Dean Mumm will lead his country for the first time, while six others could potentially play their maiden matches at senior level for Australia, after all being named in the Qantas Wallabies side to play Gloucester Rugby tomorrow night.
Mumm, 25, leads a run on combination which will see Brumbies inside centre Tyrone Smith, NSW Waratahs fullback Kurtley Beale, NSW Waratahs lock Dave Dennis and Brumbies tighthead prop Salesi Ma'afu all play their first matches for the Wallabies.
This quartet could be joined in the first timers club by Queensland Reds halfback Richard Kingi and Brumbies flyhalf Matt Toomua, if this year's Australia Under-20 halfback and flyhalf pairing are both called on from the bench during the non-cap match.
Flankers Matt Hodgson, unsighted since being injured in June's season opener against the Barbarians, and Mitchell Chapman, who appeared in then Australian A colours during the 2006 Spring Tour, are also notable inclusions.
The announcement of today's line-up means that all 34 players in the Qantas Wallabies touring party will have received match involvement at the conclusion of the first two matches of the seven-match Spring tour.
And while the Gloucester fixture follows hard on the heels of last weekend's final Bledisloe Cup Test match of the year in Tokyo, the Australian starting XV that will take the field for the midweek tour match still features nine players with Test experience and a combined total of 136 Test caps.
Centre Ryan Cross is the only player required to back up in the run on XV after having started against the All Blacks in Tokyo last weekend.
Qantas Wallabies coach Robbie Deans says this situation was necessitated by the injuries which disrupted the squad in the lead-up to last week's Test match, forcing midfield backs Berrick Barnes and Rob Horne to return home.
"We had intended to avoid backing anyone up in the midweek fixture who had started against the All Blacks, but our injury circumstance in the midfield meant that Ryan was required to start," Deans says.
"We did look at starting Matt Toomua, but as he only joined the squad on Sunday when we arrived in London, and would only have the benefit of one day of preparation with the team, we felt that backing up Ryan was the better option. He got through the game last Saturday well and indicated to me straight after the Test match that he was keen to play."
Five members of the bench from last weekend's Test are included among the run on group, while four of tomorrow night's reserves were also involved in Tokyo.
The Qantas Wallabies team to play Gloucester at the Kingsholm Ground, Gloucester, on 3 November, 7.45pm kick off (6.45am, 4 November, AEST), is:
15. Kurtley Beale (NSW Waratahs)
14. Lachie Turner (NSW Waratahs)
13. Ryan Cross (Western Force)
12. Tyrone Smith (Brumbies)
11. Drew Mitchell (NSW Waratahs)
10. Quade Cooper (Queensland Reds)
9. Luke Burgess (NSW Waratahs)
8. Richard Brown (Western Force)
7. Matt Hodgson (Western Force)
6. Mitchell Chapman (Brumbies)
5. Dean Mumm (NSW Waratahs, captain)
4. Dave Dennis (NSW Waratahs)
3. Salesi Ma'afu (Brumbies)
2. Tatafu Polota Nau (NSW Waratahs)
1. Sekope Kepu (NSW Waratahs)
Run on reserves:
16. Pek Cowan (Western Force)
17. Matt Dunning (Western Force)
18. Mark Chisholm (Brumbies)
19. Wycliff Palu (NSW Waratahs)
20. Richard Kingi (Queensland Reds)
21. Matt Toomua (Brumbies)
22. James O'Connor (Western Force)
In the second episode in the series of videos where Gloucester Rugby TV chat to box holders at Kingsholm, after they note twenty years as owners of a box. Nick Broady of Avenue Cars and Paul Baldwin of Brunsdons chat to the camera about their memories and about rugby in general.
Gary Jones of Glevum Windows and Conservatories recently visited Kingsholm Stadium and the Cherry and White supporter spoke exclusively to Gloucester Rugby TV after marking two decades as a box holder in the Stowford Press Stand at Kingsholm. A great feat!
Speaking to the local media this week during preseason, new hooker Richard Hibbard said he was really enjoying putting in the hard work and being back to full fitness ahead of the 2014-15 season with the Cherry and Whites.
Nick Walshe was unveiled as Gloucester's new Backs and Attack Coach this week and spoke to Simon Ward for the RFU.com podcast about his excitement at joining Gloucester and working with a new group of players.
Speaking after the Third Test defeat to New Zealand, Ben Morgan told RFU.com that the loss was a disappointing way to sign off the summer tour but that the England team know what they have to do to be successful.
At the end of a tour when there have been so many positives for England, fly half Freddie Burns, who will start the third Test against New Zealand, was clear that the whole squad are focussed on signing off with a memorable win when he spoke to RFU.com
Speaking to Steve Jones of RFU.com straight after the 27-28 defeat to New Zealand on Saturday, Billy Twelvetrees spoke of the frustration of a missed opportunity, but stressed that England would lick their wounds and bounce back.
Freddie Burns contributed 12 points in England's 20-15 defeat against New Zealand on Saturday but, speaking after the game to RFU.com, preferred to talk about the many positives in the performance from Stuart Lancaster's side.
Gloucester number eight Ben Morgan put in a great individual performance as England were just pipped by New Zealand by 20 points to 15 in Auckland on Saturday. He told Steve Jones of RFU.com that the team will be in good spirits going into the 2nd Test in Dunedin.
Thursday evening sees the return of live action to Kingsholm in the form of the Premiership Rugby 7s and the Cherry and Whites have named an exciting squad ready to take on Bath Rugby, Exeter Chiefs and London Irish in Pool B.