Five players will win their first caps for England in the opening game of the RBS U20 Six Nations against Wales at Gloucester on Friday night (7.45pm). Gloucester's Freddie Burns and Shaun Knight will both start with Jonny May named amongst the replacements.
Full back Tom Catterick (Newcastle Falcons), wing Sam Smith (Harlequins), lock Calum Green (Leicester Tigers), flanker Will Welch and No.8 Alex Gray (both Newcastle Falcons) are all in the starting line-up
They slot into an experienced side that contains seven survivors from last year's IRB Junior World Championship in Tokyo, where England were beaten finalists for the second year in succession.
Back in action after missing last month's 61-7 warm-up win against Cambridge University are established Guinness Premiership performers James Gaskell (Sale Sharks), Tom Homer (London Irish) and Rory Clegg (Harlequins).
Gloucester's Freddie Burns will be at fly half on his home ground with Clegg and Homer making up an exciting midfield trio.
"We're excited about the potential of this group and we've got a nice blend of young players coming into the side together with those who were involved with us last year," said head coach Mark Mapletoft.
"We want to play an exciting brand of rugby at a high tempo and they've all bought into that.
"We're under no illusions that Wales will be a really difficult test for us, though, and they continue to produce lots of talented young players. Both sides will want to play and move the ball, so it should make for a great contest at Kingsholm."
15 Tom Catterick (Newcastle Falcons)
14 Will Hurrell (Leicester Tigers)
13 Tom Homer (London Irish)
12 Rory Clegg (Harlequins)
11 Sam Smith (Harlequins)
10 Freddie Burns (Gloucester Rugby)
9 Sam Harrison (Leicester Tigers)
1 Joe Marler (Harlequins)
2 Jamie George (Saracens)
3 Shaun Knight (Gloucester Rugby)
4 Calum Green (Leicester Tigers)
5 James Gaskell (Sale Sharks)
6 Will Welch (Newcastle Falcons)
7 Jacob Rowan (Leeds Carnegie, captain)
8 Alex Gray (Newcastle Falcons)
16 Arthur Ellis (London Wasps)
17 Lee Imiolek (Sale Sharks)
18 Charlie Matthews (Harlequins)
19 Jackson Wray (Saracens)
20 Sam Stuart (Harlequins)
21 Jonathan Joseph (London Irish)
22 Jonny May (Gloucester Rugby)
Speaking after the 55-0 win over CA Brive, Jonny May told GRTV that he's delighted that the team's hard work is starting to pay dividends but was equally happy for his friend and team mate, Charlie Sharples, on what was a big night for his fellow winger.
Speaking to the local media this week, David Humphreys insisted that Gloucester are looking forward to kicking off the new European Rugby Challenge Cup against Brive on Thursday evening, although an unfamiliar opponent and a five day turnaround will pose a challenge.
Director of Rugby David Humphreys felt that his Gloucester side put themselves in a position to put Saracens under real pressure at Allianz Park on Saturday, praising his team's commitment and defence, while admitting that a lack of accuracy hit their chances in the second half.
Speaking to the local media at Allianz Park, Tom Savage was disappointed after the defeat at Saracens, but claimed Gloucester did plenty of good things and could've taken more than a losing bonus point.