Gloucester's Powergen Cup semi-final against Bath will take place at the Club's Kingsholm ground.
The semi-final draw was made on Monday evening and paired the West Country sides, with Leeds and London Irish playing each other in the other semi.
After Gloucester's name was drawn out of the hat first, they received 'home advantage' and have been in discussions this week with the RFU to confirm the venue of the massive cup clash.
Gloucester will announce all ticket details for the Kingsholm fixture once the date and time are confirmed following further discussions with the RFU and BBC, who will broadcast both semi-finals live. The games are set to be played on the weekend of the 5/6 March.
Commenting on http://www.gloucesterrugbyclub.com Gloucester managing director Ken Nottage said: "We're delighted to be playing the game at Kingsholm. Everyone knows how good an occasion a Gloucester Vs Bath game is, and the semi-final will be up there with the best of them with a place in the Twickenham final up for grabs. As soon as we know the date and time of the fixture we'll be able to confirm ticket arrangements."
The other semi-final between Leeds Tykes and London Irish will be played at Headingley.
Terry Burwell, Rugby Football Union Community Rugby and Operations Director said: "We are delighted to be able to announce the venues for the semi-finals for what we are sure will be two great games. However, until the broadcasting schedule has been finalised we are unable to announce the exact dates and times of the games.
"We apologise for the delay and will endeavour to confirm arrangements with the clubs as soon as possible."
Dan Murphy was part of a Gloucester pack which took a grip as the Sale game progressed and, ultimately, were rewarded with a late penalty try. The loose head prop says the team's improved scrummaging is down to a lot of hard work over the summer.
Gloucester clinched their first Aviva Premiership win of the season on Saturday, 34-27 over Sale Sharks, and scrum half Greig Laidlaw felt that the Kingsholm crowd played a massive part as they spurred the team on.
David Humphreys spoke to Gloucester Rugby TV after the 34-27 win over Sale Sharks at Kingsholm. The Director of Rugby praised the Kingsholm crowd for spurring the team on to a super second half comeback.
Speaking to Gloucester Rugby TV this week, Sione Kalamafoni is expecting a tough, physical battle against Sale Sharks as the Cherry and Whites return to Kingsholm for Round Two of the Aviva Premiership
Previewing this weekend's Aviva Premiership fixture at home to Sale Sharks with the local media, Director of Media David Humphreys admitted he's looking forward to the team's first competitive outing in front of their own supporters.
Harlequins' intriguing trip to Kingsholm kicks off another bumper set of live Aviva Premiership Rugby matches on BT Sport. The 2012 Aviva Premiership Rugby Champions will now travel to face David Humphreys' Gloucester Rugby side on Friday 14 November for a 7.45pm kick-off.
Gloucester United got off to a flying start with an early try in this evening's Aviva Premiership A League fixture at Worcester Cavaliers, but it was downhill from there as the home side dominated in a 34-17 win.
Triumphant in their challenge, the Gloucester Rugby Former players have successfully completed the 969 mile journey from Land's End to John O'Groats despite the aches and pains they endured on the way.