Reflecting its rugby heritage, Wooden Spoon teamed up with Gloucester, Bath, London Irish and Worcester rugby clubs to give participants a choice of start point.
The terrain varied depending on the route, but all routes were prepared in advance by the organisers who made safety a priority by incorporating minor roads, tow paths and sections of the Sustrans National Cycle Network.
The 50mile bike ride for the Gloucester participants took them from Kingsholm, through Upton St Leonards and up over Cranham towards Cirencester and on towards Avebury in Wiltshire and reaching the finishing line at Yatesbury in just under 5hrs.
Gary Little, Gloucester Rugby Community Manager said:
"Although it was not a serious race, it was most definitely a great challenge, the terrain from Gloucester to Wiltshire is very 'vertical' in places. Hence, it was necessary to take on board the occasional refreshment at hostelries along the way."
Each cyclist pledged to raise at least £300, so Wooden Spoon achieved its target, and sponsors Guinness, Greene King, Eat Natural - which supplied the cyclists with energy boosting snacks - and water company Highland Spring plus the glorious sunny weather helped make the event a resounding success.
The team managed to raise more than £1500 for the charity and there was a combined total of £12500 from everyone who took part in the ride.
"We would like to thank everyone for their support and kind donations. In particular a huge thank you to Mitchells cycles of Gloucester and Falcon Cycles who provided the bikes and technical support."