Head coach Mark Mapletoft returns to his former club when the tournament kicks off against Wales on the evening of Friday, February 5, and his side will be back at Kingsholm to face Ireland on Friday, February 26.
The side's last visit to Gloucester was in March 2008, when England completed the Under 20 Six Nations Grand Slam by beating Ireland 41-13.
Mapletoft appeared as a fly half and full back for the Westcountry club, scoring 1,064 points in his 87 league and cup appearances for the club.
He said: "I really enjoyed my spell as a player at Gloucester and a good couple of games as a coach with the corresponding fixtures two years ago.
"Once we knew they were interested in hosting the games we were keen to go back there. There's always a good atmosphere there to play in, the backing for young players is very strong and off the field the set-up is very professional. They'll do anything to help you out and we have a good relationship with them at every level.
"We're under no illusions that these will be hard games, starting with a big test against Wales, who fielded lots of young lads last year under Phil Davies and will be a much tougher task with that experience under their belts."
The host club have five players in the current Under 20 Elite Player Squad. Prop Shaun Knight, lock Danny Wright, scrum half Jordi Pasqualin and fly half Freddie Burns were all capped at Under 20 level earlier this year and centre Jonny May was promoted to the squad at the start of the current season.
Knight appeared at last summer's IRB Toshiba Junior World Championship in Tokyo, where England beat Japan, Scotland, Samoa and South Africa to reach a second successive global final.
"All five Gloucester lads have a real added incentive to train well to try and get a place in the team on their home ground in front of a big local crowd," Mapletoft added.
Two of the venues for England's away games have also been confirmed. They play Italy in San Dona Di Piave on February 12 and France in St Nazaire on March 19.
RBS U20 Six Nations 2010 fixtures
February 5: England U20 v Wales U20 (Gloucester)
February 12: Italy U20 v England U20 (San Dona Di Piave)
February 26: England U20 v Ireland U20 (Gloucester)
March 12: Scotland U20 v England U20 (TBC)
March 19: France U20 v England U20 (St Nazaire)
Kick off times to be confirmed
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