Five players will win their first caps for England in the opening game of the RBS U20 Six Nations against Wales at Gloucester on Friday night (7.45pm). Gloucester's Freddie Burns and Shaun Knight will both start with Jonny May named amongst the replacements.
Full back Tom Catterick (Newcastle Falcons), wing Sam Smith (Harlequins), lock Calum Green (Leicester Tigers), flanker Will Welch and No.8 Alex Gray (both Newcastle Falcons) are all in the starting line-up
They slot into an experienced side that contains seven survivors from last year's IRB Junior World Championship in Tokyo, where England were beaten finalists for the second year in succession.
Back in action after missing last month's 61-7 warm-up win against Cambridge University are established Guinness Premiership performers James Gaskell (Sale Sharks), Tom Homer (London Irish) and Rory Clegg (Harlequins).
Gloucester's Freddie Burns will be at fly half on his home ground with Clegg and Homer making up an exciting midfield trio.
"We're excited about the potential of this group and we've got a nice blend of young players coming into the side together with those who were involved with us last year," said head coach Mark Mapletoft.
"We want to play an exciting brand of rugby at a high tempo and they've all bought into that.
"We're under no illusions that Wales will be a really difficult test for us, though, and they continue to produce lots of talented young players. Both sides will want to play and move the ball, so it should make for a great contest at Kingsholm."
15 Tom Catterick (Newcastle Falcons)
14 Will Hurrell (Leicester Tigers)
13 Tom Homer (London Irish)
12 Rory Clegg (Harlequins)
11 Sam Smith (Harlequins)
10 Freddie Burns (Gloucester Rugby)
9 Sam Harrison (Leicester Tigers)
1 Joe Marler (Harlequins)
2 Jamie George (Saracens)
3 Shaun Knight (Gloucester Rugby)
4 Calum Green (Leicester Tigers)
5 James Gaskell (Sale Sharks)
6 Will Welch (Newcastle Falcons)
7 Jacob Rowan (Leeds Carnegie, captain)
8 Alex Gray (Newcastle Falcons)
16 Arthur Ellis (London Wasps)
17 Lee Imiolek (Sale Sharks)
18 Charlie Matthews (Harlequins)
19 Jackson Wray (Saracens)
20 Sam Stuart (Harlequins)
21 Jonathan Joseph (London Irish)
22 Jonny May (Gloucester Rugby)
Skipper Billy Twelvetrees admitted that the Gloucester squad were disappointed and frustrated after losing at home to Exeter, regretting that Gloucester's own errors had given the Chiefs the upper hand.
David Humphreys spoke to the local press after the Gloucester Rugby team to face Exeter Chiefs on Friday was announced. Henry Purdy is making his Premiership debut for Gloucester and thoroughly deserves his chance says Humphreys.
Dan Murphy was part of a Gloucester pack which took a grip as the Sale game progressed and, ultimately, were rewarded with a late penalty try. The loose head prop says the team's improved scrummaging is down to a lot of hard work over the summer.
Gloucester clinched their first Aviva Premiership win of the season on Saturday, 34-27 over Sale Sharks, and scrum half Greig Laidlaw felt that the Kingsholm crowd played a massive part as they spurred the team on.
David Humphreys spoke to Gloucester Rugby TV after the 34-27 win over Sale Sharks at Kingsholm. The Director of Rugby praised the Kingsholm crowd for spurring the team on to a super second half comeback.
Speaking to Gloucester Rugby TV this week, Sione Kalamafoni is expecting a tough, physical battle against Sale Sharks as the Cherry and Whites return to Kingsholm for Round Two of the Aviva Premiership
The message from the Gloucester Rugby Ticket Office is that tickets still remain for this evening's Aviva Premiership game against Exeter Chiefs and that there are a large number of tickets to be collected on the night.