Gloucester Rugby’s Lewis Ludlow will captain England at Twickenham when Eddie Jones’ side take on Canada on Saturday.
After impressing on his debut last weekend against the USA, Ludlow has kept the captain armband and will earn his second cap at blindside flanker.
Eddie Jones said: “Our focus this camp is being as strong a 36-player squad as we can be and there has been a lot of hard work and intense competition.
“There is a responsibility on the 23 players selected to perform to their personal best.
“We want to go out there and put smiles on the faces on all the supporters watching at Twickenham and at home on Channel 4.”
England v Canada is live on Channel 4, with coverage starting from 2.30pm and kick-off at 3pm on Saturday.
England XV Starters
15. Freddie Steward (Leicester Tigers, 1 cap)
14. Joe Cokanasiga (Bath Rugby, 10 caps)
13. Henry Slade (Exeter Chiefs, 39 caps)
12. Dan Kelly (Leicester Tigers, uncapped)
11. Adam Radwan (Newcastle Falcons, uncapped)
10. Marcus Smith (Harlequins, 1 cap)
9. Harry Randall (Bristol Bears, 1 cap)
1. Ellis Genge (Leicester Tigers, 29 caps)
2. Jamie Blamire (Newcastle Falcons, 1 cap)
3. Joe Heyes (Leicester Tigers, 1 cap)
4. Harry Wells (Leicester Tigers, uncapped)
5. Charlie Ewels (Bath Rugby, 22 caps)
6. Lewis Ludlow (C) (Gloucester Rugby, 1 cap)
7. Sam Underhill (Bath Rugby, 23 caps)
8. Alex Dombrandt (Harlequins, uncapped)
16. Curtis Langdon (Sale Sharks, 1 cap)
17. Beno Obano (Bath Rugby, 2 caps)
18. Paul Hill (Northampton Saints, 5 caps)
19. Callum Chick (Newcastle Falcons, 1 cap)
20. Lewis Ludlam (Northampton Saints, 9 caps)
21. Dan Robson (Wasps, 13 caps)
22. George Furbank (Northampton Saints, 3 caps)
23. Joe Marchant (Harlequins, 5 caps)