Gloucester and Bath will meet in the semi-final of the Powergen Cup after the draw was made on Monday night.
The clash with Bath follows last weekend's West Country derby win over Bristol Shoguns in the quarter-final.
Commenting live on the radio after the draw Gloucester scrum-half Andy Gomarsall said: "I knew this was going to happen. We've just had one derby against Bristol so I thought another was on the cards.
"Derbies tend to bring out the best in us and the supporters. The atmosphere at Kingsholm against Bristol on Saturday was superb."
Gloucester clash with Bath in the Zurich Premiership at Kingsholm on Saturday 19 February in a 13,000 sell-out, and the Cup semi-final will take place on the weekend of the 5/6 March.
In the other semi-final Leeds, the only team in the draw not to have won the cup, and London Irish were paired in the other semi-final.
The Tykes' presence in the last four arrived courtesy of a 24-19 triumph over Northampton while Irish edged past Saracens 21-15 in a thrilling encounter at Vicarage Road yesterday.
Winger Justin Bishop, who was part of the Exiles side who claimed Powergen Cup glory in 2002, said: "This is a one-off game and hopefully we can get in the final again. That would be massive for our club.
"All the teams in the draw are quite similar at the moment - the big sides like Leicester and Wasps - so we weren't really bothered who we got."
Both matches will be played on the weekend of March 5-6 with the venues yet to be confirmed. Although, Gloucester were informed by the RFU, prior to the draw, that the first and third teams drawn from the hat would have home advantage.
Ticket details, and date and kick off times will be confirmed shortly.
Ben Morgan, Tom Johnson and Henry Thomas spoke to Russ Hargreaves this week as they were about to do some DIY work at Chobham RFC as part of Natwest Rugby Force. On the agenda for discussion were, of course, England's forthcoming games but also the trio's painting prowess.
With ADEY Professional Heating Solutions now unveiled as Gloucester Rugby's new main sponsor from July 2013, managing Director Kelvin Stevens spoke to the club website about the reasons for getting involved with the club.
Gloucester Rugby CEO Stephen Vaughan was delighted to introduce ADEY Professional Heating Solutions as the club's new main sponsor this week. He spoke to the club website about the exciting new partnership.
Although understandably disappointed following the 40-39 defeat at Exeter, Freddie Burns thought that both teams fully contributed to a great spectacle but that a couple of refereeing decisions were key to the outcome.
Gloucester's final game of the season ended in a narrow defeat in a pulsating encounter at the Exeter Chiefs. The Cherry and Whites went down 40-39, as Gareth Steenson's dramatic late penalty meant that the end of Director of Rugby Nigel Davies' first season in charge ended in defeat. Davies spoke to the official website in the Devon sunshine.
Dave Lewis was in a tough situation on Saturday afternoon - playing for Gloucester against the side he will join next season, Exeter Chiefs. At the final whistle though, Lewis was simply disappointed to have lost the game.
Tongan second row Lua Lokotui is looking forward to catching up with some old acquaintances at Sandy Park when Gloucester take on Exeter Chiefs on Saturday afternoon, as he and his team mates hope to finish the season on a high note.
During a break from training ahead of Saturday's Aviva Premiership fixture at Exeter, centre Henry Trinder hopes that Gloucester build on the momentum from the recent win over Saracens, and expressed frustration that he's hitting form as the season end approaches after missing time due to injury.
Gloucester Rugby may have already clinched their Heineken Cup spot for 2013-14, but Director of Rugby Nigel Davies stressed this week that it's important for Gloucester to finish their season on a positive note at Exeter on Saturday.