However, there will also be a sprinkling of first team experience on display including Matt Cox and Martyn Thomas, who have represented England and Wales respectively at Sevens, as well as Akapusi Qera and Koree Britton.
Thirlby has also utilised his Cornish connections to bring in Rhodri McAtee, a member of the victorious Wales Sevens squad at the 2009 Rugby World Cup Sevens.
McAtee has recently been appointed player-coach at Redruth, where Thirlby's fly half brother Paul plays his club rugby.
Also of note is the presence of Gareth Evans in the squad. Back row forward Evans has been a member of the Gloucester squad in all of the four JP Morgan Sevens Series to date.
Thirlby, who represented England in RWC Sevens 2001 and 2005 and who is now the club's Video Analyst, is looking forward to the evening.
"We've tried to name a squad which combines some of our best young prospects with a number of first team squad players.
"I think the JP Morgan 7s Series a really good concept. It's good for players to have a good run out and to showcase the best talent coming through for the longer format.
"We've got a number of really talented, skilful players on display and it's a great opportunity for them to show what they can do on a big stage.
"Last summer saw a great evening of Sevens rugby at Kingsholm. The Gloucester crowd really got behind us and it made for an amazing atmosphere on the night. Hopefully Thursday evening will be another big night."
Tom Ailes, Koree Britton, Reece Boughton, Billy Burns, Drew Cheshire, Matt Cox, Gareth Evans, Lewis Ludlow, Rhodri McAtee, Akapusi Qera, Steph Reynolds, Martyn Thomas