Gloucester and Bath will meet in the semi-final of the Powergen Cup after the draw was made on Monday night.
The clash with Bath follows last weekend's West Country derby win over Bristol Shoguns in the quarter-final.
Commenting live on the radio after the draw Gloucester scrum-half Andy Gomarsall said: "I knew this was going to happen. We've just had one derby against Bristol so I thought another was on the cards.
"Derbies tend to bring out the best in us and the supporters. The atmosphere at Kingsholm against Bristol on Saturday was superb."
Gloucester clash with Bath in the Zurich Premiership at Kingsholm on Saturday 19 February in a 13,000 sell-out, and the Cup semi-final will take place on the weekend of the 5/6 March.
In the other semi-final Leeds, the only team in the draw not to have won the cup, and London Irish were paired in the other semi-final.
The Tykes' presence in the last four arrived courtesy of a 24-19 triumph over Northampton while Irish edged past Saracens 21-15 in a thrilling encounter at Vicarage Road yesterday.
Winger Justin Bishop, who was part of the Exiles side who claimed Powergen Cup glory in 2002, said: "This is a one-off game and hopefully we can get in the final again. That would be massive for our club.
"All the teams in the draw are quite similar at the moment - the big sides like Leicester and Wasps - so we weren't really bothered who we got."
Both matches will be played on the weekend of March 5-6 with the venues yet to be confirmed. Although, Gloucester were informed by the RFU, prior to the draw, that the first and third teams drawn from the hat would have home advantage.
Ticket details, and date and kick off times will be confirmed shortly.
Gloucester-Hartpury Women take on West Park Leeds Ladies in the final of the Intermediate Cup at Royal Wootton Bassett RFC on Saturday. Rebecca de Filippo and Gemma Sharples took time out from training to preview the game.
Speaking to the local media this week, front row forward John Afoa spoke of the squad's targets for this weekend's European Rugby Challenge Cup Quarter Final with Cardiff Blues at Kingsholm. Despite taking on new opponents, the squad know the focus is on themselves to get the job done as knockout rugby kicks in.
Gloucester-Hartpury Women take on West Park Leeds Ladies in the final of the Intermediate Cup Final on Saturday. Head Coach Susie Appleby took time out to preview the game and chat about the future for the team.
Number eight Ben Morgan admitted that the 32-14 Aviva Premiership win over Bristol on Friday evening hadn't been pretty at times, but that the win was based on everyone in the squad remaining united and working for each other.
Previewing Friday evening's local derby at Bristol, Interim Head Coach Jonny Bell told the local media that Gloucester's preparations have gone well and that the whole squad is united in trying to finish the season on a high.
Speaking to GRTV this week, Acting Head Coach Jonny Bell spoke of his pleasure in the efforts and intensity in training from the squad after a tricky few weeks. The team were preparing for big games with Bristol and Cardiff in coming weeks.
Willi Heinz was understandably and visibly disappointed when he spoke to GRTV after the 27-30 home defeat to Harlequins, apologising to the home support as the game slipped away after seemingly having the game under control.
In the aftermath of the Aviva Premiership victory over Bristol, Interim Head Coach Jonny Bell was able to look forward to this weekend's European Rugby Challenge Cup quarter-final against Cardiff Blues (Kingsholm, kick off 8:05pm).