As well as Toulouse, Gloucester will compete against fellow Premiership side Leeds Tykes and French side Clermont Auvergne (formerly Montferrand) in the Issoire tournament.
The summer tournament takes place between 29 July and 31 July and will be part of the Club's pre-season schedule after the players return to training at the end of June.
The tournament will see two semi-finals on 29 July at 5pm and 7pm (draw to be made soon), following by a runners-up clash and the final on Sunday 31 July at 3pm and 5pm, respectively.
Issoire is a French rugby club that plays in the leagues just below the top two divisions and is 20 minutes (by car) south of Clermont Ferrand (where Clermont Auvergne are based).
Gloucester will also be taking part in the Compass Group Middlesex Sevens on Saturday 13 August.
The West Country side were runners-up in last season's event and will compete against 15 other teams for this year's trophy, including all the Premiership clubs, NEC Harlequins, Blackheath, Bedford Blues and last year's winners, the British Army.
Gloucester is scheduling a number of other pre-season fixtures that will be announced soon.
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