Sir Clive Woodward has confirmed his squad for the summer's British Lions tour, with Ireland's Brian O'Driscoll leading the side.
Gloucester's Phil Vickery, Newcastle's Jonny Wilkinson, and new Kingsholm recruit Mike Tindall all missed out.
Commenting exclusively on http://www.gloucesterrugbyclub.com/ after Monday morning's training session at Kingsholm, Vickery said: "It's a privilege to have gone on a Lions tour back in 2001. Realistically, you can't expect to be picked if you haven't been playing. If I'm honest then it's the right call."
28 year-old Vickery has had an injury-hit season with his recovery from back surgery followed by a blow-out fracture around his eye, and now a broken arm.
"The Lions will need players who are 100 per cent fit, and there are a few in my position. I have to get back playing for Gloucester, and that is first and foremost on my mind."
Despite not being listed in the 44-man squad, Woodward still offered Vickery, Wilkinson and Tindall a slight chance to still travel to New Zealand.
"If they confirm genuine form and fitness in the coming weeks, they could still go," said Woodward.
"My thinking is it is better they try to do so with their clubs before becoming part of the Lions squad.
"At their very best and fully fit, all three would have made the squad. And their absence from today's list does not necessarily rule them out from the tour. I have spoken to the players, and they agree with this approach."
As expected, Ireland's Brian O'Driscoll has been appointed captain, and there are places for England World Cup stalwarts Neil Back, Lawrence Dallaglio and Richard Hill.
Leicester flanker Back, 36, becomes the oldest player in Lions history selected for an original tour squad.
Several young England prospects have also received the nod, including Leicester centre Ollie Smith, Sale front-row forwards Andy Titterrell and Andrew Sheridan and Bath prop Matt Stevens.
O'Driscoll heads a strong Irish contingent, while RBS 6 Nations champions Wales have 10 Lions, but struggling Scotland are confined to scrum-half Chris Cusiter, hooker Gordon Bulloch and back-row forward Simon Taylor.
The Lions leave for New Zealand on May 25, with the first Test against New Zealand scheduled for Christchurch a month later.
British and Irish Lions squad to tour New Zealand this summer:
Backs: I Balshaw (England and Leeds), G Murphy (Ireland and Leinster), J Robinson (England and Sale Sharks), G Thomas (Wales and Toulouse), S Williams (Wales and Neath-Swansea Ospreys), J Lewsey (England and Wasps), S Horgan (Ireland and Leinster), D Hickie (Ireland and Leinster), T Shanklin (Wales and Cardiff Blues), B O'Driscoll (Ireland and Leinster, capt), G Henson (Wales and Neath-Swansea Ospreys), O Smith (England and Leicester), G D'Arcy (Ireland and Leinster), W Greenwood (England and Harlequins), S Jones (Wales and Clermont Auvergne), R O'Gara (Ireland and Munster), C Hodgson (England and Sale Sharks), M Dawson (England and Wasps), D Peel (Wales and Llanelli Scarlets), G Cooper (Wales and Newport Gwent Dragons), C Cusiter (Scotland and Borders).
G Rowntree (England and Leicester), A Sheridan (England and Sale Sharks), M Stevens (England and Bath), G Jenkins (Wales and Cardiff Blues), J White (England and Leicester), J Hayes (Ireland and Munster), G Bulloch (Scotland and Glasgow), S Thompson (England and Northampton), A Titterrell (England and Sale Sharks), S Byrne (Ireland and Leinster), D Grewcock (England and Bath), B Kay (England and Leicester), M O'Kelly (Ireland and Leinster), D O'Callaghan (Ireland and Munster), P O'Connell (Ireland and Munster), M Owen (Wales and Newport Gwent Dragons), R Hill (England and Saracens), N Back (England and Leicester), M Williams (Wales and Cardiff Blues), L Moody (England and Leicester), S Taylor (Scotland and Edinburgh), M Corry (England and Leicester), L Dallaglio (England and Wasps)
Speaking to the local media this week, Richard Hibbard expressed his excitement ahead of the West Country derby and that there is an extra spring in the step of the Cherry and Whites ahead of the tough challenge.
With such a short turnaround between the two games, and Gloucester looking to reintegrate several players into the line-up after the Autumn international series, Tom Savage told GRTV that the Cherry and Whites will keep changes to a minimum for the European Rugby Challenge Cup game in Zebre on Saturday.
Gloucester travel to Italy this weekend to take on Zebre in Round Four of the European Rugby Challenge Cup. The Cherry and Whites defeated the Italian side 35-10 last Sunday, but Director of Rugby David Humphreys is looking for an improved performance in the return fixture.
On a tough evening at Kingsholm, Gloucester could escape neither a suffocating Bath defence, nor the whistle of referee Greg Garner as six penalties and a penalty try paved the way to a 39-16 win for the visitors.
Gloucester Rugby welcome Sione Kalamafoni, Greig Laidlaw and Tom Palmer back to the starting line up and feature David Halaifonua in a matchday squad for the first time, as they resume Aviva Premiership action against Bath Rugby at Kingsholm on Saturday (kick off 5:30pm).