Gloucester and Bath will meet in the semi-final of the Powergen Cup after the draw was made on Monday night.
The clash with Bath follows last weekend's West Country derby win over Bristol Shoguns in the quarter-final.
Commenting live on the radio after the draw Gloucester scrum-half Andy Gomarsall said: "I knew this was going to happen. We've just had one derby against Bristol so I thought another was on the cards.
"Derbies tend to bring out the best in us and the supporters. The atmosphere at Kingsholm against Bristol on Saturday was superb."
Gloucester clash with Bath in the Zurich Premiership at Kingsholm on Saturday 19 February in a 13,000 sell-out, and the Cup semi-final will take place on the weekend of the 5/6 March.
In the other semi-final Leeds, the only team in the draw not to have won the cup, and London Irish were paired in the other semi-final.
The Tykes' presence in the last four arrived courtesy of a 24-19 triumph over Northampton while Irish edged past Saracens 21-15 in a thrilling encounter at Vicarage Road yesterday.
Winger Justin Bishop, who was part of the Exiles side who claimed Powergen Cup glory in 2002, said: "This is a one-off game and hopefully we can get in the final again. That would be massive for our club.
"All the teams in the draw are quite similar at the moment - the big sides like Leicester and Wasps - so we weren't really bothered who we got."
Both matches will be played on the weekend of March 5-6 with the venues yet to be confirmed. Although, Gloucester were informed by the RFU, prior to the draw, that the first and third teams drawn from the hat would have home advantage.
Ticket details, and date and kick off times will be confirmed shortly.
Lloyd Evans will assume a senior role in the Gloucester Rugby squad as they head to Franklin's Gardens for the Singha Premiership Rugby 7s on Friday evening. The exciting Academy fly half spoke to GRTV this week ahead of the season curtain-raiser.
Summer signing Matt Scott met with the local media for the first time this week and said that he's settling in well at Gloucester, that hopes are high for the forthcoming season and that skipper Greig Laidlaw had done a good sales pitch when it came to him signing!
Olly Morgan is heading up Gloucester's Singha Premiership 7s once again this summer and, with the start of the tournament just around the corner, he's looking for some of the club's younger players to make the most of the playing time and the exposure it offers.
The Cherry & Whites are now in their second full week of preseason ahead of the new season, and GRTV caught up with the new Head of Strength & Conditioning Dan Tobin to chat about what is in store, and his first impressions of the club.
Speaking exclusively to GRTV before Wales set off for Twickenham on Friday afternoon, Ross Moriarty chatted about the prospect of taking on England on Sunday as well as the challenge of the subsequent tour to New Zealand.
Brighton is the venue of choice for England this week as the squad have reported for a training camp to prepare for the upcoming international against Wales. GRTV caught up with Gloucester's Matt Kvesic after the first day's training.
Speaking to GRTV after the game, Gloucester Rugby number eight Ben Morgan reflected on the loss to Northampton Saints, and spoke about what he and the team need to do for the new season when the team return after the summer break.
Cameron Orr was another new face in the preseason friendly at Leinster on Saturday and, following the game, spoke of how he's settled in at Gloucester and how he's looking to learn and contribute to the overall team effort.
Gloucester kicked off their 2016-17 preseason campaign with a narrow 26-24 defeat away at Leinster, a performance which Director of Rugby David Humphreys insisted gives the squad something to build on.