Youngsters Freddie Burns and Jonny May are given a chance to shine as they are named to start against Bath on Saturday afternoon.
The match, a joint Testimonial game for Gloucester's Andy Hazell and Bath's Lee Mears, is Gloucester's first preseason friendly of the summer.
As well as Burns and May, there is a first Gloucester outing for summer signings Tom Voyce, Paul Doran-Jones and Dave Attwood with fellow newcomers Darren Dawidiuk and Pierre Capdevielle on the bench.
Tom Voyce; Charlie Sharples, Jonny May, Carlos Spencer, Lesley Vainikolo; Freddie Burns, Dave Lewis; Alasdair Dickinson, Scott Lawson, Paul Doran-Jones, Dave Attwood, Alex Brown, Peter Buxton, Andrew Hazell, Gareth Delve (capt).
Darren Dawidiuk, Pierre Capdevielle, Adam Eustace, Dan Williams, Rory Lawson, Mike Tindall, James Simpson-Daniel, Nick Wood, Marco Bortolami.
Speaking to the media after the 45-8 preseason friendly victory over Munster, Director of Rugby David Humphreys wasn't getting too carried away, admitting that his team are making progress but that there's still a way to go.
Billy Twelvetrees spoke to Gloucester Rugby TV on Friday ahead of the pre season friendly with Munster Rugby. The club captain is excited to be back in the action at Kingsholm and experience the atmosphere again.
Gloucester Rugby Director of Rugby David Humphreys spoke to the local media after the 62-19 victory over Yorkshire Carnegie, and showed his delight at the first run out and stated there was still lots to work on ahead of the new season
Mark Atkinson made his first appearance in Cherry and White in the pre season victory over Yorkshire Carnegie on Saturday. Speaking to the local media, he was impressed with the first experience of Kingsholm.
James Hook picked himself up a pair of tries on his first appearance in Cherry and Whites during the 62-19 win over Yorkshire Carnegie. Speaking to the media after, the Welshman was thrilled with the first run out as well as the result.
Gloucester Rugby travelled to West Wales for their third and final preseason game ahead of the start of the Aviva Premiership next week. The Scarlets were the opposition and Gloucester left Parc y Scarlets on the wrong end of a 29-24 scoreline.