Uncapped Gloucester prop Nick Wood has been named amongst the replacements for England for Saturday's clash with the Barbarians.
Wood's selection means that he could potentially be lining up against Gloucester team mate Greg Somerville who starts at tight head for the Barbarians.
Lewis Moody is back in the England side after a 15 month absence and six players will make their first starts.
World Cup winner Moody, a Guinness Premiership winner and Heineken Cup finalist with Leicester Tigers this season, hasn't featured for England since the opening game of the 2008 RBS 6 Nations against Wales.
The 30-year-old lines up in the back-row with Nick Easter and uncapped Harlequins flanker Chris Robshaw, the Guinness Premiership's Player of the Season.
The five other players making their first start are Dylan Hartley and Ben Foden, who were European Challenge Cup winners with Northampton Saints last week, and Harlequins centre Jordan Turner-Hall, Bath Rugby wing Matt Banahan and Newcastle Falcons prop David Wilson.
England team manager Martin Johnson also calls on captain Steve Borthwick (Saracens) and Delon Armitage (London Irish), who played throughout the 2009 RBS 6 Nations campaign, while hooker Steve Thompson and flanker James Haskell are among the replacements.
The game will be England's final appearance of the 2008/09 season at Twickenham before they prepare for their two Test series against Argentina at Old Trafford on June 6 and Salta, Argentina, on June 13.
Delon Armitage (London Irish); Ben Foden (Northampton Saints), Jamie Noon (Newcastle Falcons), Jordan Turner-Hall (Harlequins)*, Matt Banahan (Bath Rugby)*; Andy Goode (CA Brive), Danny Care (Harlequins); Tim Payne (London Wasps), Dylan Hartley (Northampton Saints), David Wilson (Newcastle Falcons)*; Steve Borthwick (Saracens, captain), Louis Deacon (Leicester Tigers); Chris Robshaw (Harlequins)*, Lewis Moody (Leicester Tigers)
8 Nick Easter (Harlequins)
Steve Thompson (CA Brive), Nick Wood (Gloucester Rugby)*, Chris Jones (Sale Sharks), Steffon Armitage (London Irish), James Haskell (London Wasps), Paul Hodgson (London Irish), Tom May (Newcastle Falcons)*
* Uncapped players (note that this is not a Test match and caps are not awarded for this game).
Dan Murphy was part of a Gloucester pack which took a grip as the Sale game progressed and, ultimately, were rewarded with a late penalty try. The loose head prop says the team's improved scrummaging is down to a lot of hard work over the summer.
Gloucester clinched their first Aviva Premiership win of the season on Saturday, 34-27 over Sale Sharks, and scrum half Greig Laidlaw felt that the Kingsholm crowd played a massive part as they spurred the team on.
David Humphreys spoke to Gloucester Rugby TV after the 34-27 win over Sale Sharks at Kingsholm. The Director of Rugby praised the Kingsholm crowd for spurring the team on to a super second half comeback.
Speaking to Gloucester Rugby TV this week, Sione Kalamafoni is expecting a tough, physical battle against Sale Sharks as the Cherry and Whites return to Kingsholm for Round Two of the Aviva Premiership
Previewing this weekend's Aviva Premiership fixture at home to Sale Sharks with the local media, Director of Media David Humphreys admitted he's looking forward to the team's first competitive outing in front of their own supporters.
Following the announcement of the 2014/15 LV= Cup fixtures on July 25, the Welsh Regions, WRU, Premiership Rugby , RFU and LV= Organising Committee have come to an agreement regarding the arrangement of Round 1 fixtures for the Welsh Regions, which clash with the Guinness Pro 12 Round 7.
Gloucester Rugby supporters will get a unique opportunity to see two of the teams who will be playing in Gloucester in Rugby World Cup 2015, when Tonga take on the USA at Kingsholm on Saturday November 15th.
Harlequins' intriguing trip to Kingsholm kicks off another bumper set of live Aviva Premiership Rugby matches on BT Sport. The 2012 Aviva Premiership Rugby Champions will now travel to face David Humphreys' Gloucester Rugby side on Friday 14 November for a 7.45pm kick-off.