Gloucester and Bath will meet in the semi-final of the Powergen Cup after the draw was made on Monday night.
The clash with Bath follows last weekend's West Country derby win over Bristol Shoguns in the quarter-final.
Commenting live on the radio after the draw Gloucester scrum-half Andy Gomarsall said: "I knew this was going to happen. We've just had one derby against Bristol so I thought another was on the cards.
"Derbies tend to bring out the best in us and the supporters. The atmosphere at Kingsholm against Bristol on Saturday was superb."
Gloucester clash with Bath in the Zurich Premiership at Kingsholm on Saturday 19 February in a 13,000 sell-out, and the Cup semi-final will take place on the weekend of the 5/6 March.
In the other semi-final Leeds, the only team in the draw not to have won the cup, and London Irish were paired in the other semi-final.
The Tykes' presence in the last four arrived courtesy of a 24-19 triumph over Northampton while Irish edged past Saracens 21-15 in a thrilling encounter at Vicarage Road yesterday.
Winger Justin Bishop, who was part of the Exiles side who claimed Powergen Cup glory in 2002, said: "This is a one-off game and hopefully we can get in the final again. That would be massive for our club.
"All the teams in the draw are quite similar at the moment - the big sides like Leicester and Wasps - so we weren't really bothered who we got."
Both matches will be played on the weekend of March 5-6 with the venues yet to be confirmed. Although, Gloucester were informed by the RFU, prior to the draw, that the first and third teams drawn from the hat would have home advantage.
Ticket details, and date and kick off times will be confirmed shortly.
Gary Jones of Glevum Windows and Conservatories recently visited Kingsholm Stadium and the Cherry and White supporter spoke exclusively to Gloucester Rugby TV after marking two decades as a box holder in the Stowford Press Stand at Kingsholm. A great feat!
Speaking to the local media this week during preseason, new hooker Richard Hibbard said he was really enjoying putting in the hard work and being back to full fitness ahead of the 2014-15 season with the Cherry and Whites.
Nick Walshe was unveiled as Gloucester's new Backs and Attack Coach this week and spoke to Simon Ward for the RFU.com podcast about his excitement at joining Gloucester and working with a new group of players.
Speaking after the Third Test defeat to New Zealand, Ben Morgan told RFU.com that the loss was a disappointing way to sign off the summer tour but that the England team know what they have to do to be successful.
At the end of a tour when there have been so many positives for England, fly half Freddie Burns, who will start the third Test against New Zealand, was clear that the whole squad are focussed on signing off with a memorable win when he spoke to RFU.com
Speaking to Steve Jones of RFU.com straight after the 27-28 defeat to New Zealand on Saturday, Billy Twelvetrees spoke of the frustration of a missed opportunity, but stressed that England would lick their wounds and bounce back.
Freddie Burns contributed 12 points in England's 20-15 defeat against New Zealand on Saturday but, speaking after the game to RFU.com, preferred to talk about the many positives in the performance from Stuart Lancaster's side.
Gloucester number eight Ben Morgan put in a great individual performance as England were just pipped by New Zealand by 20 points to 15 in Auckland on Saturday. He told Steve Jones of RFU.com that the team will be in good spirits going into the 2nd Test in Dunedin.
It's been marked in the diary since the spring, but rugby will be returning to Kingsholm on July 31st. The holders host the Premiership Rugby Sevens for the third consecutive year, and Dan Robson can't wait to get on the field with the new look squad.
With almost a month of pre-season training with Gloucester Rugby already under his belt, James Voss is ready to hit the ground running as he links up with his Hartpury coaches and team-mates this week.
Premiership Rugby have today announced the fixtures for the second World Club Sevens tournament at Twickenham over the weekend of August 16-17 and Gloucester Rugby will kick off their Pool B campaign against Vodacom Blue Bulls.