Gloucester and Bath will meet in the semi-final of the Powergen Cup after the draw was made on Monday night.
The clash with Bath follows last weekend's West Country derby win over Bristol Shoguns in the quarter-final.
Commenting live on the radio after the draw Gloucester scrum-half Andy Gomarsall said: "I knew this was going to happen. We've just had one derby against Bristol so I thought another was on the cards.
"Derbies tend to bring out the best in us and the supporters. The atmosphere at Kingsholm against Bristol on Saturday was superb."
Gloucester clash with Bath in the Zurich Premiership at Kingsholm on Saturday 19 February in a 13,000 sell-out, and the Cup semi-final will take place on the weekend of the 5/6 March.
In the other semi-final Leeds, the only team in the draw not to have won the cup, and London Irish were paired in the other semi-final.
The Tykes' presence in the last four arrived courtesy of a 24-19 triumph over Northampton while Irish edged past Saracens 21-15 in a thrilling encounter at Vicarage Road yesterday.
Winger Justin Bishop, who was part of the Exiles side who claimed Powergen Cup glory in 2002, said: "This is a one-off game and hopefully we can get in the final again. That would be massive for our club.
"All the teams in the draw are quite similar at the moment - the big sides like Leicester and Wasps - so we weren't really bothered who we got."
Both matches will be played on the weekend of March 5-6 with the venues yet to be confirmed. Although, Gloucester were informed by the RFU, prior to the draw, that the first and third teams drawn from the hat would have home advantage.
Ticket details, and date and kick off times will be confirmed shortly.
Billy Twelvetrees spoke to Gloucester Rugby TV after contributing fifteen points for the team at Twickenham. Despite the defeat, Twelvetrees praised the effort shown by his team, but rued silly errors that gifted London Wasps easy points.
Director of Rugby Nigel Davies was quickly able to identify a couple of key areas where Gloucester weren't quite good enough on the day, when he spoke to the media following defeat in The Stinger at Twickenham.
Huia Edmonds spoke to Gloucester Rugby TV this week ahead of Gloucester's clash with London Wasps in #TheStinger. The Australian hooker is yet to play at Twickenham and is excited at the prospect and putting things right on the pitch.
The bitter disappointment of losing against London Wasps in the Amlin Challenge Cup less than a fortnight ago, will give Gloucester ample motivation against the same opponent at Twickenham on Saturday says Director of Rugby Nigel Davies.
Billy Twelvetrees spoke to Gloucester Rugby TV following the 17-18 loss to Bath Rugby in Round Nineteen of Aviva Premiership Rugby. The Gloucester skipper said he was proud of the desire shown by his team at the end of a tough afternoon.
Nigel Davies spoke to Gloucester Rugby TV following the gutting 17-18 reverse to Bath on Saturday afternoon. The Director of Rugby expressed his pride at the efforts from his players and thanked the supporters for their incredible backing.
Nick Wood spoke to Gloucester Rugby TV this week ahead of Saturday's West Country derby clash with Bath Rugby. The front rower called on the squad to make a strong start and get the Kingsholm crowd behind them
Director of Rugby Nigel Davies admits that it's been a tough week at Gloucester Rugby following defeat at London Wasps last weekend, but that he's looking for his team to rise to the occasion for the huge game at home to Bath Rugby on Saturday.
Gloucester Rugby's trip to Twickenham ended in a 38-30 defeat to London Wasps in the sunshine. The hosts ran in 5 tries to Gloucester's three on a tough afternoon that despite plenty of promise saw Gloucester let down by errors.