Gloucester's Powergen Cup semi-final against Bath will take place at the Club's Kingsholm ground.
The semi-final draw was made on Monday evening and paired the West Country sides, with Leeds and London Irish playing each other in the other semi.
After Gloucester's name was drawn out of the hat first, they received 'home advantage' and have been in discussions this week with the RFU to confirm the venue of the massive cup clash.
Gloucester will announce all ticket details for the Kingsholm fixture once the date and time are confirmed following further discussions with the RFU and BBC, who will broadcast both semi-finals live. The games are set to be played on the weekend of the 5/6 March.
Commenting on http://www.gloucesterrugbyclub.com Gloucester managing director Ken Nottage said: "We're delighted to be playing the game at Kingsholm. Everyone knows how good an occasion a Gloucester Vs Bath game is, and the semi-final will be up there with the best of them with a place in the Twickenham final up for grabs. As soon as we know the date and time of the fixture we'll be able to confirm ticket arrangements."
The other semi-final between Leeds Tykes and London Irish will be played at Headingley.
Terry Burwell, Rugby Football Union Community Rugby and Operations Director said: "We are delighted to be able to announce the venues for the semi-finals for what we are sure will be two great games. However, until the broadcasting schedule has been finalised we are unable to announce the exact dates and times of the games.
"We apologise for the delay and will endeavour to confirm arrangements with the clubs as soon as possible."
Gloucester recorded a 32-20 win over La Rochelle on Thursday evening, and second row Jeremy Thrush was thrilled to pick up the victory on his first taste of a trip to France and the hostile atmosphere.
Gloucester were forced to battle for their 33-20 victory in La Rochelle on Thursday evening, something that centre Bill Meakes said that he and the squad were well prepared for as well as thanking the travelling support for the part they played.
Gloucester got off to a winning start in the European Rugby Challenge Cup last weekend, but Head Coach Laurie Fisher is expecting a much tougher challenge when the Cherry and Whites travel to La Rochelle for Thursday night's Round Two encounter.
Dan Thomas was a try scorer in the 14-62 defeat at the hands of the Barbarians on Tuesday evening, but told GRTV after the game that he'd really enjoyed taking on the Barbarians and locking horns with Ardie Savea at the breakdown.
After a long, hard battle to return to fitness, Henry Trinder made his long awaited comeback in the second half of the 23-10 win over Zebre on Saturday and admitted he was taken aback by the reception he got from the Kingsholm crowd.
21 year old number eight Lewis Ludlow formed part of a physical back row effort in the 23-10 European Rugby Challenge Cup over Zebre on Saturday. Speaking to GRTV after the game, he spoke of making the most of his chances and being happy to contribute to the Gloucester win.
Gloucester United kick off their Aviva Premiership A League campaign against Leicester Tigers at Welford Road on Monday evening (kick off 7pm) and have named a side which features lots of first team experience.