Gloucester United bowed out of the Guinness A League on Thursday evening when they finished on the wrong end of a 55-27 scoreline against London Wasps in the semi final at Dry Leas.
The game was effectively over by half time, after Wasps ran in five first-half tries, to lead 41-3 at the break.
The second half was a much more evenly contested affair, but, despite a spritied Gloucester comeback, Wasps ended up comfortable winners, and for the second time running will now face Northampton Wanderers in the two-legged final likely to be played on April the 20th and 27th.
Wasps started well and edged into a 6-0 lead through two Jeremy Staunton penalties before scrum half Joe Simpson scored the first try of the game on 14 minutes.
Staunton then added a superb solo effort as he broke clear before gathering his own chip ahead to score and slotted the conversion himself for a 20-0 lead.
Gloucester fly half Freddie Burns got Gloucester onto the scoreboard with a penalty as half time approached but Wasps centre Will East scored Wasps' third try.
Adam Eustace and Damien Varley were then sinbinned after a dust up and the first half ended poorly for Gloucester as Wasps extended their lead with a penalty try and a fifth score from winger Lachlan Mitchell. 41-3 at the interval.
Replacement Jonny May scored Gloucester's first try early in the second period but it was quickly cancelled out by one from Wasps' Joe Ward which was quickly followed by another from Varley.
Gloucester dug deep and finished well, scoring three late tries from Charlie Sharples, a second from May and the final word went to flanker Richard Jenkins who scored his side's fourth.
Charlie Sharples was amongst the Gloucester try scorers
Speaking ahead of Saturday's Aviva Premiership fixture at home to Newcastle Falcons (kick off 3pm), Director of Rugby David Humphreys emphasised the importance this weekend of Gloucester building on last week's impressive home victory against the Exeter Chiefs.
Speaking to the assembled national and local media, try-scorer Jonny May was delighted and relieved to have tasted victory on Saturday evening, and is looking forward to the European Rugby Challenge Cup final.
Tom Savage delivered a Man of the Match performance against Exeter Chiefs on his birthday on Saturday evening. He spoke to GRTV after the game about the importance of the European Rugby Challenge Cup win.
Speaking to the local media this week, John Afoa described the hard work that the Cherry and White have been putting in and that he is fighting fit ready for Saturday's European Rugby Challenge Cup semi final clash with Exeter Chiefs.
Gloucester Rugby face Exeter Chiefs this Saturday in the European Rugby Challenge Cup semi-final, and Charlie Sharples, who starts at full back is relishing the challenge of the big Kingsholm occasion.
Gloucester meet the Exeter Chiefs for the fourth time in competitive action this weekend, this time in the semi-final of the European Rugby Challenge Cup. Director of Rugby David Humphreys told the local media this week that this is the sort of game that Gloucester want to be involved in on a regular basis and that he and his team are looking forward to it.
Following the 29-26 loss to Harlequins, club captain Billy Twelvetrees spoke of his disappointment that the Cherry and Whites were unable to cash in on the home side's indiscipline and lose a lead on Saturday afternoon.
Number eight Gareth Evans admitted to a collective feeling of frustration as Gloucester lost narrowly at Harlequins on Saturday, but insisted that there are plenty of positives to draw upon ahead of next Saturday's European Rugby Challenge Cup semi-final at home to Exeter.
Gloucester Rugby return to Aviva Premiership action against the Newcastle Falcons on Saturday (Kingsholm, kick off 3pm), and make several changes to the side that defeated Exeter Chiefs at home last weekend.
With a fantastic rugby season coming to a thrilling climax and the prospect of the world's best battling it out in our back garden in September and October, there has never been a better time to quiz your Gloucester Rugby and home nation heroes on what makes them tick.