Five players will win their first caps for England in the opening game of the RBS U20 Six Nations against Wales at Gloucester on Friday night (7.45pm). Gloucester's Freddie Burns and Shaun Knight will both start with Jonny May named amongst the replacements.
Full back Tom Catterick (Newcastle Falcons), wing Sam Smith (Harlequins), lock Calum Green (Leicester Tigers), flanker Will Welch and No.8 Alex Gray (both Newcastle Falcons) are all in the starting line-up
They slot into an experienced side that contains seven survivors from last year's IRB Junior World Championship in Tokyo, where England were beaten finalists for the second year in succession.
Back in action after missing last month's 61-7 warm-up win against Cambridge University are established Guinness Premiership performers James Gaskell (Sale Sharks), Tom Homer (London Irish) and Rory Clegg (Harlequins).
Gloucester's Freddie Burns will be at fly half on his home ground with Clegg and Homer making up an exciting midfield trio.
"We're excited about the potential of this group and we've got a nice blend of young players coming into the side together with those who were involved with us last year," said head coach Mark Mapletoft.
"We want to play an exciting brand of rugby at a high tempo and they've all bought into that.
"We're under no illusions that Wales will be a really difficult test for us, though, and they continue to produce lots of talented young players. Both sides will want to play and move the ball, so it should make for a great contest at Kingsholm."
15 Tom Catterick (Newcastle Falcons)
14 Will Hurrell (Leicester Tigers)
13 Tom Homer (London Irish)
12 Rory Clegg (Harlequins)
11 Sam Smith (Harlequins)
10 Freddie Burns (Gloucester Rugby)
9 Sam Harrison (Leicester Tigers)
1 Joe Marler (Harlequins)
2 Jamie George (Saracens)
3 Shaun Knight (Gloucester Rugby)
4 Calum Green (Leicester Tigers)
5 James Gaskell (Sale Sharks)
6 Will Welch (Newcastle Falcons)
7 Jacob Rowan (Leeds Carnegie, captain)
8 Alex Gray (Newcastle Falcons)
16 Arthur Ellis (London Wasps)
17 Lee Imiolek (Sale Sharks)
18 Charlie Matthews (Harlequins)
19 Jackson Wray (Saracens)
20 Sam Stuart (Harlequins)
21 Jonathan Joseph (London Irish)
22 Jonny May (Gloucester Rugby)
After his last ditch try clinched the 42-40 Aviva Premiership victory over Newcastle at Kingsholm on Saturday, Billy Burns told GRTV that all the Gloucester team deserve credit for hanging in there against a Falcons team who really made life tough for the Cherry and Whites.
Speaking ahead of Saturday's Aviva Premiership fixture at home to Newcastle Falcons (kick off 3pm), Director of Rugby David Humphreys emphasised the importance this weekend of Gloucester building on last week's impressive home victory against the Exeter Chiefs.
Speaking to the assembled national and local media, try-scorer Jonny May was delighted and relieved to have tasted victory on Saturday evening, and is looking forward to the European Rugby Challenge Cup final.
Tom Savage delivered a Man of the Match performance against Exeter Chiefs on his birthday on Saturday evening. He spoke to GRTV after the game about the importance of the European Rugby Challenge Cup win.
Speaking to the local media this week, John Afoa described the hard work that the Cherry and White have been putting in and that he is fighting fit ready for Saturday's European Rugby Challenge Cup semi final clash with Exeter Chiefs.
Gloucester Rugby face Exeter Chiefs this Saturday in the European Rugby Challenge Cup semi-final, and Charlie Sharples, who starts at full back is relishing the challenge of the big Kingsholm occasion.
Gloucester Rugby return to Aviva Premiership action against the Newcastle Falcons on Saturday (Kingsholm, kick off 3pm), and make several changes to the side that defeated Exeter Chiefs at home last weekend.