Gloucester and Bath will meet in the semi-final of the Powergen Cup after the draw was made on Monday night.
The clash with Bath follows last weekend's West Country derby win over Bristol Shoguns in the quarter-final.
Commenting live on the radio after the draw Gloucester scrum-half Andy Gomarsall said: "I knew this was going to happen. We've just had one derby against Bristol so I thought another was on the cards.
"Derbies tend to bring out the best in us and the supporters. The atmosphere at Kingsholm against Bristol on Saturday was superb."
Gloucester clash with Bath in the Zurich Premiership at Kingsholm on Saturday 19 February in a 13,000 sell-out, and the Cup semi-final will take place on the weekend of the 5/6 March.
In the other semi-final Leeds, the only team in the draw not to have won the cup, and London Irish were paired in the other semi-final.
The Tykes' presence in the last four arrived courtesy of a 24-19 triumph over Northampton while Irish edged past Saracens 21-15 in a thrilling encounter at Vicarage Road yesterday.
Winger Justin Bishop, who was part of the Exiles side who claimed Powergen Cup glory in 2002, said: "This is a one-off game and hopefully we can get in the final again. That would be massive for our club.
"All the teams in the draw are quite similar at the moment - the big sides like Leicester and Wasps - so we weren't really bothered who we got."
Both matches will be played on the weekend of March 5-6 with the venues yet to be confirmed. Although, Gloucester were informed by the RFU, prior to the draw, that the first and third teams drawn from the hat would have home advantage.
Ticket details, and date and kick off times will be confirmed shortly.
Having skippered Scotland to a hard fought 25-13 defeat against England at Twickenham on Saturday, Greig Laidlaw spoke of the frustrations of his side doing so many good things and making real progress as a team but still finishing on the wrong end of the scoreline.
GRTV caught up with Neil McCarthy as he was unveiled as the new Head of Academy. The ex-Gloucester front rower is looking forward to getting to work with the vast arrays of talent in the Academy and across Gloucestershire.
Although understandably disappointed to see Northampton snatch a 33-33 draw with a last gasp try on Saturday, Gareth Evans told GRTV after the game that the result shows how far Gloucester have come as a team this season.
Gloucester scrum half Greig Laidlaw spoke to GRTV after the 33-33 draw with Northampton Saints on Saturday, and the Scotsman described how the hosts played some great rugby, but will rue not holding the lead which keeps them in touch with the top seven.
Although Gloucester could take many positives from the 33-33 draw with Northampton Saints on Saturday, centre Bill Meakes admitted it was a strange feeling at the final whistle with Gloucester having probably done enough to win the game.
Speaking after the 33-33 draw with Northampton Saints on Saturday, David Humphreys admitted that he was happy with many aspects of the team's performance but regretted a missed opportunity to defeat the Premiership champions.
Speaking to the local media this week, Richard Hibbard is relishing his return to the Cherry and White jersey as the hosts take on Aviva Premiership leaders Northampton Saints on Saturday afternoon (ko 3:15pm)
Ahead of the visit of Northampton Saints to Kingsholm in the Aviva Premiership on Saturday (kick off 3:15pm), Head Coach Laurie Fisher admitted that it's good to have more selection options as the RBS Six Nations takes a break.
Fresh from his first training session with the Cherry and Whites, new arrival Brendan Macken spoke exclusively to GRTV ahead of his time at Kingsholm. The Leinsterman is keen to experience Kingsholm and the rugby heritage at Gloucester.
Gloucester Rugby would like to inform supporters about an exciting opportunity for you to raise money for your chosen charity or chosen cause. 2015 is an exciting year for Gloucester, with Kingsholm Stadium hosting four fixtures for Rugby World Cup 2015.
On loan centre Brendan Macken will make his first start for Gloucester Rugby as the Cherry and Whites resume their Aviva Premiership campaign on Sunday when they take on the Sale Sharks at the AJ Bell Stadium.
Gloucester Rugby are used to repaying their loyal supporters with blistering displays on the pitch, but prop Dan Murphy insists there is now another way to give back with the launch of the Aviva Community Fund.