on Thursday 20 August.