Gloucester and Bath will meet in the semi-final of the Powergen Cup after the draw was made on Monday night.
The clash with Bath follows last weekend's West Country derby win over Bristol Shoguns in the quarter-final.
Commenting live on the radio after the draw Gloucester scrum-half Andy Gomarsall said: "I knew this was going to happen. We've just had one derby against Bristol so I thought another was on the cards.
"Derbies tend to bring out the best in us and the supporters. The atmosphere at Kingsholm against Bristol on Saturday was superb."
Gloucester clash with Bath in the Zurich Premiership at Kingsholm on Saturday 19 February in a 13,000 sell-out, and the Cup semi-final will take place on the weekend of the 5/6 March.
In the other semi-final Leeds, the only team in the draw not to have won the cup, and London Irish were paired in the other semi-final.
The Tykes' presence in the last four arrived courtesy of a 24-19 triumph over Northampton while Irish edged past Saracens 21-15 in a thrilling encounter at Vicarage Road yesterday.
Winger Justin Bishop, who was part of the Exiles side who claimed Powergen Cup glory in 2002, said: "This is a one-off game and hopefully we can get in the final again. That would be massive for our club.
"All the teams in the draw are quite similar at the moment - the big sides like Leicester and Wasps - so we weren't really bothered who we got."
Both matches will be played on the weekend of March 5-6 with the venues yet to be confirmed. Although, Gloucester were informed by the RFU, prior to the draw, that the first and third teams drawn from the hat would have home advantage.
Ticket details, and date and kick off times will be confirmed shortly.
Despite he and a number of his team mates playing out of position, Greig Laidlaw's four penalties were enough to help Gloucester to a 12-11 Aviva Premiership win at Sale on Friday evening. The skipper told GRTV after the game that everyone had played their part, especially the younger players.
Greig Laidlaw skippers the Cherry and Whites on Friday evening, when they head to Sale Sharks for the penultimate game of this season's Aviva Premiership. The Scottish half back spoke to GRTV about the challenge posed in Salford.
Previewing Friday night's Aviva Premiership fixture away at Sale Sharks, Head Coach Laurie Fisher acknowledged the fine home record that the Salford based outfit have built up this season, but is looking for Gloucester to round off their campaign on a high note.
Speaking to GRTV following the 16-9 victory over Exeter, Tom Savage felt that Gloucester needed that win after a tough few weeks and hopes that it acts as a springboard to a good end to the season for the Cherry and Whites.
A week on from '...letting down ourselves, the jersey and the supporters...', skipper Greig Laidlaw was full of praise for his team mates following the 16-9 Aviva Premiership win over Exeter Chiefs at Kingsholm on Friday evening.
Four penalties from the boot of skipper Greig Laidlaw were just enough to see Gloucester dog out a tense 12-11 win at Sale on Friday evening and thwart the home team's attempts to finish the season unbeaten at home.
Thirteen years on from Gloucester Rugby's famous Powergen Cup victory over Northampton at Twickenham, many of the players and management from that side are gathering once more to recreate that famous day.