Despite injuries to Duncan McRae and Jack Adams, Dean Ryan has no fresh concerns but may decide to leave out Phil Vickery, who has a shoulder injury, to give him more time to recover.
But he is expected to name a strong side tomorrow that could include James Forrester and Alex Brown, who were this week named in the England squad.
Adam Balding, Gloucester's skipper, said: "Gloucester are taking this competition very seriously because if you win there is a route into the Heineken Cup and that is where we want to be. For us to win this competition and get into Europe would be a fantastic achievement.
"Playing the Ospreys will be a tough opener. I don't know that much about their side but it is pretty obvious it is going to be a difficult game. We will be putting everything into training this week to make sure we keep our unbeaten record this season. We have had a good start to the season and we want to keep that momentum going."
Tickets are still available for the contest and the ticket office will remain open on Thursday night until 6.30pm to give supporters the chance to take advantage of the early purchase scheme, that allows £2 per purchase.
The ticket office will also be open on Saturday morning at 10am.
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