Gloucester's Powergen Cup semi-final against Bath will take place at the Club's Kingsholm ground.
The semi-final draw was made on Monday evening and paired the West Country sides, with Leeds and London Irish playing each other in the other semi.
After Gloucester's name was drawn out of the hat first, they received 'home advantage' and have been in discussions this week with the RFU to confirm the venue of the massive cup clash.
Gloucester will announce all ticket details for the Kingsholm fixture once the date and time are confirmed following further discussions with the RFU and BBC, who will broadcast both semi-finals live. The games are set to be played on the weekend of the 5/6 March.
Commenting on http://www.gloucesterrugbyclub.com Gloucester managing director Ken Nottage said: "We're delighted to be playing the game at Kingsholm. Everyone knows how good an occasion a Gloucester Vs Bath game is, and the semi-final will be up there with the best of them with a place in the Twickenham final up for grabs. As soon as we know the date and time of the fixture we'll be able to confirm ticket arrangements."
The other semi-final between Leeds Tykes and London Irish will be played at Headingley.
Terry Burwell, Rugby Football Union Community Rugby and Operations Director said: "We are delighted to be able to announce the venues for the semi-finals for what we are sure will be two great games. However, until the broadcasting schedule has been finalised we are unable to announce the exact dates and times of the games.
"We apologise for the delay and will endeavour to confirm arrangements with the clubs as soon as possible."
Tom Marshall scored his first Gloucester try in the 31-23 win over Saracens on Friday evening and was named Man of the Match. Talking to GRTV, he praised the forwards for setting the tone on the night.
Matt Scott was on media duty this week, and the Scottish international spoke about the benefit of having a full week of training with his club mates and the determination to get back to performing well against Saracens this Friday evening.
After the disappointment of last weekend's performance at Leicester, Director of Rugby David Humphreys told GRTV that he expects a big response from Gloucester in Friday's Aviva Premiership fixture at home to Saracens (kick off 7:45pm).
Gloucester travel to Tigers this Saturday in good spirits, and fly half Billy Burns is looking forward to testing himself in one of the toughest environments in the league as the Cherry and White hunt vital points as attention returns to the Aviva Premiership
After a good run of recent results, Gloucester will travel to take on Leicester in a good frame of mind according to Director of Rugby David Humphreys, but are well aware of the challenge that lies in wait.
Gloucester Rugby continues to strengthen its commitment to working across Gloucestershire and the surrounding area and is looking to recruit an enthusiastic, motivated and dedicated individual to the role of Wheelchair Rugby Coach
Wales have named their side to face Scotland at Murrayfield on Saturday. They make just one change as George North returns in place of Alex Cuthbert and Gloucester's Ross Moriarty starts again at number eight.
Sunday saw Gloucester Rugby host the Land Rover Cup at Dean Close School in Cheltenham, and it was Tewkesbury and Old Pats who will represent the club at Twickenham in the U11s and U12s categories respectively.