Youngsters Freddie Burns and Jonny May are given a chance to shine as they are named to start against Bath on Saturday afternoon.
The match, a joint Testimonial game for Gloucester's Andy Hazell and Bath's Lee Mears, is Gloucester's first preseason friendly of the summer.
As well as Burns and May, there is a first Gloucester outing for summer signings Tom Voyce, Paul Doran-Jones and Dave Attwood with fellow newcomers Darren Dawidiuk and Pierre Capdevielle on the bench.
Tom Voyce; Charlie Sharples, Jonny May, Carlos Spencer, Lesley Vainikolo; Freddie Burns, Dave Lewis; Alasdair Dickinson, Scott Lawson, Paul Doran-Jones, Dave Attwood, Alex Brown, Peter Buxton, Andrew Hazell, Gareth Delve (capt).
Darren Dawidiuk, Pierre Capdevielle, Adam Eustace, Dan Williams, Rory Lawson, Mike Tindall, James Simpson-Daniel, Nick Wood, Marco Bortolami.
With such a short turnaround between the two games, and Gloucester looking to reintegrate several players into the line-up after the Autumn international series, Tom Savage told GRTV that the Cherry and Whites will keep changes to a minimum for the European Rugby Challenge Cup game in Zebre on Saturday.
Gloucester travel to Italy this weekend to take on Zebre in Round Four of the European Rugby Challenge Cup. The Cherry and Whites defeated the Italian side 35-10 last Sunday, but Director of Rugby David Humphreys is looking for an improved performance in the return fixture.
Skipper Billy Twelvetrees, speaking to GRTV after the 35-10 over Zebre, was happy with the bonus point win over the Italian side but admitted Gloucester will need to tighten up for the return fixture in Parma.
Gloucester Rugby welcome Sione Kalamafoni, Greig Laidlaw and Tom Palmer back to the starting line up and feature David Halaifonua in a matchday squad for the first time, as they resume Aviva Premiership action against Bath Rugby at Kingsholm on Saturday (kick off 5:30pm).