Gloucester Rugby will kick off their Anglo-Welsh campaign with a re-run of this year's final against Cardiff Blues.
The two sides who met at Twickenham in April have been drawn to play at Kingsholm on the weekend of November 7th.
The newly structured competition will see the 12 Guinness Premiership clubs and four Welsh Regions play over four weekends, followed by the semi-finals and finals.
Each team will be guaranteed two home and two away inter-pool fixtures, with the top teams from each pool qualifying for the knockout stages.
Terry Burwell, Rugby Football Union Tournaments and Competitions Director, said:
"We are delighted that together with Premier Rugby Ltd and the Welsh Rugby Union we have come up with a structure that will not only help players, coaches and officials develop but also provides more revenue potential for the Guinness Premiership clubs and the Welsh regions."
"There is a massive appetite for Cup rugby in England and Wales. The average attendance at pool matches over the past three seasons has been 8506 and the final averaged 60,000."
"There is plenty to look forward to with pool matches such as Gloucester Rugby against Cardiff Blues and Leicester Tigers against Newport Gwent Dragons in the first two rounds this coming season."
"We believe this new structure will give players, coaches and supporters an exciting tournament that embodies all that's best about cross-border knockout competition."
"We have a committed broadcaster in SkySports for the next five years and, together with PRL and the WRU, are actively seeking a title sponsor."
PRL Commercial Director Jon Varney said:
"When we were discussing the format of the new Anglo-Welsh tournament we tried to build on the success of the preceding one, but also take the opportunity to make some small changes to make the tournament even more attractive to clubs, supporters, viewers and sponsors."
"The financial structure will reward winning teams and that, as well as the European place on offer for an English winner, will make the tournament well worth winning."
WRU Group Chief Executive Roger Lewis said:
"There is a rich history and heritage surrounding Anglo-Welsh encounters stretching back many years."
"The Welsh Rugby Union is delighted that the RFU and PRL have secured the future of this great competition. We are all committed to seeing it flourish and grow from the strong base it has already created."
"This Cup competition has captured the imagination of fans from both Wales and England and the final this season was a great occasion."
"It is vitally important for the supporters from both our nations to get the opportunity to see games of the highest standard and this competition serves to deliver that and ensure our talented young players improve by playing against top class opposition."
WRU Head of Rugby Performance and Development, Joe Lydon, said:
"I am delighted that the great tradition of Anglo-Welsh competition will continue at the highest level."
"We have jointly championed this tournament because it will develop the support base for our national sport and help players achieve their full potential and challenge for international honours with Wales."
"The financial benefits are clear and it will provide our four Regions with a proven structure within which to test their progress."
"A lot of hard work has gone into ensuring this competition continues and we now have a structure which we can build on in the years ahead.
"My remit involves the whole of Welsh rugby and I know it is vitally important that our young players have meaningful competitions to set their sights on to maintain the commitment they need to reach the top."
Note to desks
* The Anglo-Welsh Cup is a 16-team competition involving the 12 Guinness Premiership clubs and 4 Welsh regions.
* Played in four pools of four with three Guinness Premiership clubs and one Welsh Region in each pool.
Pool 3 Harlequins
Pool 4 Bath Rugby
* Teams in Pool 1 play teams in Pool 4 and teams in Pool 2 play teams in Pool 3.
* Each team guaranteed four pool matches - two home and two away - with the top teams from each pool qualifying for the semi finals.
* Teams have been seeded depending on their finish in last season's Guinness Premiership and Magners League and best efforts have been made to avoid fixture duplication with both leagues, as well as the Heineken Cup, in 2009-10.
Round 1 (weekend of November 7, 2009)
Leeds Carnegie v Leicester Tigers
Newport Gwent Dragons v Sale Sharks
Saracens v Bath Rugby
Ospreys v Northampton Saints
Gloucester Rugby v Blues
London Irish v London Wasps
Worcester Warriors v Newcastle Falcons
Harlequins v Scarlets
Round 2 (weekend of November 14, 2009)
Leicester Tigers v Newport Gwent Dragons
Sale Sharks v Leeds Carnegie
Bath Rugby v Ospreys
Northampton Saints v Saracens
Cardiff Blues v London Irish
London Wasps v Gloucester Rugby
Newcastle Falcons v Harlequins
Scarlets v Worcester Warriors
Round 3 (weekend of January 30, 2010)
Leicester Tigers v Bath Rugby
Sale Sharks v Northampton Saints
Newport Gwent Dragons v Ospreys
Leeds Carnegie v Saracens
London Wasps v Scarlets
Cardiff Blues v Newcastle Falcons
Gloucester Rugby v Worcester Warriors
London Irish v Harlequins
Round 4 (weekend of February 6, 2010)
Northampton Saints v Leicester Tigers
Bath Rugby v Sale Sharks
Ospreys v Leeds Carnegie
Saracens v Newport Gwent Dragons
Scarlets v Cardiff Blues
Newcastle Falcons v London Wasps
Worcester Warriors v London Irish
Harlequins v Gloucester
Semi finals (weekend of March 13, 2010, venue TBC)
Final (weekend of March 20, 2010, venue TBC)
* If the winner of the competition is an English club, they will qualify for the following season's Heineken Cup.
* The Anglo-Welsh Cup is a separate tournament to the British & Irish Cup which involves 12 teams from the English Championship (the level below the Guinness Premiership), six club sides from the Welsh Principality Premiership, Irish provinces Munster, Leinster and Ulster and three Scottish sides.
Billy Burns and Man of the Match Richard Hibbard spoke to GRTV after the 41-0 win in Treviso, on a day when preparations weren't ideal. They felt the team's attitude was key and also praised the travelling support.
Matt Scott was again in impressive try scoring form for the Cherry and Whites on Saturday, and the Scotsman laid praise on his fellow backs for their attacking skills in a big 55-19 win over Worcester Warriors
Jonny May spilled blood for the cause on Saturday, before going on to score the first try in the 55-19 Aviva Premiership victory over Worcester. He told GRTV that the win was a reward for the hard work the squad have been putting in.
Gloucester Rugby face their second tough Aviva Premiership fixture in six days when they take on Worcester Warriors at Kingsholm on Saturday (kick off 3pm). Head Coach Laurie Fisher is expecting a massive challenge from one of Gloucester's local rivals.
It was a frustrated and bitterly disappointed Willi Heinz who spoke to GRTV following the 12-13 reverse to Northampton Saints on New Year's Day. The skipper thanked the fans for their incredible support on a day when Gloucester couldn't quite get the job done.
A postponement, a rescheduling and a 3-hour wait on the tarmac threatened to disrupt Gloucester's trip to Treviso, but a short relocation and delay did nothing to prevent a six-try thumping over Treviso on Saturday afternoon.
It was a case of triumph over adversity for Gloucester in Italy this weekend, their pre-match preparations ruined by a relocation of the game. However, it was a job well done for the Cherry and Whites who defeated Benetton Treviso 41-0 in freezing conditions in Italy to top pool one.