Ryan Mills will wear the number ten shirt after recovering from a foot injury, while also returning from injury is centre Henry Trinder who forms a strong midfield partnership alongside Mike Tindall.
Tavis Knoyle and Martyn Thomas continue at scrum half and on the wing respectively, while Rob Cook and Shane Monahan return to the starting line up.
There is also a more experienced look to the pack than the one that started in West Wales last Friday.
Nick Wood is back at loose head and James Hudson returns to partner James in the second row. And there is a new back row in Sione Kalamafoni, Matt Cox and Gareth Evans.
Of interest amongst the replacements is the selection of Academy back row forward Sam Underhill, a pupil at Sir Thomas Rich's Grammar School, while Dean Close's Lloyd Evans is selected once again.
Meanwhile, former Australia U20s and Australia Sevens cap, Bill Meakes, who has been playing for Clifton in National League 2 South, will cover the outside backs.
Forwards Coach Andrew Stanley, speaking to the media this week, insisted that the Wasps game is an important one for Gloucester.
"We're missing a few, but there's still good competition for places and we think we've picked a side to represent us that is as strong as we can go.
"For us it's important that we build on the momentum that we've generated over the past couple of weeks. We'll focus on the game on Saturday, build on that and take it into the Sale game the following week.
"A very young looking side took Scarlets to the wire last weekend. They carried the baton. We have a few more senior boys coming back in on Saturday and it's important that they carry that forward."
Rob Cook; Martyn Thomas, Henry Trinder, Mike Tindall, Shane Monahan; Ryan Mills, Tavis Knoyle; Nick Wood, Koree Britton, Rupert Harden; Will James (captain), James Hudson; Sione Kalamafoni, Matt Cox, Gareth Evans
Dan George, Dan Murphy, Shaun Knight, Tom Hicks, Sam Underhill, Jimmy Cowan, Lloyd Evans, Bill Meakes