There are first starts in Cherry and White for new front row additions Richard Hibbard and John Afoa alongside Dan Murphy, who impressed in the front row against Yorkshire Carnegie last weekend.
James Hudson and Tom Savage bring reliability in the second row with the familiar back row trio of Sione Kalamafoni alongside England duo Matt Kvesic and Ben Morgan, who get their first run outs of the summer.
At half back, International pair Greig Laidlaw and James Hook will be looking to pick up where they left off last weekend, as they start together for the first time.
The Cherry and Whites welcome back Jonny May, Henry Trinder and club captain Billy Twelvetrees in the back line.
Last year's player of the season Rob Cook is joined in the back three by Charlie Sharples.
There is an extended bench at Gloucester's disposal with plenty of the squad looking to impress.
Tickets are still on sale for Saturday's game, visit www.gloucesterrugby.co.uk/tickets or call the ticket office on 0871 871 8781.
If you cannot make the game in person, you can follow the game from Kingsholm live on the club's twitter feed, and we will also be revealing an exciting new way in which to follow the action.
Gloucester Rugby to face Munster Rugby at Kingsholm on Saturday (3pm kick off)
Rob Cook, Charlie Sharples, Henry Trinder, Billy Twelvetrees (capt), Jonny May, James Hook, Greig Laidlaw; Dan Murphy, Richard Hibbard, John Afoa; Tom Savage, James Hudson; Sione Kalamafoni, Matt Kvesic, Ben Morgan.
Yann Thomas, Darren Dawidiuk, Shaun Knight; Elliott Stooke, Tom Palmer, Jacob Rowan, Ross Moriarty; Callum Braley, Aled Thomas; Shane Monahan, Mark Atkinson, Tom Isaacs, Henry Purdy, Steve McColl
45 - 8
HT: 10 - 0
23rd August 2014