Gloucester and Bath will meet in the semi-final of the Powergen Cup after the draw was made on Monday night.
The clash with Bath follows last weekend's West Country derby win over Bristol Shoguns in the quarter-final.
Commenting live on the radio after the draw Gloucester scrum-half Andy Gomarsall said: "I knew this was going to happen. We've just had one derby against Bristol so I thought another was on the cards.
"Derbies tend to bring out the best in us and the supporters. The atmosphere at Kingsholm against Bristol on Saturday was superb."
Gloucester clash with Bath in the Zurich Premiership at Kingsholm on Saturday 19 February in a 13,000 sell-out, and the Cup semi-final will take place on the weekend of the 5/6 March.
In the other semi-final Leeds, the only team in the draw not to have won the cup, and London Irish were paired in the other semi-final.
The Tykes' presence in the last four arrived courtesy of a 24-19 triumph over Northampton while Irish edged past Saracens 21-15 in a thrilling encounter at Vicarage Road yesterday.
Winger Justin Bishop, who was part of the Exiles side who claimed Powergen Cup glory in 2002, said: "This is a one-off game and hopefully we can get in the final again. That would be massive for our club.
"All the teams in the draw are quite similar at the moment - the big sides like Leicester and Wasps - so we weren't really bothered who we got."
Both matches will be played on the weekend of March 5-6 with the venues yet to be confirmed. Although, Gloucester were informed by the RFU, prior to the draw, that the first and third teams drawn from the hat would have home advantage.
Ticket details, and date and kick off times will be confirmed shortly.
Billy Twelvetrees spoke to Russ Hargreaves of Talking Rugby at England training ahead of Sunday's huge RBS Six Nations clash with Wales at Twickenham. The centre is relishing the challenge of fronting up against a smart and talented Welsh back line.
Tim Taylor spoke to Gloucester Rugby TV after the 31 year old fly half announced that he was retiring from professional rugby. The Derby-born pivot, is excited for what the future holds for his new business ventures despite his career being cut short by a knee injury.
Dan Robson spoke to Gloucester Rugby TV at Franklin's Gardens following the 39-13 loss to Saints. The scrum-half enjoyed his battle with Lee Dickson but was gutted at the result as the Cherry and Whites did not do themselves justice, falling off in the closing stages to the league leaders.
Speaking after the game at Franklin's Gardens, Director of Rugby Nigel Davies reflected on a disappointing final quarter which saw Northampton Saints ease their way to a comfortable 39-13 win on Saturday afternoon.
Gloucester Rugby TV caught up with Gareth Evans this week ahead of the trip to Northampton Saints in the Aviva Premiership. The back rower explained his pride in his performances this season but knows there is still a lot for him to learn.
Gloucester Rugby travel to take on the table topping Northampton Saints in the Aviva Premiership on Saturday. Director of Rugby Nigel Davies is well aware of the qualities of Jim Mallinder's side, but Gloucester are the only team to defeat the Saints in the league this season.
Gloucester number eight Ben Morgan is hoping to start against Wales a week on Sunday, a team containing a number of former club team mates. He told the RFU.com website that he's looking forward to the prospect.
Second row James Hudson spoke to the club website following the 25-20 victory over Harlequins about how missed chances made things nervy late on, on what was a hugely emotional day for the Gloucester team.
Dan Murphy spoke to Gloucester Rugby TV after the win over Harlequins. The front rower touched down for the crucial second half score and expressed his delight at the performance and insisted the importance to push on from it.
There will be a trio of Gloucester Rugby players in the England Rugby starting line up for Sunday's huge clash with Wales at Twickenham. Number eight Ben Morgan is named to start with Billy Twelvetrees and Jonny May continuing in the back line.
England Under 20s have named their side to take on Wales for Friday's Under 20 Six Nations clash at Kingston Park, and Gloucester Rugby duo Billy Burns and Ross Moriarty keep their places in the starting line-up, with Jonny Hill named on the bench.