They also had to overcome the late withdrawal of Lesley Vainikolo with Dan Robson taking over on the right wing and club video analyst Rob Thirlby receiving a call up to the bench.
Wasps, clearly looking to put width into their game, got both their wingers into the game early, with Chris Mayor and Elliot Daly getting plenty of ball in the first few minutes.
After 13 minutes Wasps scored a cracking opening try after Mayor made a great break through the heart of the Gloucester defence and quick hands released Daly who had too much pace out wide as he finished the chance very well. Davis missed the conversion.
Gloucester hit right back with a try of their own minutes later when Dan Robson broke out wide and collected his own neat kick to go over under the posts. Tim Taylor converted and it was 5-7 after 17 minutes.
Taylor then added a penalty to push the lead out to five and the fly half was at the heart of the next Gloucester score as he made a break and offloaded to Jonny May who dived over under the posts. The conversion made it Wasps 5 Gloucester 17.
Davis replied with a penalty for Wasps as the game continued at good pace with both sides looking to attack at will.
Wasps captain for the night Sam Jones was on hand to prevent a breakaway by May with a solid tackle 30 minutes in but Gloucester began to get the upper hand in the game as they controlled possession.
Their work was rewarded when winger Ian Clarke was on the end of some excellent handling to score just before halftime with Taylor's kick making it 8-24 with just two minutes to go to halftime.
However, Joe Burton grabbed a try just before the break with a quickly taken tap seeing him sidestep the defence and race over from 40 yards for a wonderful individual score leaving it 15-24 at halftime.
Both sides made a raft of changes at halftime and Gloucester were on the scoreboard first with a straightforward penalty from Taylor.
Wasps then enjoyed a good period of possession with Gus Jones, the Eton schoolboy who had come on for Sam Jones, making a lovely break from the back of a lineout and the pressure forced a breakdown infringement from Shaun Knight who was yellow carded.
Wasps efforts paid off when Jonah Holmes then sneaked over for a try under the posts after sustained pressure in attack and Davis converted to make it a five point game 22-27.
The home side were then forced to scramble in defence as a mix-up in midfield saw the ball bounce loose and Gloucester were suddenly had a five metre scrum having hacked the ball through well. The resultant penalty saw Rory Teague restore a two score lead as they went 30-22 up with fifteen minutes to go.
Teague then put the game beyond reach with a penalty in the dying minutes though Wasps showed plenty of spirit as the game closed out with Gus Jones and Lewis Thiede involved in a fine attacking burst in the last minute but it was too little too late.
London Wasps A:
Jack Wallace, Charlie Ingall, Elliot Daly, Alex Cheeseman, Chris Mayor, Ryan Davis, Charlie Davies, Bob Baker, Nathan Morris, Jeremy Castex, Sam McDonald, Dan Smith, Sam Jones, James Harris, Joe Burton.
Replacements: Federico Rodriguez (for Morris 40), Alec Hepburn (for Burton 44), Lewis Thiede (for Castex 62), Dave Shotton (for McDonald 30), Gus Jones (for S Jones 40), Jonah Holmes (for Davies 44), Will Robinson (for Davis 62), Chris Bishay (for Daly 33)
Jonny May; Dan Robson, Ben Mercer, Ryan Mills, Ian Clark; Tim Taylor, Nick Runciman; Dan Murphy, Matias Cortese, Dario Chistolini; Will Graulich, Dave Markham; Matt Cox, Andy Hazell, Gareth Evans
Yann Thomas (for Murphy 40), Koree Britton (for Cortese 40), Shaun Knight (for Chistolini), Rob Langley, Louis Spencer (for Cox 76) Sandro Nijaradze (for Runciman), Rory Teague (for Taylor 52), Rob Thirlby (for Clarke 76)