The winner of the game which kicks off at Dry Leas, the home of Henley Hawks, at 7:30pm will see the winner progress to take on Northampton Saints in the final.
Gloucester qualified for the knock out stages by finishing top of Group 1, which also featured Sale Sharks, Saracens and Leicester Tigers, winning four games from six.
The side features a mixture of Academy prospects and first team experience provided by the likes of Jack Adams, Gareth Cooper and Adam Eustace.
Tickets for the game will be available on the night priced at £5 for adults and £3 for concessions.
Gloucester United
Tristan Roberts; Dan Norton, Henry Trinder, Jack Adams, Charlie Sharples; Freddie Burns, Gareth Cooper; Jack Forster, Adrian Duncan, David Young; Danny Wright, Adam Eustace; Andries Pretorious, Guy Thompson, Dan Williams
Replacements:
Chris Hall, Yann Thomas, Richard Jenkins, Will Foden, Ollie Winterbottom, Jonny May, Danny Pointon
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