The game is England's first international of the season as they build towards the annual FIRA/AER European Championship in April.
England played Scotland twice last season - winning on both occasions - but Fletcher says that Sunday's game promises to be a tough encounter.
He said: "Playing Scotland in Scotland is always a challenge and they'll certainly be up for it; we have trained well together and head up to Hamilton full of confidence. We are always looking for the players and the squad to perform to their potential and put the things that we have worked on in training into the game"
Updates of the game will be available on @EnglandRugby.
Name (School, Club)
Lewis Boyce (Prince Henry's, Leeds Carnegie)
Owen Hills (QEW, Leicester Tigers)
Ollie Adams (St Pauls Catholic College, London Irish)
Jack Walker (Prince Henry's, Leeds Carnegie)
Jack Stanley (Truro College, Exeter Chiefs)
Jack Spencer (Hartpury, Gloucester Rugby)
George Nott (Kings School Chester, Sale Sharks)
Stanley South (Whitgift School, Harlequins)
Huw Taylor (Old Swinford Hospital, Worcester Warriors)
Sam Smith (Old Swinford Hospital, Worcester Warriors)
Sam Underhill (Sir Thomas Rich's School, Gloucester Rugby)
Will Safe (Hartpury College, Gloucester Rugby)
Callum Chick (Gosforth Academy, Newcastle Falcons)
Alexander Mitchell (Lymm HS, Sale Sharks)
Jack Maunder (Blundells School & Devon, Exeter Chiefs)
Theo Brophy-Clews (Abingdon School, London Irish)
Kai Little (Hartpury College, Exeter Chiefs)
Harry Mallinder (Rugby School, Northampton Saints)
Rotimi Segun (Stowe School, Northampton Saints)
Lucas Slowik (Filton College, Bristol)
Ollie Thorley (Cheltenham College, Gloucester)
George Perkins (Oaklands College, Saracens)
Taylor Prell (Giggleswick School, Leeds)
Joe Marchant (Peter Symonds, Harlequins)