Gloucester and Bath will meet in the semi-final of the Powergen Cup after the draw was made on Monday night.
The clash with Bath follows last weekend's West Country derby win over Bristol Shoguns in the quarter-final.
Commenting live on the radio after the draw Gloucester scrum-half Andy Gomarsall said: "I knew this was going to happen. We've just had one derby against Bristol so I thought another was on the cards.
"Derbies tend to bring out the best in us and the supporters. The atmosphere at Kingsholm against Bristol on Saturday was superb."
Gloucester clash with Bath in the Zurich Premiership at Kingsholm on Saturday 19 February in a 13,000 sell-out, and the Cup semi-final will take place on the weekend of the 5/6 March.
In the other semi-final Leeds, the only team in the draw not to have won the cup, and London Irish were paired in the other semi-final.
The Tykes' presence in the last four arrived courtesy of a 24-19 triumph over Northampton while Irish edged past Saracens 21-15 in a thrilling encounter at Vicarage Road yesterday.
Winger Justin Bishop, who was part of the Exiles side who claimed Powergen Cup glory in 2002, said: "This is a one-off game and hopefully we can get in the final again. That would be massive for our club.
"All the teams in the draw are quite similar at the moment - the big sides like Leicester and Wasps - so we weren't really bothered who we got."
Both matches will be played on the weekend of March 5-6 with the venues yet to be confirmed. Although, Gloucester were informed by the RFU, prior to the draw, that the first and third teams drawn from the hat would have home advantage.
Ticket details, and date and kick off times will be confirmed shortly.
Speaking to GRTV this afternoon, Director of Rugby David Humphreys hailed the 'never say die' attitude of the team, after despite not being at their best, fighting back to take a 40-32 win after extra time over Connacht.
Jonny May continues on the wing on Sunday as Connacht come to town, and the England wing explains to the local media this week that he is delighted with his form and is looking forward to taking on Pat Lam's men.
Speaking to the local media this week, front rower John Afoa is familiar with the challenge of Connacht from his time in Belfast, and the World Cup winner is fully aware of the challenge they pose ahead of Sunday's meeting in the Champions Cup play off
Speaking ahead of Gloucester's European Rugby Champions play-off against Connacht on Sunday (Kingsholm, 3:30pm), Director of Rugby David Humphreys admitted that he's glad to have more players available than in recent weeks ahead of a tough fixture.
Nick Wood captained the Cherry and Whites on Saturday afternoon, and the experienced front rower expressed his pride in the young squad in defeat to Bath on the final day of the Aviva Premiership season.
Lewis Ludlow scored Gloucester's opening try in the 30-50 defeat at Bath on Saturday, part of an impressive performance by the young back row forward. Speaking after the game, he paid tribute to the young Gloucester side who went toe to toe with the Aviva Premiership semi-finalists.
Speaking to the local media after the game, Director of Rugby David Humphreys gave an honest assessment of the game, and expressed his pride in the way his young charges had performed against the title-chasing hosts.
Speaking to the local media this week, Gloucester Rugby scrum half Callum Braley is excited by the challenge of the West Country Derby, and the ex-Bristol man reveals the squad will be giving their all.
Speaking to the local media this week, David Humphreys admitted that team selection for Saturday's Aviva Premiership fixture at Bath has not been an easy one, with numerous players ruled out due to injury and suspension. However, he pledged that whoever is wearing the Cherry and White will be 100% committed to fighting until the very last minute.
It was a thriller at Kingsholm on Sunday afternoon, as Gloucester snatched victory from the jaws of defeat, leveling the scores in the final minute before triumphing in extra time over a spirited Connacht side.
Having defeated Connacht Rugby in dramatic fashion in the first play-off for the final spot in the 2015-16 European Rugby Champions Cup, Gloucester Rugby can confirm ticketing details for next Sunday's game against Bordeaux-Begles at Sixways.
Gloucester snatched victory from the jaws of defeat and move on to next weekend's European Champions Cup final play-off against Bordeaux-Begles after a dramatic extra time win over a valiant Connacht side.
With Connacht Rugby the visitors to Kingsholm on Sunday (kick off 3:30pm) for Sunday's European Rugby Champions Cup play-off, Gloucester ring the changes from the side that took the field against Bath last weekend.
Matt Kvesic was a double winner at the Gloucester Rugby End of Season Awards Dinner on Tuesday, coupled with being named in the England Rugby training squad and the 23-year old is delighted with how his campaign has gone.