Gloucester's Powergen Cup semi-final against Bath will take place at the Club's Kingsholm ground.
The semi-final draw was made on Monday evening and paired the West Country sides, with Leeds and London Irish playing each other in the other semi.
After Gloucester's name was drawn out of the hat first, they received 'home advantage' and have been in discussions this week with the RFU to confirm the venue of the massive cup clash.
Gloucester will announce all ticket details for the Kingsholm fixture once the date and time are confirmed following further discussions with the RFU and BBC, who will broadcast both semi-finals live. The games are set to be played on the weekend of the 5/6 March.
Commenting on http://www.gloucesterrugbyclub.com Gloucester managing director Ken Nottage said: "We're delighted to be playing the game at Kingsholm. Everyone knows how good an occasion a Gloucester Vs Bath game is, and the semi-final will be up there with the best of them with a place in the Twickenham final up for grabs. As soon as we know the date and time of the fixture we'll be able to confirm ticket arrangements."
The other semi-final between Leeds Tykes and London Irish will be played at Headingley.
Terry Burwell, Rugby Football Union Community Rugby and Operations Director said: "We are delighted to be able to announce the venues for the semi-finals for what we are sure will be two great games. However, until the broadcasting schedule has been finalised we are unable to announce the exact dates and times of the games.
"We apologise for the delay and will endeavour to confirm arrangements with the clubs as soon as possible."
Previewing Friday evening's local derby at Bristol, Interim Head Coach Jonny Bell told the local media that Gloucester's preparations have gone well and that the whole squad is united in trying to finish the season on a high.
Speaking to GRTV this week, Acting Head Coach Jonny Bell spoke of his pleasure in the efforts and intensity in training from the squad after a tricky few weeks. The team were preparing for big games with Bristol and Cardiff in coming weeks.
Willi Heinz was understandably and visibly disappointed when he spoke to GRTV after the 27-30 home defeat to Harlequins, apologising to the home support as the game slipped away after seemingly having the game under control.
Speaking to the local media this week, Scottish centre Matt Scott spoke of the squad's determination to put in a big home performance against Harlequins, with vital points up for grabs in the hunt for a Top Six spot this Saturday (KO 3pm)
Looking ahead to Saturday's Aviva Premiership fixture at home to Harlequins (kick off 3pm), Head Coach Laurie Fisher told the local media that Gloucester will need to be on top of their game in a match which has big implications in terms of top six ambitions.
A big few days for the Gloucester-Hartpury Women side. First came the news that the team will compete in Women's Super Rugby next season, and on Saturday the team will play Worcester Ladies IIs at Kingsholm. Centre Hannah Jones and wing Michelle Ruddock spoke to GRTV this week to reflect on events.
Missing so many starters, and up against a team fighting for their Premiership existence, Gloucester did what had to be done at Ashton Gate on Friday evening battling their way to a 32-14 Aviva Premiership win over Bristol Rugby.