Tickets for Gloucester's Zurich Wild Card final against Saracens will go on general sale to all supporters from 9am on Wednesday from the Kingsholm ticket office.
Tickets have been on sale to season ticket holders since 10am on Monday, and will now go on general sale to all supporters on Wednesday morning.
The tickets are available from the ticket hotline on 0871 871 8781 and from the ticket office at the Kingsholm ground.
A record crowd is expected at the Zurich Premiership Final at Twickenham on Saturday 14 May.
Gloucester's Wild Card clash with Saracens is followed by the Zurich Premiership final between Martin Johnson's Leicester Tigers and Lawrence Dallaglio's London Wasps at 3.30pm.
The last 2,000 tickets went on sale via Ticketmaster on 0870 902 0000 or www.ticketmaster.co.uk on Monday afternoon. The only other tickets are available through the competing clubs, including Gloucester's ticket office, whilst their allocations last.
Supporters purchasing tickets over the phone from Gloucester's ticket office will be asked to collect them, or offered the option to have them sent recorded delivery to ensure receipt of the tickets in time, as duplicates can't be reprinted.
The tickets available include: North Stand Lower (blocks 24, 25 and 27): Adults £25, Juniors £12
East Stand Lower (blocks 28 and 29): Adults £40, Juniors £20
East Stand Middle (blocks 126 and 127): Adults £40, Juniors £20
West Stand Lower (blocks 9 and 10): Adults £40, Juniors £20
West Stand Middle (block 102): Adults £40, Juniors £20
Ticket office opening hours: Monday 10.00 am to 7.30 pm
Tuesday 9.00 am to 7.30 pm
Wednesday 9.00 am to 7.30 pm
Thursday 9.00 am to 3.00 pm
All tickets have to be returned to Twickenham overnight on Thursday. The ticket office will be open on Saturday for collections ONLY (7.00 am to 8.30 am - as coaches will depart at 8.30 am).
Travel tickets for the supporters' coaches going to Twickenham are also on sale from the ticket office. The buses leave the Kingsholm car park at 8.30am on Saturday morning, and the return journey will depart between 6pm-7pm from Twickenham.
Fly-half Billy Burns was named Man of the Match after the 35-14 European Rugby Challenge Cup victory over Stade Rochelais on Thursday evening, and was full of praise for all his team mates for the win after a short turnaround.
Gloucester have been in good form in recent weeks, but Backs Coach Tim Taylor knows that the Cherry and Whites will have to be at their best on Thursday evening (kick off 7:45pm) against a Stade Rochelais side flying high this season.
Speaking to GRTV after the bonus point win over Bristol, back row Lewis Ludlow revealed that despite a tricky first half, Gloucester backed their skills to come through, a faith that was evident in a strong second half showing.
Willi Heinz scored Gloucester's second try as the Cherry and Whites overturned a disappointing first 40 minutes and 0-6 half time deficit, to earn a 26-18 Aviva Premiership victory over Bristol Rugby on Saturday.
Gloucester's much changed side had to overcome conceding an early try on Thursday evening, but responded in style to down Stade Rochelais by 35 points to 14 in the European Rugby Challenge Cup and set up a mouthwatering return fixture next weekend.
After taking 11 points from their last three games in the Aviva Premiership, including bonus point wins against Wasps and Bristol, Gloucester will be looking to continue their good form when they welcome La Rochelle to Kingsholm.