Tickets for Gloucester's Zurich Wild Card final against Saracens will go on general sale to all supporters from 9am on Wednesday from the Kingsholm ticket office.
Tickets have been on sale to season ticket holders since 10am on Monday, and will now go on general sale to all supporters on Wednesday morning.
The tickets are available from the ticket hotline on 0871 871 8781 and from the ticket office at the Kingsholm ground.
A record crowd is expected at the Zurich Premiership Final at Twickenham on Saturday 14 May.
Gloucester's Wild Card clash with Saracens is followed by the Zurich Premiership final between Martin Johnson's Leicester Tigers and Lawrence Dallaglio's London Wasps at 3.30pm.
The last 2,000 tickets went on sale via Ticketmaster on 0870 902 0000 or www.ticketmaster.co.uk on Monday afternoon. The only other tickets are available through the competing clubs, including Gloucester's ticket office, whilst their allocations last.
Supporters purchasing tickets over the phone from Gloucester's ticket office will be asked to collect them, or offered the option to have them sent recorded delivery to ensure receipt of the tickets in time, as duplicates can't be reprinted.
The tickets available include: North Stand Lower (blocks 24, 25 and 27): Adults £25, Juniors £12
East Stand Lower (blocks 28 and 29): Adults £40, Juniors £20
East Stand Middle (blocks 126 and 127): Adults £40, Juniors £20
West Stand Lower (blocks 9 and 10): Adults £40, Juniors £20
West Stand Middle (block 102): Adults £40, Juniors £20
Ticket office opening hours: Monday 10.00 am to 7.30 pm
Tuesday 9.00 am to 7.30 pm
Wednesday 9.00 am to 7.30 pm
Thursday 9.00 am to 3.00 pm
All tickets have to be returned to Twickenham overnight on Thursday. The ticket office will be open on Saturday for collections ONLY (7.00 am to 8.30 am - as coaches will depart at 8.30 am).
Travel tickets for the supporters' coaches going to Twickenham are also on sale from the ticket office. The buses leave the Kingsholm car park at 8.30am on Saturday morning, and the return journey will depart between 6pm-7pm from Twickenham.
As Gloucester prepare for their 39th game of the season, against Bordeaux-Begles at Sixways on Sunday, David Humphreys told the local media that this week has been all about getting the players mentally and physically ready after a tough game against Connacht last Sunday.
Speaking to GRTV after the 40-32 victory over Connacht on Sunday, skipper Billy Twelvetrees explained that Gloucester had had to learn to be patient and cut out the errors to earn the win and progress to next weekend's final play-off against Bordeaux-Begles.
Jonny May's superb late score capped a thrilling 40-32 European play-off win over Connacht on Sunday. The winger spoke afterwards of his team's character and spirit as he looked ahead to one more week of rugby.
Speaking to GRTV this afternoon, Director of Rugby David Humphreys hailed the 'never say die' attitude of the team, after despite not being at their best, fighting back to take a 40-32 win after extra time over Connacht.
Jonny May continues on the wing on Sunday as Connacht come to town, and the England wing explains to the local media this week that he is delighted with his form and is looking forward to taking on Pat Lam's men.
Premiership Rugby have this morning confirmed the order of fixtures for the 2015/16 season, and Gloucester Rugby will start their campaign at Kingston Park, taking on Newcastle Falcons on Friday 16 October, 19:45.