Martyn Thomas (c)
A Steph Reynolds double in the final caught the eye and after Rhodri McAtee ghosted home a late Nevaro Codlin score put an end to a spirited Tigers comeback.
Reynolds exploded into life in the final as his pace and elusive running was too hot to handle and the youngster created space and opportunities for his team mates who more than often took advantage.
The final finished 24-17 and the celebrations began as the huge hoards of travelling Cherry and White fans revelled on the terraces at The Rec.
It had taken a dramatic late Andrew Bulumakau try to secure Gloucester's place in the final after defeating Saracens and the quick turnaround between matches was not evident as they raced into a 17-0 half-time lead.
Gloucester fans could've been forgiven for fearing that lightning would strike twice after London Irish's dramatic comeback in last year's final because the Tigers upped their game a gear and got back to within a score.
The Cherry and Whites were smarter and were not to be denied on the night and after a period of ball retention to quash the Tigers' momentum, Drew Cheshire fed Nevaro Codlin to race home and the sea of Cherry and White were toasting a late victory.
Gloucester took on Newcastle in the evening's second game after Leicester had cruised past Worcester.
With the Falcons looking dangerous early on it was the star of Gloucester's pool stages Steph Reynolds who crossed following neat build-up play and a lung bursting run from Matt Cox. Rhodri McAtee and Billy Burns drew the defence and Reynolds darted past the cover to dot down.
From then on it was the Cherry and Whites side that dominated the proceedings.
The much larger and physical Falcons side spoilt several chances and ultimately it was Olly Morgan's young side that took chances when they came. Early on in the Second half, Gloucester built from deep and Apo Satala fed Gareth Evans who cleverly stepped inside the covering duo to score.
As Falcons scrapped to remain in the game, Andrew Bulumakau was seemingly going nowhere until a clever change of direction and pace took him away from the cover, to put the game to bed.
It was clinical and impressive from Gloucester who had a bit of time to wait and survey the opposition as Leicester made it 2/2 beating Harlequins with the final play.
In the final pool match, in front of a great atmosphere in The Rec, a last gasp Andrew Bulumakau try sent the Gloucester fans in jubilation as he darted away down the touchline from Akapusi Qera's clever offload.
Saracens had been leading and looked set to take on Leicester in the evening's showpiece event after a Ben Ransom double had seen them lead 14-10 as the clock hit zero. Tries from Steph Reynolds and skipper Martyn Thomas had kept Gloucester in control of the game but the conversions from Sam Stanley looked to be the difference.
But it was the Drybrook RFC winger who crossed for a dramatic ending.
It was a quick turnaround for the final, Leicester having an hour's rest as opposed to Gloucester's mere 10 minutes but as it happened the Cherry and Whites had a bit too much nouse for a very young but exciting Leicester side.
It was a fantastic evening for everyone connected with Gloucester Rugby and a perfect start to the next campaign.