Gloucester are set to name a strong side when they begin their Powergen Cup campaign against the Ospreys on Saturday (2.30pm).
Despite injuries to Duncan McRae and Jack Adams, Dean Ryan has no fresh concerns but may decide to leave out Phil Vickery, who has a shoulder injury, to give him more time to recover.
But he is expected to name a strong side tomorrow that could include James Forrester and Alex Brown, who were this week named in the England squad.
Adam Balding, Gloucester's skipper, said: "Gloucester are taking this competition very seriously because if you win there is a route into the Heineken Cup and that is where we want to be. For us to win this competition and get into Europe would be a fantastic achievement.
"Playing the Ospreys will be a tough opener. I don't know that much about their side but it is pretty obvious it is going to be a difficult game. We will be putting everything into training this week to make sure we keep our unbeaten record this season. We have had a good start to the season and we want to keep that momentum going."
Tickets are still available for the contest and the ticket office will remain open on Thursday night until 6.30pm to give supporters the chance to take advantage of the early purchase scheme, that allows £2 per purchase.
The ticket office will also be open on Saturday morning at 10am.
Speaking to the local media this week, John Afoa described the hard work that the Cherry and White have been putting in and that he is fighting fit ready for Saturday's European Rugby Challenge Cup semi final clash with Exeter Chiefs.
Gloucester Rugby face Exeter Chiefs this Saturday in the European Rugby Challenge Cup semi-final, and Charlie Sharples, who starts at full back is relishing the challenge of the big Kingsholm occasion.
Gloucester meet the Exeter Chiefs for the fourth time in competitive action this weekend, this time in the semi-final of the European Rugby Challenge Cup. Director of Rugby David Humphreys told the local media this week that this is the sort of game that Gloucester want to be involved in on a regular basis and that he and his team are looking forward to it.
Following the 29-26 loss to Harlequins, club captain Billy Twelvetrees spoke of his disappointment that the Cherry and Whites were unable to cash in on the home side's indiscipline and lose a lead on Saturday afternoon.
Number eight Gareth Evans admitted to a collective feeling of frustration as Gloucester lost narrowly at Harlequins on Saturday, but insisted that there are plenty of positives to draw upon ahead of next Saturday's European Rugby Challenge Cup semi-final at home to Exeter.
Gloucester travel to the Stoop on Saturday afternoon to take on Harlequins in the Aviva Premiership. The Cherry and Whites make just two changes from last weekend's win over Connacht as they seek consistency and continuity in their play as Nick Walshe told the local media this week.
Gloucester Rugby qualified for their first European final since 2006 as they defeated the in-form Exeter Chiefs by 30 points to 19 in a thrilling semi-final at Kingsholm on Saturday evening. The club photographer caught the best of the action.
Recent meetings between these two teams have often been thrillers, and this was no exception. It went down to the final few minutes, but Gloucester produced the goods when it mattered to qualify for the European Rugby Challenge Cup Final, beating Exeter 30-19.
Supporters who are still to purchase tickets for Saturday's European Rugby Challenge Cup semi-final at home to Exeter Chiefs at Kingsholm (kick off 7:45pm) are reminded that Early Bird prices are only valid until 6:30pm on Thursday evening.