"The Moseley game was arranged to give us the option of looking at players at an A-team level," said team manager Rob Burgess.
"We have decided that the home fixture against Saracens and back-to-back matches with Llanelli provide us with sufficient opportunity to give all of our senior squad a run-out, and trying to fulfil a fourth fixture would be a match too far.
"We are sorry for any inconvenience caused and look forward to welcoming Moseley to Kingsholm at some other point in the next 12 months and wish them good luck in the season ahead."
Gloucester start their pre-season fixtures next Friday with a game against Saracens at Kingsholm (7.30pm) and it is expected a number of new signings will make their debuts.
They then make the trip to Stradey Park to face Llanelli on Saturday, August 19 at 2.30pm and host the Scarlets at Kingsholm on Saturday, August 26 at 3pm.
Tickets for those games are now on sale and supporters who have already purchased tickets for the Moseley game will be refunded.
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