Gloucester and Bath will meet in the semi-final of the Powergen Cup after the draw was made on Monday night.
The clash with Bath follows last weekend's West Country derby win over Bristol Shoguns in the quarter-final.
Commenting live on the radio after the draw Gloucester scrum-half Andy Gomarsall said: "I knew this was going to happen. We've just had one derby against Bristol so I thought another was on the cards.
"Derbies tend to bring out the best in us and the supporters. The atmosphere at Kingsholm against Bristol on Saturday was superb."
Gloucester clash with Bath in the Zurich Premiership at Kingsholm on Saturday 19 February in a 13,000 sell-out, and the Cup semi-final will take place on the weekend of the 5/6 March.
In the other semi-final Leeds, the only team in the draw not to have won the cup, and London Irish were paired in the other semi-final.
The Tykes' presence in the last four arrived courtesy of a 24-19 triumph over Northampton while Irish edged past Saracens 21-15 in a thrilling encounter at Vicarage Road yesterday.
Winger Justin Bishop, who was part of the Exiles side who claimed Powergen Cup glory in 2002, said: "This is a one-off game and hopefully we can get in the final again. That would be massive for our club.
"All the teams in the draw are quite similar at the moment - the big sides like Leicester and Wasps - so we weren't really bothered who we got."
Both matches will be played on the weekend of March 5-6 with the venues yet to be confirmed. Although, Gloucester were informed by the RFU, prior to the draw, that the first and third teams drawn from the hat would have home advantage.
Ticket details, and date and kick off times will be confirmed shortly.
Speaking with the local media before the Gloucester team set off on the long trip north to Newcastle, Director of Rugby David Humphreys discussed his team selection and stated that Friday night's meeting will be a challenging one.
Speaking to the local media on Thursday, Matt Kvesic was looking forward to the trip to Newcastle Falcons, and the captain is looking to lead from the front with plenty for the Cherry and Whites to work on.
Gloucester saw a 12-0 lead overturned by Harlequins on Friday night as the visitors ran out 22-15 winners. After the game, Director of Rugby David Humphreys spoke about missed opportunities on the night.
Speaking to the local media on Thursday, James Hudson explained his excitement for tonight's game with Harlequins and that he is backing the Cherry and White squad to impress during the next set of Aviva Premiership games.
Despite a dream start at Kingston Park, scoring a try after just 26 seconds, Gloucester couldn't capitalise against a dogged Newcastle Falcons side. Too many penalties were conceded and too many errors made as the Falcons prevailed 20-10.
There are three changes to Gloucester Rugby's starting line-up for Friday evening's Aviva Premiership fixture away at Newcastle Falcons. Steve McColl will make his first Premiership start for the club.