Gloucester's Powergen Cup semi-final against Bath will take place at the Club's Kingsholm ground.
The semi-final draw was made on Monday evening and paired the West Country sides, with Leeds and London Irish playing each other in the other semi.
After Gloucester's name was drawn out of the hat first, they received 'home advantage' and have been in discussions this week with the RFU to confirm the venue of the massive cup clash.
Gloucester will announce all ticket details for the Kingsholm fixture once the date and time are confirmed following further discussions with the RFU and BBC, who will broadcast both semi-finals live. The games are set to be played on the weekend of the 5/6 March.
Commenting on http://www.gloucesterrugbyclub.com Gloucester managing director Ken Nottage said: "We're delighted to be playing the game at Kingsholm. Everyone knows how good an occasion a Gloucester Vs Bath game is, and the semi-final will be up there with the best of them with a place in the Twickenham final up for grabs. As soon as we know the date and time of the fixture we'll be able to confirm ticket arrangements."
The other semi-final between Leeds Tykes and London Irish will be played at Headingley.
Terry Burwell, Rugby Football Union Community Rugby and Operations Director said: "We are delighted to be able to announce the venues for the semi-finals for what we are sure will be two great games. However, until the broadcasting schedule has been finalised we are unable to announce the exact dates and times of the games.
"We apologise for the delay and will endeavour to confirm arrangements with the clubs as soon as possible."
Following the 48-10 Aviva Premiership win over London Welsh on Saturday, Dan Murphy praised his team mates for battling right up to the very last minute in difficult conditions and maintaining the momentum that the team have built in 2015.
Henry Purdy scored two memorable tries against London Welsh at Kingsholm on Saturday, and attributed the first one to hard work on the training ground before describing how much he's enjoying playing his rugby alongside friends like Callum Braley.
Gloucester face London Welsh for the third time this season when they meet at Kingsholm in the Aviva Premiership on Saturday (kick off 3pm), but Director of Rugby David Humphreys insists his team are prepared for a tough game against the Exiles.
Billy Burns was a late call up to the Gloucester team that lost narrowly to the Leicester Tigers at Welford Road on Friday evening and, speaking to GRTV post match, was full of praise for his team-mates but admitted that defeat was tough to take.
Speaking after what he described as an 'arm wrestle' up front, skipper Tom Savage couldn't fault his side's effort and commitment on the night at Welford Road but insisted that his side will keep on battling, starting at home to London Welsh next weekend.
A number of the Gloucester Rugby squad this week benefitted from an initiative organised by Circle 2 Success (C2S) and the Rugby Players Association (RPA) to help players develop their business planning skills.