Gloucester and Bath will meet in the semi-final of the Powergen Cup after the draw was made on Monday night.
The clash with Bath follows last weekend's West Country derby win over Bristol Shoguns in the quarter-final.
Commenting live on the radio after the draw Gloucester scrum-half Andy Gomarsall said: "I knew this was going to happen. We've just had one derby against Bristol so I thought another was on the cards.
"Derbies tend to bring out the best in us and the supporters. The atmosphere at Kingsholm against Bristol on Saturday was superb."
Gloucester clash with Bath in the Zurich Premiership at Kingsholm on Saturday 19 February in a 13,000 sell-out, and the Cup semi-final will take place on the weekend of the 5/6 March.
In the other semi-final Leeds, the only team in the draw not to have won the cup, and London Irish were paired in the other semi-final.
The Tykes' presence in the last four arrived courtesy of a 24-19 triumph over Northampton while Irish edged past Saracens 21-15 in a thrilling encounter at Vicarage Road yesterday.
Winger Justin Bishop, who was part of the Exiles side who claimed Powergen Cup glory in 2002, said: "This is a one-off game and hopefully we can get in the final again. That would be massive for our club.
"All the teams in the draw are quite similar at the moment - the big sides like Leicester and Wasps - so we weren't really bothered who we got."
Both matches will be played on the weekend of March 5-6 with the venues yet to be confirmed. Although, Gloucester were informed by the RFU, prior to the draw, that the first and third teams drawn from the hat would have home advantage.
Ticket details, and date and kick off times will be confirmed shortly.
Billy Burns and Man of the Match Richard Hibbard spoke to GRTV after the 41-0 win in Treviso, on a day when preparations weren't ideal. They felt the team's attitude was key and also praised the travelling support.
Matt Scott was again in impressive try scoring form for the Cherry and Whites on Saturday, and the Scotsman laid praise on his fellow backs for their attacking skills in a big 55-19 win over Worcester Warriors
Jonny May spilled blood for the cause on Saturday, before going on to score the first try in the 55-19 Aviva Premiership victory over Worcester. He told GRTV that the win was a reward for the hard work the squad have been putting in.
Gloucester Rugby face their second tough Aviva Premiership fixture in six days when they take on Worcester Warriors at Kingsholm on Saturday (kick off 3pm). Head Coach Laurie Fisher is expecting a massive challenge from one of Gloucester's local rivals.
It was a frustrated and bitterly disappointed Willi Heinz who spoke to GRTV following the 12-13 reverse to Northampton Saints on New Year's Day. The skipper thanked the fans for their incredible support on a day when Gloucester couldn't quite get the job done.