Youngsters Freddie Burns and Jonny May are given a chance to shine as they are named to start against Bath on Saturday afternoon.
The match, a joint Testimonial game for Gloucester's Andy Hazell and Bath's Lee Mears, is Gloucester's first preseason friendly of the summer.
As well as Burns and May, there is a first Gloucester outing for summer signings Tom Voyce, Paul Doran-Jones and Dave Attwood with fellow newcomers Darren Dawidiuk and Pierre Capdevielle on the bench.
Tom Voyce; Charlie Sharples, Jonny May, Carlos Spencer, Lesley Vainikolo; Freddie Burns, Dave Lewis; Alasdair Dickinson, Scott Lawson, Paul Doran-Jones, Dave Attwood, Alex Brown, Peter Buxton, Andrew Hazell, Gareth Delve (capt).
Darren Dawidiuk, Pierre Capdevielle, Adam Eustace, Dan Williams, Rory Lawson, Mike Tindall, James Simpson-Daniel, Nick Wood, Marco Bortolami.
Brighton is the venue of choice for England this week as the squad have reported for a training camp to prepare for the upcoming international against Wales. GRTV caught up with Gloucester's Matt Kvesic after the first day's training.
Speaking to GRTV after the game, Gloucester Rugby number eight Ben Morgan reflected on the loss to Northampton Saints, and spoke about what he and the team need to do for the new season when the team return after the summer break.
In only his second Aviva Premiership outing, Lloyd Evans scored his first senior try for Gloucester against Northampton Saints on Saturday and told GRTV afterwards that he enjoyed the experience despite finishing on the losing side.
Gloucester will round off their Aviva Premiership campaign on Saturday with a tough finale against Northampton Saints. Director of Rugby David Humphreys told the local media this week that he's pleased with the team's attitude over the past two games and is looking for more of the same.
Speaking to GRTV after the 12-11 Aviva Premiership victory at Sale Sharks on Friday evening, Matt Kvesic described it as a real team effort with several players having to play out of position due to illness and injury in the ranks.
Despite he and a number of his team mates playing out of position, Greig Laidlaw's four penalties were enough to help Gloucester to a 12-11 Aviva Premiership win at Sale on Friday evening. The skipper told GRTV after the game that everyone had played their part, especially the younger players.
Ross Moriarty played the full 80 minutes for Wales, while Matt Kvesic was a second half replacement for England as Eddie Jones' side defeated Warren Gatland's men by 27 points to 13 at Twickenham on Sunday.