Powerful flanker Billy Vunipola (Harrow & London Wasps) also returns after recovering from an ankle injury sustained in the 30-28 win over Australian Schools in December.
England, captained by fly half George Ford (Rishworth & Leicester Tigers), extended their run to 14 international victories since the summer of 2007 with their 33-16 win against France at Newbury last month.
Fletcher is committed to making sweeping changes to his starting line-up to broaden the experience of his young players.
"We wanted to give a start to all the players in the games against France and Italy before playing Wales and the two Festival games against Scotland and Ireland in April," said Fletcher.
"We've made a good start to the season and while we know we've still got things to work on, I'm confident we'll iron those out over the next four games."
Those making way from the 23 on duty against France are scrum half Joel Hodgson (RGS Newcastle & Newcastle Falcons) and wing Charlie Walker (Oakham & Leicester Tigers), who are both injured, wing Marland Yarde (Whitgift & London Irish), prop Luke Cowan-Dickie (Truro Coll & Exeter Chiefs) and flanker Chris Walker (Prince Henry's GS Otley & Leeds Carnegie).
England Under 18 squad
Nick Auterac (Oaklands College & Saracens)
Dominic Barrow (Prince Henry's GS Otley & Leeds Carnegie)
Anthony Brennan (Twyford CofE & London Wasps)
Koree Britton (Ivybridge Coll & Exeter Chiefs)
Matt Kvesic (Worcester Sixth Form Coll & Worcester Warriors)
George Merrick (Whitgift & Harlequins)
Nathan Morris (Sedbergh & Newcastle Falcons)
Sam Northcote-Green (Dulwich Coll & London Wasps)
Kyle Sinckler (Epsom Coll & Harlequins)
Will Skuse (Bryanston & Bath Rugby)
Billy Vunipola (Harrow & London Wasps)
Will Spencer (St Paul's Catholic & London Irish)
Tommy Bell (Bacup and Rawtenstall GS & Sale Sharks)
Elliot Daly (Whitgift & London Wasps)
Chris Elder (RGS High Wycombe & London Wasps)
Jamie Elliott (Bedford & Northampton Saints)
George Ford (Rishworth & Leicester Tigers)
Charlie Gordon (Wellington College and London Wasps)
Mark Jennings (Lymm HS & Sale Sharks)
Ryan Mills (Hartpury Coll & Gloucester)
Ben Ransom (Tonbridge & Saracens)
Dan Robson (Hartpury Coll & Gloucester Rugby)
England Under 18 fixtures 2009/10
December 13: England U18 30 Australia Schools 28
February 21: England U18 33 France 16
March 7: Italy U18 v England U18 (Calvisano 2.30pm)
March 28: Wales U18 v England U18 (Swansea TBC)
April 2: England U18 v Ireland (Llandovery 4pm)
April 7: England U18 v Scotland (Llandovery 2pm)