Five players will win their first caps for England in the opening game of the RBS U20 Six Nations against Wales at Gloucester on Friday night (7.45pm). Gloucester's Freddie Burns and Shaun Knight will both start with Jonny May named amongst the replacements.
Full back Tom Catterick (Newcastle Falcons), wing Sam Smith (Harlequins), lock Calum Green (Leicester Tigers), flanker Will Welch and No.8 Alex Gray (both Newcastle Falcons) are all in the starting line-up
They slot into an experienced side that contains seven survivors from last year's IRB Junior World Championship in Tokyo, where England were beaten finalists for the second year in succession.
Back in action after missing last month's 61-7 warm-up win against Cambridge University are established Guinness Premiership performers James Gaskell (Sale Sharks), Tom Homer (London Irish) and Rory Clegg (Harlequins).
Gloucester's Freddie Burns will be at fly half on his home ground with Clegg and Homer making up an exciting midfield trio.
"We're excited about the potential of this group and we've got a nice blend of young players coming into the side together with those who were involved with us last year," said head coach Mark Mapletoft.
"We want to play an exciting brand of rugby at a high tempo and they've all bought into that.
"We're under no illusions that Wales will be a really difficult test for us, though, and they continue to produce lots of talented young players. Both sides will want to play and move the ball, so it should make for a great contest at Kingsholm."
15 Tom Catterick (Newcastle Falcons)
14 Will Hurrell (Leicester Tigers)
13 Tom Homer (London Irish)
12 Rory Clegg (Harlequins)
11 Sam Smith (Harlequins)
10 Freddie Burns (Gloucester Rugby)
9 Sam Harrison (Leicester Tigers)
1 Joe Marler (Harlequins)
2 Jamie George (Saracens)
3 Shaun Knight (Gloucester Rugby)
4 Calum Green (Leicester Tigers)
5 James Gaskell (Sale Sharks)
6 Will Welch (Newcastle Falcons)
7 Jacob Rowan (Leeds Carnegie, captain)
8 Alex Gray (Newcastle Falcons)
16 Arthur Ellis (London Wasps)
17 Lee Imiolek (Sale Sharks)
18 Charlie Matthews (Harlequins)
19 Jackson Wray (Saracens)
20 Sam Stuart (Harlequins)
21 Jonathan Joseph (London Irish)
22 Jonny May (Gloucester Rugby)
Gloucester have been in good form in recent weeks, but Backs Coach Tim Taylor knows that the Cherry and Whites will have to be at their best on Thursday evening (kick off 7:45pm) against a Stade Rochelais side flying high this season.
Speaking to GRTV after the bonus point win over Bristol, back row Lewis Ludlow revealed that despite a tricky first half, Gloucester backed their skills to come through, a faith that was evident in a strong second half showing.
Willi Heinz scored Gloucester's second try as the Cherry and Whites overturned a disappointing first 40 minutes and 0-6 half time deficit, to earn a 26-18 Aviva Premiership victory over Bristol Rugby on Saturday.
It's the first Gloucester-Bristol derby at Kingsholm in over eight years this Saturday (kick off 4:30pm) and, previewing the game with the local media this week, Defence Coach Jonny Bell stressed that, with both teams in the bottom half of the table, they both need the points.
Speaking to the local media this week, Gloucester front rower John Afoa knows the challenge ahead this week against Bristol, and calls on the Cherry and Whites to make the most of home advantage as a determined squad look to bounce back from a narrow loss to Saracens.
Tom Savage speaks to GRTV and the local media as Gloucester went down by 24-20 at Saracens on Sunday afternoon. The second row was disappointed with the result, despite a strong showing from the hosts.
Speaking immediately after the game, skipper Willi Heinz thought that Gloucester had executed their game plan reasonably well and competed hard against Saracens. However, it wasn't enough but the squad will have plenty to work on this week ahead of the derby match against Bristol.
Having named an unchanged starting XV for the past three Aviva Premiership games, Gloucester Rugby ring the changes and retain only five players for Thursday's visit of Stade Rochelais in the European Rugby Challenge Cup (kick off 7:45pm).