With another LV= Cup game against Newcastle at home on Saturday (kick off 4:30pm), and then the return to Aviva Premiership action, there's an opportunity for all concerned to impress.
The starting line up features five full internationals in Andy Hazell, Jimmy Cowan, Jonny May, Mike Tindall and James Simpson-Daniel with another in Charlie Sharples on the bench.
Tickets for Tuesday's clash against Japan are still available.
To purchase, please call 0871 871 8781 (option 2), visit www.gloucesterrugby.co.uk/tickets or visit the Ticket Office at Kingsholm.
Gloucester Rugby:
Rob Cook; James Simpson-Daniel, Henry Trinder, Mike Tindall, Jonny May; Ryan Mills, Jimmy Cowan; Yann Thomas, Darren Dawidiuk, Rupert Harden; Elliott Stooke, James Hudson; Tom Savage (capt), Andy Hazell, Matt Cox
Replacements:
Koree Britton, James Gibbons, Shaun Knight, Lewis Ludlow, Ross Moriarty, Dan Robson, Billy Burns, Charlie Sharples