Koree Britton and Ian Clark will make first European starts, but the benefit of squad rotation in recent weeks pays off with the majority of those starting on Thursday evening having seen first team action this season.
And, as Nigel Davies stressed to the media this week before departure, the onus is on this group of players to keep Gloucester's good run of form going.
"Momentum is very important, to continue winning and developing and building our game is really important.
"We've rotated the squad in the Premiership fixtures already but it's important that we give an opportunity to some others."
"But, we're expecting no less from these guys. They have to step up to the plate and they've to take our game forward. We have to push on and get better and stronger."
Mont de Marsan may sit at the foot of the French Top 14 table, but Gloucester's experience in Agen two years ago means that nothing is being taken for granted and Davies confirmed that they've fully analysed the French side.
"They're very French - very big, very unpredictable but very capable. If you go out and play French teams and you're accurate and precise then you have every chance.
"If you don't, and it becomes loose and dominated by errors, then they tend to win those games. It's a big challenge for us, a different type of challenge but an interesting one."
Gloucester Rugby:
Martyn Thomas; Charlie Sharples, Tim Molenaar, Mike Tindall (capt), Ian Clark; Tim Taylor, Dave Lewis; Yann Thomas, Koree Britton, Shaun Knight; Peter Buxton, Will James; Matt Cox, Andy Hazell, Sione Kalamafoni
Replacements:
Tommaso d'Apice, Nick Wood, Dario Chistolini, Elliott Stooke, Gareth Evans, Jimmy Cowan, Henry Trinder, Billy Twelvetrees
