Dan Robson featured in this tournament last year and plays his first rugby since representing England U20s in the Junior World Cup.
Robson is joined by fellow Academy players Gareth Evans and Rob Langley while Ian Clark is a new recruit to the Academy this season.
Waylon Gasson and Mark Woodrow are both familiar figures to followers of South West rugby while Hartpury RFC contribute several members of the squad.
Gloucester Rugby squad:
Olly Robinson, Reuben Haile, Gareth Evans, Rob Langley, Kobus du Rand, Waylon Gasson, Mark Woodrow, Ian Clark, Dan Robson, Ryan Brend, Fred Burdon, Kyle Palm