All the children taking part were sponsored and the players, as well as getting involved themselves, helped to collect the monies raised.
Gloucester Rugby's Community Manager Gary Little was delighted with the outcome:
"It's been a great effort by everyone. So far, we've raised just over £2,500 and there's still money coming in."
"We'll be doing a collection at the Leicester game on Friday and then we'll be making a presentation, alongside representatives from the schools, to Pudsey himself at half fime."
Pictured are Adam Eustace, Nicky Robinson and Mike Tindall who were doing the honours at Abbeymead Primary.
Also pictured is Pudsey Bear who, contrary to rumour, is not a new signing for Gloucester Rugby despite the recent injury list!
Donations to Children in Need can be made by calling 0345 7 33 22 33