Youngsters Freddie Burns and Jonny May are given a chance to shine as they are named to start against Bath on Saturday afternoon.
The match, a joint Testimonial game for Gloucester's Andy Hazell and Bath's Lee Mears, is Gloucester's first preseason friendly of the summer.
As well as Burns and May, there is a first Gloucester outing for summer signings Tom Voyce, Paul Doran-Jones and Dave Attwood with fellow newcomers Darren Dawidiuk and Pierre Capdevielle on the bench.
Tom Voyce; Charlie Sharples, Jonny May, Carlos Spencer, Lesley Vainikolo; Freddie Burns, Dave Lewis; Alasdair Dickinson, Scott Lawson, Paul Doran-Jones, Dave Attwood, Alex Brown, Peter Buxton, Andrew Hazell, Gareth Delve (capt).
Darren Dawidiuk, Pierre Capdevielle, Adam Eustace, Dan Williams, Rory Lawson, Mike Tindall, James Simpson-Daniel, Nick Wood, Marco Bortolami.
Gloucester will field a reshuffled side against the Ospreys in the LV= Cup this weekend, but Backs & Attack Coach Nick Walshe stressed that it's a great opportunity for everyone on duty on Saturday and that maintaining the recent winning run is very important.
Skipper Billy Twelvetrees told GRTV after the win in Brive that the game could have been a tough one to get up for, but that his Gloucester team mates did themselves proud on the night and got a big result.
Reflecting on the 31-20 victory in Brive on Thursday evening, Director of Rugby David Humphreys said that clinching a home European Rugby Challenge Cup quarter-final was the overall aim and duly achieved.
Gloucester may have already clinched a home European Rugby Challenge Cup quarter-final, but Director of Rugby David Humphreys wants his side to finish the pool stages on a winning note in Brive on Thursday evening.
Speaking after Saturday's win, James Hudson was happy to record a bonus-point win against a hugely physical Oyonnax side as Gloucester secured their passage into the European Rugby Challenge Cup Quarter Finals
Gloucester Rugby have added to their front row strength in depth for next season with the signings of Paul Doran-Jones, Paddy McAllister and Nicky Thomas from Harlequins, Aurillac and the Ospreys respectively.