Dean Mumm will lead his country for the first time, while six others could potentially play their maiden matches at senior level for Australia, after all being named in the Qantas Wallabies side to play Gloucester Rugby tomorrow night.
Mumm, 25, leads a run on combination which will see Brumbies inside centre Tyrone Smith, NSW Waratahs fullback Kurtley Beale, NSW Waratahs lock Dave Dennis and Brumbies tighthead prop Salesi Ma'afu all play their first matches for the Wallabies.
This quartet could be joined in the first timers club by Queensland Reds halfback Richard Kingi and Brumbies flyhalf Matt Toomua, if this year's Australia Under-20 halfback and flyhalf pairing are both called on from the bench during the non-cap match.
Flankers Matt Hodgson, unsighted since being injured in June's season opener against the Barbarians, and Mitchell Chapman, who appeared in then Australian A colours during the 2006 Spring Tour, are also notable inclusions.
The announcement of today's line-up means that all 34 players in the Qantas Wallabies touring party will have received match involvement at the conclusion of the first two matches of the seven-match Spring tour.
And while the Gloucester fixture follows hard on the heels of last weekend's final Bledisloe Cup Test match of the year in Tokyo, the Australian starting XV that will take the field for the midweek tour match still features nine players with Test experience and a combined total of 136 Test caps.
Centre Ryan Cross is the only player required to back up in the run on XV after having started against the All Blacks in Tokyo last weekend.
Qantas Wallabies coach Robbie Deans says this situation was necessitated by the injuries which disrupted the squad in the lead-up to last week's Test match, forcing midfield backs Berrick Barnes and Rob Horne to return home.
"We had intended to avoid backing anyone up in the midweek fixture who had started against the All Blacks, but our injury circumstance in the midfield meant that Ryan was required to start," Deans says.
"We did look at starting Matt Toomua, but as he only joined the squad on Sunday when we arrived in London, and would only have the benefit of one day of preparation with the team, we felt that backing up Ryan was the better option. He got through the game last Saturday well and indicated to me straight after the Test match that he was keen to play."
Five members of the bench from last weekend's Test are included among the run on group, while four of tomorrow night's reserves were also involved in Tokyo.
The Qantas Wallabies team to play Gloucester at the Kingsholm Ground, Gloucester, on 3 November, 7.45pm kick off (6.45am, 4 November, AEST), is:
15. Kurtley Beale (NSW Waratahs)
14. Lachie Turner (NSW Waratahs)
13. Ryan Cross (Western Force)
12. Tyrone Smith (Brumbies)
11. Drew Mitchell (NSW Waratahs)
10. Quade Cooper (Queensland Reds)
9. Luke Burgess (NSW Waratahs)
8. Richard Brown (Western Force)
7. Matt Hodgson (Western Force)
6. Mitchell Chapman (Brumbies)
5. Dean Mumm (NSW Waratahs, captain)
4. Dave Dennis (NSW Waratahs)
3. Salesi Ma'afu (Brumbies)
2. Tatafu Polota Nau (NSW Waratahs)
1. Sekope Kepu (NSW Waratahs)
Run on reserves:
16. Pek Cowan (Western Force)
17. Matt Dunning (Western Force)
18. Mark Chisholm (Brumbies)
19. Wycliff Palu (NSW Waratahs)
20. Richard Kingi (Queensland Reds)
21. Matt Toomua (Brumbies)
22. James O'Connor (Western Force)
Skipper Billy Twelvetrees led Gloucester Rugby to European glory on Friday evening, scoring the crucial try on a memorable night for the Cherry and Whites. After the game, he paid tribute to his team's performance under pressure in the latter stages as they withstood a ferocious late Edinburgh fightback.
Speaking to GRTV, James Hook admitted that, after a superb opening, Gloucester really had to pass a test of character to hold off Edinburgh and lift the European Rugby Challenge Cup. He paid tribute to his team mates for all their hard work on what was a great day for the club.
Speaking to the assembled media this week, Gloucester Rugby club captain Billy Twelvetrees explains that the squad are ready for the final and that it will be an honour to lead the Cherry and Whites out in the Challenge Cup final.
Gloucester Rugby scrum half Greig Laidlaw will line up against former team mates Edinburgh Rugby on Friday evening, but the focus is firmly on his own roles as the Cherry and Whites gear up for the EPCR Challenge Cup final at The Stoop.
Speaking to the media ahead of Friday evening's European Rugby Challenge Cup Final against Edinburgh, Director of Rugby David Humphreys stressed that his improving Gloucester team will need to perform at their best to prevail against a tough Edinburgh side.
Dan Robson, a second half try scorer in the 42-40 win over Newcastle on Saturday, admitted that Gloucester had started slowly but that the second half comeback showed real character and maintains momentum going into Friday's European final.
Gareth Evans was a first half replacement for the Cherry and Whites on Saturday afternoon, but the back rower's impressive carrying kept the hosts on the front foot when they needed it most to snatch a dramatic comeback win.
After his last ditch try clinched the 42-40 Aviva Premiership victory over Newcastle at Kingsholm on Saturday, Billy Burns told GRTV that all the Gloucester team deserve credit for hanging in there against a Falcons team who really made life tough for the Cherry and Whites.
Argentine Mariano Galarza was a key part of the Cherry and White squad that tasted silverware on Friday night as they lifted the EPCR Challenge Cup at The Stoop, a special night that will live long in the memory of the second row.
Figures released by EPCR following Gloucester winning the European Rugby Challenge Cup on Friday evening show that, unsurprisingly, a number of those wearing Cherry and White showed up well in the various statistical categories.