Gloucester and Bath will meet in the semi-final of the Powergen Cup after the draw was made on Monday night.
The clash with Bath follows last weekend's West Country derby win over Bristol Shoguns in the quarter-final.
Commenting live on the radio after the draw Gloucester scrum-half Andy Gomarsall said: "I knew this was going to happen. We've just had one derby against Bristol so I thought another was on the cards.
"Derbies tend to bring out the best in us and the supporters. The atmosphere at Kingsholm against Bristol on Saturday was superb."
Gloucester clash with Bath in the Zurich Premiership at Kingsholm on Saturday 19 February in a 13,000 sell-out, and the Cup semi-final will take place on the weekend of the 5/6 March.
In the other semi-final Leeds, the only team in the draw not to have won the cup, and London Irish were paired in the other semi-final.
The Tykes' presence in the last four arrived courtesy of a 24-19 triumph over Northampton while Irish edged past Saracens 21-15 in a thrilling encounter at Vicarage Road yesterday.
Winger Justin Bishop, who was part of the Exiles side who claimed Powergen Cup glory in 2002, said: "This is a one-off game and hopefully we can get in the final again. That would be massive for our club.
"All the teams in the draw are quite similar at the moment - the big sides like Leicester and Wasps - so we weren't really bothered who we got."
Both matches will be played on the weekend of March 5-6 with the venues yet to be confirmed. Although, Gloucester were informed by the RFU, prior to the draw, that the first and third teams drawn from the hat would have home advantage.
Ticket details, and date and kick off times will be confirmed shortly.
Speaking to the media after the 45-8 preseason friendly victory over Munster, Director of Rugby David Humphreys wasn't getting too carried away, admitting that his team are making progress but that there's still a way to go.
Billy Twelvetrees spoke to Gloucester Rugby TV on Friday ahead of the pre season friendly with Munster Rugby. The club captain is excited to be back in the action at Kingsholm and experience the atmosphere again.
Gloucester Rugby Director of Rugby David Humphreys spoke to the local media after the 62-19 victory over Yorkshire Carnegie, and showed his delight at the first run out and stated there was still lots to work on ahead of the new season
Mark Atkinson made his first appearance in Cherry and White in the pre season victory over Yorkshire Carnegie on Saturday. Speaking to the local media, he was impressed with the first experience of Kingsholm.
James Hook picked himself up a pair of tries on his first appearance in Cherry and Whites during the 62-19 win over Yorkshire Carnegie. Speaking to the media after, the Welshman was thrilled with the first run out as well as the result.
England Rugby 2015, the Organising Committee for Rugby World Cup 2015 (RWC 2015), has announced the list of 41 Team Bases which will host the 20 participating teams during the Tournament, taking place in England and in Cardiff between September 18 and October 31 2015.
With the Gloucestershire weather taking a turn for the worst on Bank Holiday Monday, the club's Open Day will still be going ahead despite the rain, and Gloucester Rugby are looking forward to welcoming you to Kingsholm.